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Journal of Assisted Reproduction and Genetics

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01.11.2013 | Technological Innovations

In vitro development of human primordial follicles to preantral stage after vitrification

verfasst von: Fariba Khosravi, Robert L. Reid, Ashraf Moini, Farid Abolhassani, Mojtaba R. Valojerdi, Frederick W. K. Kan

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 11/2013

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Abstract

Purpose

The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue.

Methods

Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for 7 days the morphology and developmental stages of follicles enclosed in fresh and vitrified groups were analyzed.

Results

High proportion of viable follicles in vitrified ovarian strips was obtained. After culturing for 7 days the percentage of secondary and preantral follicles increased significantly (P < 0.05) whereas primordial and transitory follicles showed a significant decrease (P < 0.05) compared to their respective counterparts at day 0 of culture.

Conclusions

Vitrification of ovarian strips with an improved carrier device and culturing of follicles in ovarian strips after warming yielded developed follicles with high viability and morphological integrity that may be suitable for use in fertility preservation among cancer patients.

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Titel: In vitro development of human primordial follicles to preantral stage after vitrification
verfasst von: Fariba Khosravi
Robert L. Reid
Ashraf Moini
Farid Abolhassani
Mojtaba R. Valojerdi
Frederick W. K. Kan
Publikationsdatum: 01.11.2013
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 11/2013
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0105-z

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In vitro development of human primordial follicles to preantral stage after vitrification

Abstract

Purpose

Methods

Results

Conclusions

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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