Skip to main content
Hauptrubriken
CME Facharzt-Training Webinare Zeitschriften Bücher e.Medpedia Podcast
Service
Jobbörse Info & Hilfe
Fachgebiete
Anästhesiologie Allgemeinmedizin Arbeitsmedizin Augenheilkunde Chirurgie Dermatologie Gynäkologie und Geburtshilfe HNO Innere Medizin Kardiologie Neurologie Onkologie und Hämatologie Orthopädie und Unfallchirurgie Pädiatrie Pathologie Psychiatrie Radiologie Rechtsmedizin Urologie Zahnmedizin
Themen
Klimawandel und Gesundheit Neues aus dem Markt COVID-19 Praxis und Beruf Seltene Erkrankungen GOÄ & EBM Panorama
Sammlungen
Kasuistiken Algorithmen & Infografiken Blickdiagnosen Arthropedia FlapFinder (für Dermatochirurgen) Videos Kongressberichterstattung Medizin für Apothekerinnen und Apotheker Für Ärztinnen und Ärzte in Weiterbildung Für Medizinstudierende

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Konkret geht es um den Diabetes-Patienten mit kardiovaskulären Erkrankungen oder Risiken, die Herzinsuffizienz sowie die kardiovaskuläre Primär- und Sekundärprävention. Sind Sie hier schon auf dem neuesten Stand? Unsere Experten beleuchten, wo und warum das bisherige Procedere geändert werden soll und was in der Praxis sinnvoll ist. Holen Sie sich Ihr Update und 2 CME-Punkte beim MMW-Webinar am 24. April von 17.30 bis 19 Uhr. 
Erweiterte Suche
Anmelden
nach oben
Endocrine
Erschienen in: Endocrine 2/2017

15.07.2016 | Original Article

In vitro effects of zinc, D-aspartic acid, and coenzyme-Q10 on sperm function

verfasst von: Filippo Giacone, Rosita A. Condorelli, Laura M. Mongioì, Valentina Bullara, Sandro La Vignera, Aldo E. Calogero

Erschienen in: Endocrine | Ausgabe 2/2017

Einloggen, um Zugang zu erhalten

Abstract

Reactive oxygen species favor reproductive processes at low concentrations, but damage spermatozoa and decrease their fertilizing capacity at high concentrations. During infection and/or inflammation of the accessory sex glands reactive oxygen species overproduction may occur which, in turn, may negatively impact on sperm motility, sperm DNA fragmentation, and lipid peroxidation. A number of nutraceutical formulations containing antioxidant molecules have been developed to counteract the deleterious effects of the oxidative stress. A recent formulation containing zinc, D-aspartic acid, and coenzyme-Q10 is present in the pharmaceutical market. Based on these premises, the aim of the present study was to evaluate the effects of this combination on spermatozoa in vitro. The study was conducted on 24 men (32.2 ± 5.5 years): 12 normozoospermic men and 12 asthenozoospermic patients. Spermatozoa from each sample were divided into two control aliquots (aliquot A and B) and an aliquot incubated with zinc, D-aspartic acid, and coenzyme-Q10 (aliquot C). After 3 h of incubation, the following parameters were evaluated: progressive motility, number of spermatozoa with progressive motility recovered after swim-up, lipid peroxidation, and DNA fragmentation. Incubation with zinc, D-aspartic acid, and coenzyme-Q10 maintained sperm motility in normozoospermic men (37.7 ± 1.2 % vs. 35.8 ± 2.3 % at time zero) and improved it significantly in asthenozoospermic patients (26.5 ± 1.9 % vs. 18.8 ± 2.0 % at time zero) (p < 0.01). This resulted in a significantly higher (p < 0.01) number of spermatozoa with progressive motility recovered after swim-up in both normozospermic men (4.1 ± 0.9 vs. 3.3 ± 1.0 millions) and asthenozooseprmic patients (3.2 ± 0.8 vs. 1.6 ± 0.5 millions). Finally, a statistically significant lower sperm lipid peroxidation was found after incubation with zinc, D-aspartic acid, and coenzyme-Q10 (p < 0.05) in both normozospermic men (1.0 ± 0.4 % vs. 2.4 ± 0.9 %) and asthenozooseprmic patients (0.2 ± 0.1 % vs. 0.6 ± 0.2 %). No statistically significant effect was observed on sperm DNA fragmentation. This nutraceutical formulation may be indicated in vitro during the separation of the spermatozoa in the assisted reproduction techniques, during which the spermatozoa undergo an increased oxidative stress.
Literatur
1.
T. Kobayashi, T. Miyazaki, M. Natori, S. Nozawa, Protective role of superoxide dismutase in human sperm motility: superoxide dismutase activity and lipid peroxide in human seminal plasma and spermatozoa. Hum. Reprod. 6, 987–991 (1991)CrossRefPubMed
2.
A. Zini, E. de Lamirande, C. Gagnon, Reactive oxygen species in semen of infertile patients: levels of superoxide dismutase- and catalase-like activities in seminal plasma and spermatozoa. Int. J. Androl. 16, 183–188 (1993)CrossRefPubMed
3.
R.J. Aitken, M. Paterson, H. Fisher, D.W. Buckingham, M. van Duin, Redox regulation of tyrosine phosphorylation in human spermatozoa and its role in the control of human sperm function. J. Cell. Sci. 108(Pt 5), 2017–2025 (1995)PubMed
4.
5.
R. Smith, D. Vantman, J. Ponce, J. Escobar, E. Lissi, Total antioxidant capacity of human seminal plasma. Hum. Reprod. 11, 1655–1660 (1996)CrossRefPubMed
6.
G. Lavranos, M. Balla, A. Tzortzopoulou, V. Syriou, R. Angelopoulou, Investigating ROS sources in male infertility: a common end for numerous pathways. Reprod. Toxicol. 34, 298–307 (2012)CrossRefPubMed
7.
R.J. Aitken, J.S. Clarkson, S. Fishel, Generation of reactive oxygen species, lipid peroxidation, and human sperm function. Biol. Reprod. 41, 183–197 (1989)CrossRefPubMed
8.
L. Björndahl, U. Kvist, A model for the importance of zinc in the dynamics of human sperm chromatin stabilization after ejaculation in relation to sperm DNA vulnerability. Syst. Biol. Reprod. Med. 57, 86–92 (2011)CrossRefPubMed
9.
M. Gavella, V. Lipovac, NADH-dependent oxidoreductase (diaphorase) activity and isozyme pattern of sperm in infertile men. Arch. Androl. 28, 135–141 (1992)CrossRefPubMed
10.
N. Garrido, M. Meseguer, C. Simon, A. Pellicer, J. Remohi, Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility. Asian J. Androl. 6, 59–65 (2004)PubMed
11.
C. Gagnon, A. Iwasaki, E. De Lamirande, N. Kovalski, Reactive oxygen species and human spermatozoa. Ann. N. Y. Acad. Sci. 637, 436–444 (1991)CrossRefPubMed
12.
R.J. Aitken, Molecular mechanisms regulating human sperm function. Mol. Hum. Reprod. 3, 169–173 (1997)CrossRefPubMed
13.
M. Attaran, E. Pasqualotto, T. Falcone, J.M. Goldberg, K.F. Miller, A. Agarwal, R.K. Sharma, The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization. Int. J. Fertil. Womens Med. 45, 314–320 (2000)PubMed
14.
E. De Lamirande, H. Jiang, A. Zini, H. Kodama, C. Gagnon, Reactive oxygen species and sperm physiology. Rev. Reprod. 2, 48–54 (1997)CrossRefPubMed
15.
E. De Lamirande, P. Leclerc, C. Gagnon, Capacitation as a regulatory event that primes spermatozoa for the acrosome reaction and fertilization. Mol. Hum. Reprod. 3, 175–194 (1997)CrossRefPubMed
16.
A. Hamada, S.C. Esteves, M. Nizza, A. Agarwal, Unexplained male infertility: diagnosis and management. Int. Braz. J. Urol. 38, 576–594 (2012)CrossRefPubMed
17.
A. Agarwal, A. Mulgund, S. Alshahrani, M. Assidi, A.M. Abuzenadah, R. Sharma, E. Sabanegh, Reactive oxygen species and sperm DNA damage in infertile men presenting with low level leukocytospermia. Reprod. Biol. Endocrinol. 19, 12–126 (2014)
18.
A. Agarwal, R.K. Sharma, R. Sharma, M. Assidi, A.M. Abuzenadah, S. Alshahrani, D. Durairajanayagam, E. Sabanegh, Characterizing semen parameters and their association with reactive oxygen species in infertile men. Reprod. Biol. Endocrinol. 7, 12–33 (2014)
19.
M.H. Hadwan, L.A. Almashhedy, A.R. Alsalman, Study of the effects of oral zinc supplementation on peroxynitrite levels, arginase activity and NO synthase activity in seminal plasma of Iraqi asthenospermic patients. Reprod. Biol. Endocrinol. 12, 1 (2014)CrossRefPubMedPubMedCentral
20.
M. Trottmann, F.M. Köhn, M. Dickmann, C.G. Stief, A.J. Becker, Drug therapy options for oligoasthenoteratozoospermia syndrome. Urologe. A. 50, 8–16 (2011)CrossRefPubMed
21.
K.J. Buhling, E. Laakmann, The effect of micronutrient supplements on male fertility. Curr. Opin. Obstet. Gynecol. 26, 199–209 (2014)CrossRefPubMed
22.
X.J. Shang, L.L. Wang, D.S. Mo, H.C. Cai, D.D. Zheng, Y.Z. Zhou, Effect and safety of L-carnitine in the treatment of idiopathic oligoasthenozoospermia: a systemic review. Zhonghua Nan Ke Xue 21, 65–73 (2015)PubMed
23.
M.G. Showell, R. Mackenzie-Proctor, J. Brown, A. Yazdani, M.T. Stankiewicz, R.J. Hart, Antioxidants for male subfertility. Cochrane Database Syst. Rev. 12, CD007411 (2014)
24.
M. Muratori, P. Piomboni, E. Baldi, E. Filimberti, P. Pecchioli, E. Moretti, L. Gambera, B. Baccetti, R. Biagiotti, G. Forti, M. Maggi, Functional and ultrastructural features of DNA-fragmented human sperm. J. Androl. 21, 903–912 (2000)PubMed
25.
A. Agarwal, D. Durairajanayagam, S.S. du Plessis, Utility of antioxidants during assisted reproductive techniques: an evidence based review. Reprod. Biol. Endocrinol. 24, 12–112 (2014)
26.
A.H. Colagar, E.T. Marzony, M.J. Chaichi, Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men. Nutr. Res. 29, 82–88 (2009)CrossRefPubMed
27.
M.B. Sørensen, M. Stoltenberg, G. Danscher, E. Ernst, Chelation of intracellular zinc ions affects human sperm cell motility. Mol. Hum. Reprod. 5, 338–341 (1999)CrossRefPubMed
28.
A.A. Caldamone, M.K. Freytag, A.T. Cockett, Seminal zinc and male infertility. Urology 13, 280–281 (1979)CrossRefPubMed
29.
U. Kvist, Importance of spermatozoal zinc as temporary inhibitor of sperm nuclear chromatin decondensation ability in man. Acta Physiol. Scand. 109, 79–84 (1980)CrossRefPubMed
30.
U. Kvist, Sperm nuclear chromatin decondensation ability. An in vitro study on ejaculated human spermatozoa. Acta Physiol. Scand. Suppl. 486, 1–24 (1980)PubMed
31.
G.M. Roomans, E. Lundevall, L. Björndahl, U. Kvist, Removal of zinc from subcellular regions of human spermatozoa by EDTA treatment studied by X-ray microanalysis. Int. J. Androl. 5, 478–486 (1982)CrossRefPubMed
32.
L. Björndahl, U. Kvist, Human sperm chromatin stabilization: a proposed model including zinc bridges. Mol. Hum. Reprod. 16, 23–29 (2010)CrossRefPubMed
33.
P.I. Oteiza, K.L. Olin, C.G. Fraga, C.L. Keen, Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes. J. Nutr. 125, 823–829 (1995)PubMed
34.
M.P. Zago, P.I. Oteiza, The antioxidant properties of zinc: interactions with iron and antioxidants. Free Radic. Biol. Med. 31, 266–274 (2001)CrossRefPubMed
35.
L. Yuyan, W. Junqing, Y. Wei, Z. Weijin, G. Ersheng, Are serum zinc and copper levels related to semen quality? Fertil. Steril. 89, 1008–1011 (2008)CrossRefPubMed
36.
S.E. Chia, C.N. Ong, L.H. Chua, L.M. Ho, S.K. Tay, Comparison of zinc concentrations in blood and seminal plasma and the various sperm parameters between fertile and infertile men. J. Androl. 21, 53–57 (2000)PubMed
37.
J.L. Lafond, B. Sele, A. Favier, Concentration of selected metals in normal and pathological human seminal plasma. J. Trace Elem. Electrolytes Health Dis. 2, 19–21 (1988)PubMed
38.
H. Ohide, Y. Miyoshi, R. Maruyama, K. Hamase, R. Konno, D-Amino acid metabolism in mammals: biosynthesis, degradation and analytical aspects of the metabolic study. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 879, 3162–3168 (2011)CrossRefPubMed
39.
G. D’Aniello, S. Ronsini, F. Guida, P. Spinelli, A. D’Aniello, Occurrence of D-aspartic acid in human seminal plasma and spermatozoa: possible role in reproduction. Fertil. Steril. 84, 1444–1449 (2005)CrossRefPubMed
40.
A. Mancini, G. Balercia, Coenzyme Q(10) in male infertility: physiopathology and therapy. Biofactors 37, 374–380 (2011)CrossRefPubMed
41.
G. Balercia, A. Mancini, F. Paggi, L. Tiano, A. Pontecorvi, M. Boscaro, A. Lenzi, G.P. Littarru, Coenzyme Q10 and male infertility. J. Endocrinol. Invest. 32, 626–632 (2009)CrossRefPubMed
42.
G. Balercia, E. Buldreghini, A. Vignini, L. Tiano, F. Paggi, S. Amoroso, G. Ricciardo-Lamonica, M. Boscaro, A. Lenzi, G. Littarru, Coenzyme Q10 treatment in infertile men with idiopathic asthenozoospermia: a placebo-controlled, double-blind randomized trial. Fertil. Steril. 91, 1785–1792 (2009)CrossRefPubMed
43.
A. Lewin, H. Lavon, The effect of coenzyme Q10 on sperm motility and function. Mol. Aspects Med. 18(Suppl), S213–S219 (1997)CrossRefPubMed
44.
World Health Organization. WHO Laboratory Manual For the Examination and Processing of Human Semen. 5th edn. Cambridge University Press, Cambridge, UK (2010)
45.
S. La Vignera, E. Vicari, R.A. Condorelli, R. D’Agata, A.E. Calogero, Male accessory gland infection and sperm parameters (review). Int. J. Androl. 34(5 Pt 2), e330–e347 (2011)CrossRefPubMed
46.
S. La Vignera, R. Condorelli, E. Vicari, R. D’Agata, A.E. Calogero, Diabetes mellitus and sperm parameters. J. Androl. 33, 145–153 (2012)CrossRefPubMed
47.
S. La Vignera, R.A. Condorelli, E. Vicari, R. D’Agata, A.E. Calogero, Sperm DNA damage in patients with chronic viral C hepatitis. Eur. J. Intern. Med. 23, 19–24 (2012)CrossRef
48.
H. Sakamoto, T. Yajima, M. Nagata, T. Okumura, K. Suzuki, Y. Ogawa, Relationship between testicular size by ultrasonography and testicular function: measurement of testicular length, width, and depth in patients with infertility. Int. J. Urol. 15, 529–533 (2008)CrossRefPubMed
49.
S. La Vignera, A.E. Calogero, R. Condorelli, A. Marziani, M.A. Cannizzaro, F. Lanzafame, E. Vicari, Cryptorchidism and its long-term complications. Eur. Rev. Med. Pharmacol. Sci. 13, 351–356 (2009)PubMed
50.
S. La Vignera, R. Condorelli, E. Vicari, R. D’Agata, A.E. Calogero, Effects of varicocelectomy on sperm DNA fragmentation, mitochondrial function, chromatin condensation, and apoptosis. J. Androl. 33, 389–396 (2012)CrossRefPubMed
51.
S. La Vignera, R.A. Condorelli, G. Balercia, E. Vicari, A.E. Calogero, Does alcohol have any effect on male reproductive function? A review of literature. Asian J. Androl. 15, 221–225 (2013)CrossRefPubMed
52.
R. Talevi, V. Barbato, I. Fiorentino, S. Braun, S. Longobardi, R. Gualtieri, Protective effects of in vitro treatment with zinc, D-aspartate and coenzyme q10 on human sperm motility, lipid peroxidation and DNA fragmentation. Reprod. Biol. Endocrinol. 11, 81 (2013)CrossRefPubMedPubMedCentral
53.
J. Wang, J. Wang, J. Liu, S. Wang, J. Pei, Solubility of D-aspartic acid and I-aspartic acid in aqueous salt solutions from (293 to 343) K. J. Chem. Eng. Data 55, 1735–1738 (2010)CrossRef
54.
Y. Zhao, Y.H. Sun, Z.Y. Li, C. Xie, Y. Bao, Z.J. Chen, J.B. Gong, Q.X. Yin, W. Chen, C. Zhang, Solubility measurements and prediction of coenzyme Q10 solubility in different solvent systems. J. Solution Chem. 42, 764–771 (2013)CrossRef
55.
Á.E. Domínguez-Rebolledo, F. Martínez-Pastor, M.R. Fernández-Santos, E. del Olmo, A. Bisbal, J.L. Ros-Santaella, J.J. Garde, Comparison of the TBARS assay and BODIPY C11 probes for assessing lipid peroxidation in red deer spermatozoa. Reprod. Domest. Anim. 45, e360–e368 (2010)CrossRefPubMed
56.
J.F. Brouwers, B.M. Gadella, In situ detection and localization of lipid peroxidation in individual bovine sperm cells. Free Radic. Biol. Med. 35, 1382–1391 (2003)CrossRefPubMed
57.
R.J. Aitken, J.K. Wingate, G.N. De Iuliis, E.A. McLaughlin, Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. Mol. Hum. Reprod. 13, 203–211 (2007)CrossRefPubMed
58.
E. Moretti, L. Mazzi, G. Terzuoli, C. Bonechi, F. Iacoponi, S. Martini, C. Rossi, G. Collodel, Effect of quercetin, rutin, naringenin and epicatechin on lipid peroxidation induced in human sperm. Reprod. Toxicol. 34, 651–657 (2012)CrossRefPubMed
59.
P. Cohen-Bacrie, S. Belloc, Y.J. Menezo, P. Clement, J. Hamidi, M. Benkhalifa, Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients. Fertil. Steril. 91, 1801–1805 (2009)CrossRefPubMed
60.
T. Mann. The Biochemistry of Semen and of the Male Reproductive Tract. Methuen & Co Ltd, London (1964)
61.
C. Foresta, A. Garolla, I. Cosci, M. Menegazzo, M. Ferigo, V. Gandin, L. De Toni, Role of zinc trafficking in male fertility: from germ to sperm. Hum. Reprod. 29, 1134–1145 (2014)CrossRefPubMed
62.
A. Carpino, L. Siciliano, M.F. Petroni, C. De Stefano, S. Aquila, S. Andó, Low seminal zinc bound to high molecular weight proteins in asthenozoospermic patients: evidence of increased sperm zinc content in oligoasthenozoospermic patients. Hum. Reprod. 13, 111–114 (1998)CrossRefPubMed
63.
R.P. Zhao, C.L. Xiong, Zinc content analysis in serum, seminal plasma and spermatozoa of asthenozoospermic and oligoasthenozoospermic patients. Zhonghua Nan Ke Xue 11, 680–682 (2005)PubMed
64.
T.A. Kumosani, M.F. Elshal, A.A. Al-Jonaid, H.S. Abduljabar, The influence of smoking on semen quality, seminal microelements and Ca2+-ATPase activity among infertile and fertile men. Clin. Biochem. 41, 1199–1203 (2008)CrossRefPubMed
65.
C.H. Deng, B. Zheng, S.F. She, A clinical study of biological zinc for the treatment of male infertility with chronic prostatitis. Zhonghua Nan Ke Xue 11, 127–129 (2005)PubMed
66.
R. Stanwell-Smith, S.G. Thompson, A.P. Haines, R.J. Ward, G. Cashmore, J. Stedronska, W.F. Hendry, A comparative study of zinc, copper, cadmium, and lead levels in fertile and infertile men. Fertil. Steril. 40, 670–677 (1983)CrossRefPubMed
67.
C.I. Madding, M. Jacob, V.P. Ramsay, R.Z. Sokol, Serum and semen zinc levels in normozoospermic and oligozoospermic men. Ann. Nutr. Metab. 30, 213–218 (1986)CrossRefPubMed
68.
F.R. Abou-Shakra, N.I. Ward, D.M. Everard, The role of trace elements in male infertility. Fertil. Steril. 52, 307–310 (1989)CrossRefPubMed
69.
M.H. Hadwan, L.A. Almashhedy, A.R. Alsalman, Oral zinc supplementation restores high molecular weight seminal zinc binding protein to normal value in Iraqi infertile men. BMC Urol. 13, 12–32 (2012)
70.
E. Mocchegiani, L. Costarelli, R. Giacconi, C. Cipriano, E. Muti, M. Malavolta, Zinc-binding proteins (metallothionein and α-2 macroglobulin) and lmmunosenescence. Exp. Gerontol. 41, 1094–1107 (2006)CrossRefPubMed
71.
I.M. Ebisch, C.M. Thomas, W.H. Peters, D.D. Braat, R.P. Steegers-Theunissen, The importance of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility. Hum. Reprod. Update 13, 163–174 (2007)CrossRefPubMed
72.
R. Reyes, V.M. Magdaleno, O. Hernández, A. Rosado, N.M. Delgado, Effect of zinc on decondensation of human spermatozoa nuclei by heparin. Arch. Androl. 10, 155–160 (1983)CrossRefPubMed
73.
D. Bagchi, P.J. Vuchetich, M. Bagchi, M.X. Tran, R.L. Krohn, S.D. Ray, S.J. Stohs, Protective effects of zinc salts on TPA-induced hepatic and brain lipid peroxidation, glutathione depletion, DNA damage and peritoneal macrophage activation in mice. Gen. Pharmacol. 30, 43–50 (1998)CrossRefPubMed
74.
M. Gavella, V. Lipovac, In vitro effect of zinc on oxidative changes in human semen. Andrologia 30, 317–323 (1998)CrossRefPubMed
75.
B. Macanovic, M. Vucetic, A. Jankovic, A. Stancic, B. Buzadzic, E. Garalejic, A. Korac, B. Korac, V. Otasevic, Correlation between sperm parameters and protein expression of antioxidative defense enzymes in seminal plasma: a pilot study. Dis. Markers 2015, 436236 (2015)
76.
A.R. Alsalman, L.A. Almashhedy, M.H. Hadwan, Zinc supplementation attenuates lipid peroxidation and increases antiperoxidant activity in seminal plasma of Iraqi asthenospermic men. Life Sci. J. 10, 989–997 (2013)
77.
M.H. Hadwan, L.A. Almashhedy, A.S. Alsalman, The key role of zinc in enhancement of total antioxidant levels in spermatozoa of patients with asthenozoospermia. Am. J. Mol. Cell Biol. 1, 52–61 (2013)
78.
U. Schlattner, M. Tokarska-Schlattner, T. Wallimann, Mitochondrial creatine kinase in human health and disease. Biochim. Biophys. Acta 1762, 164–180 (2006)CrossRefPubMed
79.
G. Macchia, E. Topo, N. Mangano, E. D’Aniello, R. Boni, DL-Aspartic acid administration improves semen quality in rabbit bucks. Anim. Reprod. Sci. 118, 337–343 (2010)CrossRefPubMed
80.
E. Topo, A. Soricelli, A. D’Aniello, S. Ronsini, G. D’Aniello, The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats. Reprod. Biol. Endocrinol. 7, 120 (2009)CrossRefPubMedPubMedCentral
81.
J. Garrido-Maraver, M.D. Cordero, M. Oropesa-Avila, A.F. Vega, M. de la Mata, A.D. Pavon, E. Alcocer-Gomez, C.P. Calero, M.V. Paz, M. Alanis, I. de Lavera, D. Cotan, J.A. Sanchez-Alcazar, Clinical applications of coenzyme Q10. Front. Biosci. (Landmark Ed.) 1, 619–633 (2014)CrossRef
82.
E. De Lamirande, C. Gagnon, Reactive oxygen species and human spermatozoa. II Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J. Androl. 13, 379–386 (1992)PubMed
83.
E. De Lamirande, C. Tsai, A. Harakat, C. Gagnon, Involvement of reactive oxygen species in human sperm arcosome reaction induced by A23187, lysophosphatidylcholine, and biological fluid ultrafiltrates. J. Androl. 19, 585–594 (1998)PubMed
84.
E.Y. Ko, E.S. Sabanegh Jr, A. Agarwal, Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil. Steril. 102, 1518–1527 (2014)CrossRefPubMed
85.
K. Li, Y. Shi, S. Chen, W. Li, X. Shang, Y. Huang, Determination of coenzyme Q10 in human seminal plasma by high-performance liquid chromatography and its clinical application. Biomed. Chromatogr. 20, 1082–1086 (2006)CrossRefPubMed
86.
A. Nadjarzadeh, F. Shidfar, N. Amirjannati, M.R. Vafa, S.A. Motevalian, M.R. Gohari, S.A. NazeriKakhki, M.M. Akhondi, M.R. Sadeghi, Effect of Coenzyme Q10 supplementation on antioxidant enzymes activity and oxidative stress of seminal plasma: a double-blind randomised clinical trial. Andrologia 46, 177–183 (2014)CrossRefPubMed
87.
R. Festa, E. Giacchi, S. Raimondo, L. Tiano, P. Zuccarelli, A. Silvestrini, E. Meucci, G.P. Littarru, A. Mancini, Coenzyme Q10 supplementation in infertile men with low-grade varicocele: an open, uncontrolled pilot study. Andrologia 46, 805–807 (2014)CrossRefPubMed
88.
M.R. Safarinejad, S. Safarinejad, N. Shafiei, S. Safarinejad, Effects of the reduced form of coenzyme Q10 (ubiquinol) on semen parameters in men with idiopathic infertility: a double-blind, placebo controlled, randomized study. J. Urol. 188, 526–531 (2012)CrossRefPubMed
89.
H.Y. Chang, Y.M. Lin, P.C. Hsu, Y.L. Guo, Reduction of sperm motility in a male laboratory worker exposed to solvents: a case study. Environ. Health Perspect. 109, 753–756 (2001)CrossRefPubMedPubMedCentral
Metadaten
Titel
In vitro effects of zinc, D-aspartic acid, and coenzyme-Q10 on sperm function
verfasst von
Filippo Giacone
Rosita A. Condorelli
Laura M. Mongioì
Valentina Bullara
Sandro La Vignera
Aldo E. Calogero
Publikationsdatum
15.07.2016
Verlag
Springer US
Erschienen in
Endocrine / Ausgabe 2/2017
Print ISSN: 1355-008X
Elektronische ISSN: 1559-0100
DOI
https://doi.org/10.1007/s12020-016-1013-7

Weitere Artikel der Ausgabe 2/2017

Endocrine 2/2017 Zur Ausgabe

Original Article

Effects of chronic administration of the phosphodiesterase inhibitor vardenafil on serum levels of adrenal and testicular steroids in men with type 2 diabetes mellitus

Research Letter

A novel MKRN3 nonsense mutation causing familial central precocious puberty

Original Article

Low-after-high glucose down-regulated Cx43 in H9c2 cells by autophagy activation via cross-regulation by the PI3K/Akt/mTOR and MEK/ERK1/2 signal pathways

Editorial

Is subclinical hyperthyroidism a real syndrome, different from overt hyperthyroidism?

Original Article

The expression of monocarboxylate transporters in thyroid carcinoma can be associated with the morphological features of BRAF V600E mutation

Original Article

Interference in ACTH immunoassay negatively impacts the management of subclinical hypercortisolism

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

19.04.2024 | Vorhofflimmern | Nachrichten

Parodontalbehandlung verbessert Prognose bei Katheterablation

18.04.2024 | Arterielle Hypertonie | Nachrichten

Antihypertensiva schützen auch alte Menschen noch vor Demenz

18.04.2024 | Wirbelsäulenmetastase | Nachrichten

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

17.04.2024 | DGIM 2024 | Nachrichten

Denken Sie bei starker Blutung auch an die Hemmkörper-Hämophilie
weitere anzeigen

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen
Bildnachweise