nach oben

Lasers in Medical Science

Erschienen in:

12.06.2020 | Original Article

In vitro effects of photobiomodulation applied to gingival fibroblasts cultured on titanium and zirconia surfaces and exposed to LPS from Escherichia coli

verfasst von: Taisa Nogueira Pansani, Fernanda Gonçalves Basso, Carlos Alberto de Souza Costa

Erschienen in: Lasers in Medical Science | Ausgabe 9/2020

Einloggen, um Zugang zu erhalten

Abstract

Photobiomodulation (PBM) therapy is used to stimulate cell proliferation and metabolism, as well as reduce inflammatory cytokine synthesis, which plays a main role in the long-term stability of implants. This study assessed the response of gingival fibroblasts cultured on titanium (Ti) and zirconia (ZrO₂), submitted to PBM and exposed to lipopolysaccharide (LPS). Cells seeded on Ti and ZrO₂ were irradiated (InGaAsP; 780 nm, 25 mW) 3 times, using 0.5, 1.5, and 3.0 J/cm² doses, and exposed to Escherichia coli LPS (1 μg/mL). After 24 h, cell viability (alamarBlue, n = 8), interleukin 6 (IL-6) and 8 (IL-8) synthesis (ELISA, n = 6), and IL-6 and vascular endothelial growth factor (VEGF) gene expression (qPCR, n = 5) were assessed and statistically analyzed (one-way ANOVA, α = 0.05). Cell morphology was evaluated by fluorescence microscopy. Increased cell viability occurred in all groups cultured on Ti compared with that of the control, except for cells exposed to LPS. Fibroblasts cultured on ZrO₂ and LPS-exposed exhibited reduced viability. PBM at 3.0 J/cm² and 1.5 J/cm² downregulated the IL-6 synthesis by fibroblasts seeded on Ti and ZrO₂, as well as IL-8 synthesis by cells seeded on ZrO₂. Fibroblasts seeded on both surfaces and LPS-exposed showed increased IL-6 gene expression; however, this activity was downregulated when fibroblasts were irradiated at 3.0 J/cm². Enhanced VEGF gene expression by cells seeded on Ti and laser-irradiated (3.0 J/cm²). Distinct patterns of cytoskeleton occurred in laser-irradiated cells exposed to LPS. Specific parameters of PBM can biomodulate the inflammatory response of fibroblasts seeded on Ti or ZrO₂ and exposed to LPS.

Nur mit Berechtigung zugänglich

Welander M, Abrahamsson I, Berglundh T (2008) The mucosal barrier at implant abutments of different materials. Clin Oral Implants Res 19(7):635–641. https://doi.org/10.1111/j.1600-0501.2008.01543.xCrossRef

Park JC, Kim HM, Ko J (1998) Effects of extracellular matrix constituents on the attachment of human oral epithelial cells at the titanium surface. Int J Oral Maxillofac Implants 13(6):826–836

An N, Rausch-fan X, Wieland M, Matejka M, Andrukhov O, Schedle A (2012) Initial attachment, subsequent cell proliferation/viability and gene expression of epithelial cells related to attachment and wound healing in response to different titanium surfaces. Dent Mater 28(12):1207–1214. https://doi.org/10.1016/j.dental.2012.08.007CrossRef

Moon YH, Yoon MK, Moon JS, Kang JH, Kim SH, Yang HS, Kim MS (2013) Focal adhesion linker proteins expression of fibroblast related to adhesion in response to different transmucosal abutment surfaces. J Adv Prosthodont 5(3):341–350. https://doi.org/10.4047/jap.2013.5.3.341CrossRef

Lin GH, Madi IM (2019) Soft-tissue conditions around dental implants: a literature review. Implant Dent 28(2):138–143. https://doi.org/10.1097/ID.0000000000000871CrossRef

Lindhe J, Meyle J (2008) Group D of European workshop on periodontology. Peri-implant diseases: consensus report of the sixth European workshop on periodontology. J Clin Periodontol 35(8):282–285. https://doi.org/10.1111/j.1600-051X.2008.01283.xCrossRef

Lindhe J, Berglundh T (1998) The interface between mucosa and implant. Periodontol 2000 17:47–54. https://doi.org/10.1111/j.1600-0757.1998.tb00122.xCrossRef

Iglhaut G, Schwarz F, Winter RR, Mihatovic I, Stimmelmayr M, Schliephake H (2014) Epithelial attachment and downgrowth on dental implant abutments. J Esthet Restor Dent 26(5):324–331. https://doi.org/10.1111/jerd.12097CrossRef

Hawkins DH, Abrahamse H (2006) The role of lasers fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium-neon lasers irradiation. Lasers Surg Med 38(1):74–83. https://doi.org/10.1002/lsm.20271CrossRef

10.

Basso FG, Pansani TN, Turrioni APS, Bagnato VS, Hebling J, de Souza Costa CA (2012) In vitro wound healing improvement by low-level laser therapy application in cultured gingival fibroblasts. Int J Dent 2012:719452CrossRef

11.

Basso FG, Oliveira CF, Kurachi C, Hebling J, Costa CAS (2013) Biostimulatory effect of low-level laser therapy on keratinocytes in vitro. Lasers Med Sci 28:367–374. https://doi.org/10.1155/2012/719452CrossRef

12.

Basso FG, Soares DG, Pansani TN, Cardoso LM, Scheffel DL, de Souza Costa CA, Hebling J (2016) Proliferation, migration, and expression of oral-mucosal-healing-related genes by oral fibroblasts receiving low-level laser therapy after inflammatory cytokines challenge. Laser Surg Med 48(10):1006–1014. https://doi.org/10.1002/lsm.22553CrossRef

13.

Pansani TN, Basso FG, Turrioni APS, Soares DG, Hebling J, de Souza Costa CA (2017) Effects of low-level laser therapy and epidermal growth factor on the activities of gingival fibroblasts obtained from young or elderly individuals. Lasers Med Sci 32(1):45–52. https://doi.org/10.1007/s10103-016-2081-xCrossRef

14.

Khadra M, Kasem N, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Laser therapy accelerates initial attachment and subsequent behaviour of human oral fibroblasts cultured on titanium implant material: a scanning electron microscope and histomorphometric analysis. Clin Oral Implants Res 16(2):168–175. https://doi.org/10.1111/j.1600-0501.2004.01092.xCrossRef

15.

Lopes CB, Pinheiro AL, Sathaiah S, da Silva NS, Salgado MA (2007) Infrared laser photobiomodulation (lambda 830 nm) on bone tissue around dental implants: a Raman spectroscopy and scanning electronic microscopy study in rabbits. Photomed Laser Surg 25(2):96–101. https://doi.org/10.1089/pho.2006.2030CrossRef

16.

Thevenot P, Hu W, Tang L (2008) Surface chemistry influences implant biocompatibility. Curr Top Med Chem 8(4):270–280. https://doi.org/10.2174/156802608783790901CrossRef

17.

Pansani TN, Basso FG, Souza IDR, Hebling J, de Souza Costa CA (2019) Characterization of titanium surface coated with epidermal growth factor and its effect on human gingival fibroblasts. Arch Oral Biol 102:48–54. https://doi.org/10.1016/j.archoralbio.2019.03.025CrossRef

18.

Park JH, Olivares-Navarrete R, Baier RE, Meyer AE, Tannenbaum R, Boyan BD, Schwartz Z (2012) Effect of cleaning and sterilization on titanium implant surface properties and cellular response. Acta Biomater 8(5):1966–1975. https://doi.org/10.1016/j.actbio.2011.11.026CrossRef

19.

Pansani TN, Basso FG, Soares DG, Hebling J, Costa CA (2016) Functional differences in gingival fibroblasts obtained from young and elderly individuals. Braz Dent J 27(5):485–491. https://doi.org/10.1590/0103-6440201600993CrossRef

20.

Lins EC, Oliveira CF, Guimarães OC, de Souza Costa CA, Kurachi C, Bagnato VSA (2013) A novel 785-nm laser diode-based system for standardization of cell culture irradiation. Photomed Laser Surg 31(10):466–473. https://doi.org/10.1089/pho.2012.3310CrossRef

21.

Pansani TN, Basso FG, Soares DG, Turrioni APDS, Hebling J, de Souza Costa CA (2018) Photobiomodulation in the metabolism of lipopolysaccharides-exposed epithelial cells and gingival fibroblasts. Photochem Photobiol 94(3):598–603. https://doi.org/10.1111/php.12877CrossRef

22.

Schultz GS, Wysocki A (2009) Interactions between extracellular matrix and growth factors in wound healing. Wound Repair Regen 17(2):153–162. https://doi.org/10.1111/j.1524-475X.2009.00466.xCrossRef

23.

Cho JW, Kim SA, Lee KS (2012) Platelet-rich plasma induces increased expression of G1 cell cycle regulators, type I collagen, and matrix metalloproteinase-1 in human skin fibroblasts. Int J Mol Med 29(1):32–36. https://doi.org/10.3892/ijmm.2011.803CrossRef

24.

Pae A, Lee H, Kim HS, Kwon YD, Woo YH (2009) Attachment and growth behaviour of human gingival fibroblasts on titanium and zirconia ceramic surfaces. Biomed Mater 4(2):025005. https://doi.org/10.1088/1748-6041/4/2/025005CrossRef

25.

Benz K, Schöbel A, Dietz M, Maurer P, Jackowski J (2019) Adhesion behaviour of primary human osteoblasts and fibroblasts on polyether ether ketone compared with titanium under in vitro lipopolysaccharide incubation. Materials (Basel) 27: 12(17). https://doi.org/10.3390/ma12172739

26.

Navolanic PM, Steelman LS, McCubrey JA (2003) EGFR family signaling and its association with breast cancer development and resistance to chemotherapy. Int J Oncol 22(2):237–252. https://doi.org/10.3892/ijo.22.2.237CrossRef

27.

Yuen CY, Wong SL, Lau CW, Tsang SY, Xu A, Zhu Z, Ng CF, Yao X, Kong SK, Lee HK, Huang Y (2012) From skeleton to cytoskeleton: osteocalcin transforms vascular fibroblasts to myofibroblasts via angiotensin II and Toll-like receptor 4. Circ Res 111(3):e55–e66. https://doi.org/10.1161/CIRCRESAHA.112.271361CrossRef

28.

Dale BA (2002) Periodontal epithelium: a newly recognized role in health and disease. Periodontol 30:70–78. https://doi.org/10.1034/j.1600-0757.2002.03007.xCrossRef

29.

Basso FG, Pansani TN, Soares DG, Scheffel DL, Bagnato VS, Costa CAS, Hebling J (2015) Biomodulation of inflammatory cytokines related to oral mucositis by low-level laser therapy. Photochem Photobiol 91(4):952–956. https://doi.org/10.1111/php.12445CrossRef

30.

Silva TA, Garlet GP, Fukada SY, Silva JS, Cunha FQ (2007) Chemokines in oral inflammatory diseases: apical periodontitis and periodontal disease. J Dent Res 86(4):306–319. https://doi.org/10.1177/154405910708600403CrossRef

31.

Garrido PR, Pedroni ACF, Cury DP, Moreira MS, Rosin F, Sarra G, Marques MM (2019) Effects of photobiomodulation therapy on the extracellular matrix of human dental pulp cell sheets. J Photochem Photobiol B 194:149–157. https://doi.org/10.1016/j.jphotobiol.2019.03.017CrossRef

32.

Tang E, Khan I, Andreana S, Arany PR (2017) Laser-activated transforming growth factor-β1 induces human β-defensin 2: implications for laser therapies for periodontitis and peri-implantitis. Periodontal Res 52(3):360–367. https://doi.org/10.1111/jre.12399CrossRef

33.

Arany PR, Cho A, Hunt TD, Sidhu G, Shin K, Hahm E, Huang GX, Weaver J, Chen AC, Padwa BL, Hamblin MR, Barcellos-Hoff MH, Kulkarni AB, Mooney JD (2014) Photoactivation of endogenous latent transforming growth factor-β1 directs dental stem cell differentiation for regeneration. Sci Transl Med 28; 6(238): 238ra69. https://doi.org/10.1126/scitranslmed.3008234

34.

Hameedaldeen A, Liu J, Batres A, Graves GS, Graves DT (2014) FOXO1, TGF-β regulation and wound healing. Int J Mol Sci 15;15(9): 16257-69. https://doi.org/10.3390/ijms150916257

35.

Capp C, Zennig N, Wajner S, Maia AL (2009) The role of vascular endothelial growth factor in tumor. Revista HCPA 29:51–59

36.

das Neves LM, Leite GP, Marcolino AM, Pinfildi CE, Garcia SB, de Araújo JE, Guirro EC (2017) Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine. Lasers Med Sci 32(2):335–341. https://doi.org/10.1007/s10103-016-2118-1CrossRef

37.

de Souza Costa CA, Hebling J, Scheffel DL, Soares DG, Basso FG, Ribeiro AP (2014) Methods to evaluate and strategies to improve the biocompatibility of dental materials and operative techniques. Dent Mater 30(7):769–784. https://doi.org/10.1016/j.dental.2014.04.010CrossRef

Titel: In vitro effects of photobiomodulation applied to gingival fibroblasts cultured on titanium and zirconia surfaces and exposed to LPS from Escherichia coli
verfasst von: Taisa Nogueira Pansani
Fernanda Gonçalves Basso
Carlos Alberto de Souza Costa
Publikationsdatum: 12.06.2020
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 9/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03062-7

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

In vitro effects of photobiomodulation applied to gingival fibroblasts cultured on titanium and zirconia surfaces and exposed to LPS from Escherichia coli

Abstract

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 9/2020

Irradiation with blue light-emitting diode enhances osteogenic differentiation of stem cells from the apical papilla

Comparison of the balafilcon A and samfilcon A lenses on postoperative pain control and epithelial healing time after photorefractive keratectomy: a contralateral eye study

Near-infrared transillumination with high dynamic range imaging for occlusal caries detection in vitro

Identification of oral cancer in OCT images based on an optical attenuation model

Laser Nd:YAG patterning enhance human osteoblast behavior on zirconia implants

Clinical efficacy comparison of low-temperature plasma radiofrequency ablation and Nd:YAG laser in treating recurrent acquired nasolacrimal duct obstruction