Introduction
Methods
Collection of Fruit
Preparation of Extract
In Vitro Glycation of Bovine Serum Albumin
Determination of Fructosamine
Determination of Protein Carbonyl Content
Thiol Group Estimation
Determination of CML
Statistical Analysis
Compliance with Ethics Guidelines
Results
In Vitro Glycation of Bovine Serum Albumin
Determination of Fructosamine
Experimental groups | Fructosamine (mM) | |||
---|---|---|---|---|
Week 1 | Week 2 | Week 3 | Week 4 | |
BSA/glucose | 64.30 ± 3.2 | 67.43 ± 4.1a
| 69.80 ± 4.5a
| 72.81 ± 8.4a
|
BSA/glucose/FOH (0.30 mg/ml) | 63.40 ± 6.1 | 65.30 ± 8.2 | 68.50 ± 4.2c
| 71.62 ± 5.9 |
BSA/glucose/FOH (0.60 mg/ml) | 62.78 ± 9.0a
| 66.90 ± 2.5b
| 68.10 ± 6.4c
| 71.30 ± 5.4d
|
BSA/glucose/FOH (1.2 mg/ml) | 62.70 ± 7.1a
| 65.56 ± 2.8b
| 68.60 ± 8.4c
| 70.90 ± 1.5d
|
BSA/glucose/FOH (2.5 mg/ml) | 62.20 ± 4.2a
| 65.40 ± 2.5b
| 68.30 ± 9.1c
| 69.60 ± 9.6d
|
BSA/glucose/FOH (5 mg/ml) | 60.08 ± 6.8a
| 64.50 ± 6.1b
| 66.10 ± 7.2c
| 68.31 ± 1.5d
|
BSA/glucose/AG (5 mg/ml) | 59.07 ± 9.4a
| 62.10 ± 1.5b
| 64.90 ± 1.8c
| 67.10 ± 8.5d
|
Effect of FOH on Protein Oxidation
Experimental groups | Protein carbonyl content (nmol/mg protein) | |||
---|---|---|---|---|
Week 1 | Week 2 | Week 3 | Week 4 | |
BSA/glucose | 60.28 ± 3.2 | 66.73 ± 5.2a
| 78.84 ± 8.4a
| 80.29 ± 6.4a
|
BSA/glucose/FOH (0.30 mg/ml) | 59.52 ± 6.1a
| 65.18 ± 9.5b
| 67.10 ± 1.5c
| 71.59 ± 2.5d
|
BSA/glucose/FOH (0.60 mg/ml) | 57.86 ± 9.0a
| 63.10 ± 8.4b
| 65.25 ± 5.6c
| 70.68 ± 7.4d
|
BSA/glucose/FOH (1.2 mg/ml) | 57.44 ± 7.1a
| 61.41 ± 7.2b
| 64.88 ± 8.1c
| 70.10 ± 4.2d
|
BSA/glucose/FOH (2.5 mg/ml) | 56.81 ± 4.2a
| 59.12 ± 2.8b
| 64.07 ± 5.1c
| 69.78 ± 8.3d
|
BSA/glucose/FOH (5 mg/ml) | 55.87 ± 6.8a
| 55.25 ± 9.4b
| 63.87 ± 6.4c
| 67.14 ± 1.8d
|
BSA/glucose/AG (5 mg/ml) | 47.32 ± 9.4a
| 58.71 ± 7.2b
| 61.32 ± 7.5c
| 64.87 ± 8.4d
|
Effect of FOH on Thiol Groups
Experimental groups | Thiol group (nmol/mg protein) | |||
---|---|---|---|---|
Week 1 | Week 2 | Week 3 | Week 4 | |
BSA/glucose | 68.92 ± 2.6 | 61.87 ± 8.5a
| 56.59 ± 5.4a
| 52.57 ± 8.2a
|
BSA/glucose/FOH (0.30 mg/ml) | 68.44 ± 5.1 | 65.58 ± 9.4b
| 60.58 ± 3.4c
| 54.85 ± 9.1 |
BSA/glucose/FOH (0.60 mg/ml) | 69.52 ± 8.4 | 66.58 ± 3.4b
| 61.85 ± 2.9c
| 54.39 ± 3.5d
|
BSA/glucose/FOH (1.2 mg/ml) | 71.25 ± 7.1a
| 66.12 ± 3.5b
| 60.87 ± 7.6c
| 55.95 ± 5.5d
|
BSA/glucose/FOH (2.5 mg/ml) | 70.25 ± 4.3a
| 67.25 ± 9.4b
| 60.58 ± 4.1c
| 55.74 ± 8.7d
|
BSA/glucose/FOH (5 mg/ml) | 72.26 ± 8.3a
| 68.97 ± 7.5b
| 61.10 ± 8.1c
| 56.54 ± 8.4d
|
BSA/glucose/AG (5 mg/ml) | 71.07 ± 7.5a
| 68.85 ± 4.2b
| 61.87 ± 1.1c
| 56.87 ± 7.4d
|