nach oben

Erschienen in:

01.03.2014 | Original Contribution

In vitro investigation of the bioaccessibility of carotenoids from raw, frozen and boiled red chili peppers (Capsicum annuum)

verfasst von: Alessandro Pugliese, Yvonne O’Callaghan, Rosa Tundis, Karen Galvin, Francesco Menichini, Nora O’Brien, Monica Rosa Loizzo

Erschienen in: European Journal of Nutrition | Ausgabe 2/2014

Einloggen, um Zugang zu erhalten

Abstract

Background

Carotenoid-rich foods are associated with antioxidant activity and the ability to alleviate chronic diseases.

Purpose

The present study investigated the effect of processing on the content and bioaccessibility of carotenoids from 13 cultivars of red chili pepper (Capsicum annuum).

Methods

Carotenoids in chili peppers were analyzed before an in vitro digestion process. The portion of carotenoid transferred to the micelle fraction (bioaccessibility) was also quantified.

Results

β-Carotene, β-cryptoxanthin, capsanthin and antheraxanthin were the most abundant carotenoids. Zeaxanthin, violaxanthin, neoxanthin and lutein were detected at lower concentrations. In general, freezing and boiling reduced carotenoid contents. Capsanthin and zeaxanthin had the highest bioaccessibility at an average value from 36 to 40 %, followed by antheraxanthin (26 %). Bioaccessibility of β-cryptoxanthin, violaxanthin and β-carotene was lower, averaging 6.1, 4.8 and 4.0 %, respectively. Neoxanthin and lutein were not detected in micelles. Freezing increased the bioaccessibility of capsanthin, zeaxanthin, antheraxanthin, β-cryptoxanthin and violaxanthin; β-cryptoxanthin bioaccessibility increased and capsanthin and zeaxanthin bioaccessibility decreased following boiling.

Conclusions

Differences in the contents and bioaccessibility of carotenoids in 13 C. annuum cultivars and between the processed methods were herein evidenced.

Nur mit Berechtigung zugänglich

Markus F, Daood HG, Kapitany J, Biacs PA (1999) Change in the carotenoid and antioxidant content of spice red pepper (paprika) as a function of ripening and some technological factors. J Agric Food Chem 47:100–107CrossRef

Rodríguez-Bernaldo de Quirós A, Costa HS (2006) Analysis of carotenoids in vegetable and plasma samples: A review. J Food Comp Anal 97–111

Tundis R, Loizzo MR, Menichini F, Conforti F, De Luca D (2012) Air-dried Capsicum annuum var. acuminatum medium and big: determination of bioactive constituents, antioxidant activity and carbohydrate-hydrolyzing enzymes inhibition. Food Res Int 45:170–176CrossRef

Hornero-Méndez D, Gómez-Ladrón de Guevara R, Mínguez-Mosquera MI (2000) Carotenoid biosynthesis changes in five red pepper (Capsicum annuum L.) cultivars during ripening. Cultivar selection for breeding. J Agric Food Chem 48:3857–3864CrossRef

Rao AV, Rao LG (2007) Carotenoids and human health. Pharmacol Res 55:207–216CrossRef

Faulks RM, Southon S (2005) Challenges to understanding and measuring carotenoid bioavailability. Biochim Biophys Acta 1740:95–100CrossRef

Micozzi MS, Brown ED, Edwards BK, Bieri JG, Taylor PR, Khachik F, Beecher GR, Smith JC Jr (1992) Plasma carotenoid response to chronic intake of selected foods and beta-carotene supplements in men. Am J Clin Nutr 55:1120–1125

de Pee S, West CE, Muhilal, Karyadi D, Hautvast JGAJ (1995) Lack of improvement in vitamin A status with increased consumption of dark-green leafy vegetables. Lancet 346:75–81

Castenmiller JJM, West CM, Linssen JPH, van het Hof KH, Voragen AGJ (1999) The food matrix of spinach is a limiting factor in determining the bioavailability of β-carotene and to a lesser extent of lutein in humans. J Nutr 129:349–355

10.

Roels OA, Trout M, Dujacquier R (1958) Carotene balances on boys in Ruanda where vitamin A deficiency is prevalent. J Nutr 65:115–127

11.

Shiau A, Mobarhan S, Stacewicz-Sapuntzakis M, Mobarhan S, Stacewicz-Sapuntzakis M, Benya R, Liao Y, Ford C, Bowen P, Friedman H, Frommel TO (1994) Assessment of the intestinal retention of beta-carotene in humans. J Am Coll Nutr 13:369–375CrossRef

12.

White WS, Peck KM, Ulman EA, Erdman JW (1993) The ferret as a model for evaluation of the bioavailabilities of all-trans-β-carotene and its isomers. J Nutr 123:1129–1139

13.

Poor CL, Bierer TL, Merchen NR, Fahey GC, Erdman JW (1993) The accumulation of α-and β-carotene in serum and tissues of pre-ruminant calves fed raw and steamed carrot slurries. J Nutr 123:1296–1304

14.

Hedren E, Diaz V, Svanberg U (2002) Estimation of carotenoid accessibility from carrots determined by an in vitro digestion method. Eur J Clin Nutr 56:425–430CrossRef

15.

Van Buggenhout S, Alminger M, Lemmens L, Colle I, Knockaert G, Moelants K, Van Loey A, Hendrickx M (2010) In vitro approaches to estimate the effect of food processing on carotenoid bioavailability need thorough understanding of process induced microstructural changes. Trends in Food Sci Technol 21:607–618CrossRef

16.

O’Connell O, Ryan L, O’Brien N (2007) Effect of fibre on the bioaccessibility of carotenoids from a combination of vegetables. Proc Nutr Soc 66:113ACrossRef

17.

Thakkar S, Maziya-Dixon B, Dixon A, Failla M (2007) β-Carotene micellarization during in vitro digestion and uptake by Caco-2 cells is directly proportional to β-carotene content in different genotypes of cassava. J Nutr 137:2229–2233

18.

Garrett D, Failla M, Sarama R (2000) Estimation of carotenoid bioavailability from fresh stir-fried vegetables using an in vitro digestion/Caco-2 cell culture model. J Nutr Biochem 11:574–580CrossRef

19.

Rock C, Lovalvo J, Emenhiser C, Ruffin M, Flatt S, Schwartz S (1998) Bioavailability of β-carotene is lower in raw than in processed carrots and spinach in women. J Nutr 128:913–916

20.

Parada J, Aguilera JM (2007) Food microstructure affects the bioavailability of several nutrients. J Food Sci 72:R21–R32CrossRef

21.

O’Sullivan L, Galvin K, Aherne SA, O’Brien NM (2010) Effects of cooking on the profile and micellarization of 9-cis-, 13-cis- and all-trans-β-carotene in green vegetables. Food Res Int 43:1130–1135CrossRef

22.

O’Sullivan L, Jiwan M, Daly T, O’Brien NM (2010) Bioaccessibility, uptake, and transport of carotenoids from peppers (Capsicum spp.) using the coupled in vitro digestion and human intestinal Caco-2 cell model. J Agric Food Chem 58:5374–5379CrossRef

23.

O’Connell O, Ryan L, O’Brien NM (2007) Xanthophyll carotenoids are more bioaccessible from fruits than dark green vegetables. Nutr Res 27:258–264CrossRef

24.

O’Sullivan L, Ryan L, Aherne SA, O’Brien NM (2008) Cellular transport of lutein is greater from uncooked rather than cooked spinach irrespective of whether it is fresh, frozen, or canned. Nutr Res 28:532–538CrossRef

25.

Taylor KL, Brackenbridge AE, Vivier MA, Oberhoster A (2006) High-performance liquid chromatography profiling of the major carotenoids in Arabidopsis thaliana leaf tissue. J Chromatog A 1121:83–91CrossRef

26.

Strobel M, Tinz J, Biesalski HK (2007) The importance of beta-carotene as a source of vitamin A with special regard to pregnant and breastfeeding women. Eur J Nutr 46:1–20CrossRef

27.

Stahl W, Sies H (2005) Bioactivity and protective effects of natural carotenoids. Biochim Biophys Acta 1740:101–107CrossRef

28.

West CE, Castenmiller JJ (1998) Quantification of the ‘‘SLAMENGHI’’ factors for carotenoid bioavailability and bioconversion. Int J Vit Nutr Res 68:371–377

29.

Granado F, Olmedilla B, Herrero C, Pérez-Sacristán B, Blanco I, Blázquez S (2006) Bioavailability of carotenoids and tocopherols from broccoli: in vivo and in vitro assessment. Exp Biol Med 231:1733–1738

30.

Khachik F, Goli M, Beecher GR, Holden JM, Lusby WR, Tenorio MD, Barrera MR (1992) Effect of food preparation on qualitative and quantitative distribution of major carotenoid constituents of tomatoes and several green vegetables. J Agric Food Chem 40:390–398CrossRef

31.

Bieri JG, Brown ED, Smith JC (1985) Determination of individual carotenoids in human plasma by high performance. J Liq Chromatogr 8:473–484CrossRef

32.

Castenmiller JJ, West CE (1998) Bioavailability and bioconversion of carotenoids. Annu Rev Nutr 18:19–38CrossRef

33.

Guil-Guerrero JL, Martinez-Guirado C, del Mar Rebolloso-Fuentes M, Carrique-Perez A (2006) Nutrient composition and anti-oxidant activity of 10 pepper (Capsicum annuum) varieties. Eur Food Res Technol 224:1–9CrossRef

34.

Dutta D, Chaudhuri R, Chakraborty R (2009) Degradation of total carotenoids and texture in frozen pumpkins when kept for storage under varying conditions of time and temperature. Int J Food Sci Nutr 60:17–26CrossRef

35.

Oruña-Concha MJ, González-Castro MJ, López- Hernández J, Simal-Lozano J (1997) Effects of freezing on the pigment content in green beans and padrón peppers. Eur Food Res Technol 205:148–152

36.

Rickman JC, Bruhn CM, Barrett DM (2007) Nutritional comparison of fresh, frozen, and canned fruits and vegetables II. Vitamin A and carotenoids, vitamin E, minerals and fiber. J Sci Food Agric 87:1185–1196CrossRef

37.

Pérez-Gálvez A, Mínguez-Mosquera MI (2005) Esterification of xanthophylls and its effect on chemical behavior and bioavailability of carotenoids in the human. Nutr Res 25:631–640CrossRef

38.

Granado-Lorencio F, Olmedilla-Alonso B, Herrero-Barbudo C, Perez-Sacristan B, Blanco-Navarro I, Blazquez-Garcia S (2007) Comparative in vitro bioaccessibility of carotenoids from relevant contributors to carotenoid intake. J Agric Food Chem 55:6387–6394CrossRef

39.

Ryan L, O’Connell O, O’Sullivan L, Aherne SA, O’Brien NM (2008) Micellarisation of carotenoids from raw and cooked vegetables. Plant Foods Hum Nutr 63:127–133CrossRef

40.

Pérez-Gálvez A, Martin Hans D, Sies H, Stahl W (2003) Incorporation of carotenoids from paprika oleoresin into human chylomicrons. Brit J Nutr 89:787–793CrossRef

41.

Oshima S, Ojima F, Sakamoto H, Ishiguro Y, Terao J (1997) Accumulation and clearance of capsanthin in blood plasma after the ingestion of paprika juice in men. J Nutr 127:1475–1479

42.

Eugster CH (1995) Chemical derivatization: microscale tests for the presence of common functional groups in carotenoids. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids: Vol. 1A, isolation and analysis. Birkhäuser-Verlag, Basel, pp 76–77

43.

Breithaupt DE, Bamedi A, Wirt U (2002) Carotenol fatty acid esters: easy substrates for digestive enzymes? Comparative Biochemistry and Physiology B 132:721–728CrossRef

Titel: In vitro investigation of the bioaccessibility of carotenoids from raw, frozen and boiled red chili peppers (Capsicum annuum)
verfasst von: Alessandro Pugliese
Yvonne O’Callaghan
Rosa Tundis
Karen Galvin
Francesco Menichini
Nora O’Brien
Monica Rosa Loizzo
Publikationsdatum: 01.03.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 2/2014
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-013-0555-1

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

24.04.2024 | DGIM 2024 | Kongressbericht | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

In vitro investigation of the bioaccessibility of carotenoids from raw, frozen and boiled red chili peppers (Capsicum annuum)

Abstract

Background

Purpose

Methods

Results

Conclusions

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Myokarditis nach Infekt – richtig schwierig wird es bei Profisportlern

Thrombophiliescreening – Wenn, dann richtig und nur bei therapeutischer Konsequenz

Bei Senioren mit Prostatakarzinom auf Anämie achten!

Wie erfolgreich ist eine Re-Ablation nach Rezidiv?

Update Innere Medizin

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Background

Purpose

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2014

Influencing factors on iodine content of cow milk

Fructooligosaccharide augments benefits of quercetin-3-O-β-glucoside on insulin sensitivity and plasma total cholesterol with promotion of flavonoid absorption in sucrose-fed rats

Recommended number of participants in iodine nutrition studies is similar before and after an iodine fortification programme

Erratum to: Iodine status in Korean preschool children as determined by urinary iodine excretion

Is heme iron intake associated with risk of coronary heart disease? A meta-analysis of prospective studies

Alterations in the antioxidant defense system in prepubertal children with a history of extrauterine growth restriction

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Myokarditis nach Infekt – richtig schwierig wird es bei Profisportlern

Thrombophiliescreening – Wenn, dann richtig und nur bei therapeutischer Konsequenz

Bei Senioren mit Prostatakarzinom auf Anämie achten!

Wie erfolgreich ist eine Re-Ablation nach Rezidiv?

Update Innere Medizin