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2016 | OriginalPaper | Buchkapitel

51. In-vitro-Serumdiagnostik

verfasst von : M. Ollert, Prof. Dr. med., T. Jakob, Prof. Dr. med., H. Renz, Prof. Dr. med.

Erschienen in: Allergologie

Verlag: Springer Berlin Heidelberg

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Zusammenfassung

Die In-vitro-Allergiediagnostik umfasst Labormessverfahren von Parametern aus Körperflüssigkeiten (Serum, Plasma, Nasalsekret, Bronchialsekret etc.) und von zellulären Parametern (z. B. Bestimmung von CD63 auf basophilen Granulozyten nach Allergeninkubation oder allergenspezifische Stimulation von Lymphozyten bei Kontaktallergie). Im Kapitel In-vitro-Serumdiagnostik werden nur die in den Körperflüssigkeiten, zumeist im Serum, nachweisbaren Parameter behandelt. Zu diesen Parametern zählen die serologischen Messverfahren für allergierelevante Antikörper (Gesamt-IgE-Bestimmung, Messung von allergenspezifischem IgE und IgG oder IgG-Subklassen) und die Nachweisverfahren für zelluläre Entzündungsparameter im Serum (Tryptase, eosinophiles kationisches Protein – ECP). Für die Messung dieser Parameter stehen heute mehrere sensitive, teils vollautomatisierte Labortestverfahren zur Verfügung. Eine wissenschaftliche Bewertung aller verfügbaren Testsysteme für die In-vitro-Allergiediagnostik erfolgt regelmäßig in Leitlinien und Stellungnahmen der maßgeblichen internationalen und nationalen Fachgesellschaften.

Agarwal R, Chakrabarti A, Shah A, Gupta D, Meis JF, Guleria R, Moss R, Denning DW; ABPA complicating asthma ISHAM working group (2013) Allergic bronchopulmonary aspergillosis: review of literature and proposal of new diagnostic and classification criteria. Clin Exp Allergy 43: 850–873

Baker DL, Peng K, Cheu M, Kadkhodayan Fischer S (2014) Evaluation of two commercial omalizumab/free IgE immunoassays: implications of use during therapy. Curr Med Res Opin 30: 913–922

Barrera C, Millon L, Rognon B, Quadroni M, Roussel S, Dalphin JC, Court-Fortune I, Caillaud D, Jouneau S, Fellrath JM, Zaugg C, Reboux G, Monod M (2014) Immunoreactive proteins of Saccharopolyspora rectivirgula for farmer’s lung serodiagnosis. Proteomics Clin Appl 8: 971–981

Beyer K, Grabenhenrich L, Härtl M, Beder A, Kalb B, Ziegert M, Finger A, Harandi N, Schlags R, Gappa M, Puzzo L, Röblitz H, Millner-Uhlemann M, Büsing S, Ott H, Lange L, Niggemann B (2015) Predictive values of component-specific IgE for the outcome of peanut and hazelnut food challenges in children. Allergy 70: 90–98

Blank S, Seismann H, Bockisch B, Braren I, Cifuentes L, McIntyre M, Rühl D, Ring J, Bredehorst R, Ollert MW, Grunwald T, Spillner E (2010) Identification, recombinant expression, and characterization of the 100 kDa high molecular weight Hymenoptera venom allergens Api m 5 and Ves v 3. J Immunol 184: 5403–5413

Blank S, Seismann H, Michel Y, McIntyre M, Cifuentes L, Braren I, Grunwald T, Darsow U, Ring J, Bredehorst R, Ollert M, Spillner E (2011) Api m 10, a genuine A. mellifera venom allergen, is clinically relevant but underrepresented in therapeutic extracts. Allergy 66: 1322–1329

Bonadonna P, Perbellini O, Passalacqua G, Caruso B, Colarossi S, Dal Fior D, Castellani L, Bonetto C, Frattini F, Dama A, Martinelli G, Chilosi M, Senna G, Pizzolo G, Zanotti R (2009) Clonal mast cell disorders in patients with systemic reactions to Hymenoptera stings and increased serum tryptase levels. J Allergy Clin Immunol 123: 680–686

Boos AC, Hagl B, Schlesinger A, Halm BE, Ballenberger N, Pinarci M, Heinz V, Kreilinger D, Spielberger BD, Schimke-Marques LF, Sawalle-Belohradsky J, Belohradsky BH, Przybilla B, Schaub B, Wollenberg A, Renner ED (2014) Atopic dermatitis, STAT3- and DOCK8-hyper-IgE syndromes differ in IgE-based sensitization pattern. Allergy 69: 943–953

Braren I, Blank S, Seismann H, Deckers S, Ollert M, Grunwald T, Spillner E (2007) Generation of human monoclonal allergen-specific IgE and IgG antibodies from synthetic antibody libraries. Clin Chem 53: 837–844

Braren I, Greunke K, Pilette C, Mempel M, Grunwald T, Bredehorst R, Ring J, Spillner E, Ollert M (2011) Quantitation of serum IgE by using chimeras of human IgE receptor and avian immunoglobulin domains. Anal Biochem 412: 134–140

Brockow K, Vieluf D, Püschel K, Grosch J, Ring J (1999) Increased postmortem serum mast cell tryptase in a fatal anaphylactoid reaction to nonionic radiocontrast medium. J Allergy Clin Immunol 104: 237–238

Brockow K, Jofer C, Behrendt H, Ring J (2008) Anaphylaxis in patients with mastocytosis: a study on history, clinical features and risk factors in 120 patients. Allergy 63: 226–232

Brockow K, Kneissl D, Valentini L, Zelger O, Grosber M, Kugler C, Werich M, Darsow U, Matsuo H, Morita E, Ring J (2015) Using a gluten oral food challenge protocol to improve diagnosis of wheat-dependent exercise-induced anaphylaxis. J Allergy Clin Immunol 135: 977–984

Bundesärztekammer (BÄK) (2014) Mitteilungen der Bundesärztekammer (BÄK). Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen. Dtsch Ärztebl 111: A1583–A1618

Canonica GW, Ansotegui IJ, Pawankar R, Schmid-Grendelmeier P, van Hage M, Baena-Cagnani CE, Melioli G, Nunes C, Passalacqua G, Rosenwasser L, Sampson H, Sastre J, Bousquet J, Zuberbier T, WAO-ARIA-GA2LEN Task Force (2013) A WAO-ARIA-GA²LEN consensus document on molecular-based allergy diagnostics. World Allergy Organ J 6: 17

Ceska M, Lundkvist U (1972) A new and simple radioimmunoassay method for the determination of IgE. Immunochemistry 9: 1021–1030

Commins SP, Satinover SM, Hosen J, Mozena J, Borish L, Lewis BD, Woodfolk JA, Platts-Mills TA (2009) Delayed anaphylaxis, angioedema, or urticaria after consumption of red meat in patients with IgE antibodies specific for galactose-alpha-1,3-galactose. J Allergy Clin Immunol 123: 426–433

Corren J, Lemanske RF, Hanania NA, Korenblat PE, Parsey MV, Arron JR, Harris JM, Scheerens H, Wu LC, Su Z, Mosesova S, Eisner MD, Bohen SP, Matthews JG (2011) Lebrikizumab treatment in adults with asthma. N Engl J Med 365: 1088–1098

Earle CD, King EM, Tsay A, Pittman K, Saric B, Vailes L, Godbout R, Oliver KG, Chapman MD (2007) High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol 119: 428–433

Ferreira F, Hawranek T, Gruber P, Wopfner N, Mari A (2004) Allergic cross-reactivity: from gene to the clinic. Allergy 59: 243–267

Fischer J, Hebsaker J, Caponetto P, Platts-Mills TA, Biedermann T (2014) Galactose-alpha-1,3-galactose sensitization is a prerequisite for pork-kidney allergy and cofactor-related mammalian meat anaphylaxis. J Allergy Clin Immunol 134: 755–759

Gauvreau GM, O’Byrne PM, Boulet LP, Wang Y, Cockcroft D, Bigler J, FitzGerald JM, Boedigheimer M, Davis BE, Dias C, Gorski KS, Smith L, Bautista E, Comeau MR, Leigh R, Parnes JR (2014) Effects of an anti-TSLP antibody on allergen-induced asthmatic responses. N Engl J Med 370: 2102–2110

Golden DB, Lawrence ID, Hamilton RH, Kagey-Sobotka A, Valentine MD, Lichtenstein LM (1992) Clinical correlation of the venom-specific IgG antibody level during maintenance venom immunotherapy. J Allergy Clin Immunol 90: 386–393

Hamilton RG, Adkinson NF Jr (2004) In vitro assays for the diagnosis of IgE-mediated disorders. J Allergy Clin Immunol 114: 213–225

Hamilton RG, Williams PB (2010) Specific IgE Testing Task Force of the American Academy of Allergy, Asthma & Immunology; American College of Allergy, Asthma and Immunology; Human IgE antibody serology: a primer for the practicing North American allergist/immunologist. J Allergy Clin Immunol 126: 33–38

Hamilton RG, Matsson PNJ, Chan S, van Cleve M, Hovanec-Burns D, Magnusson C, Kleine-Tebbe J, Renz H, Adkinson NF (2015) Analytical performance characteristics, quality assurance and clinical utility of immunological assays for human immunoglobulin E (IgE) antibodies of defined allergen specificities. Third Edition, I/LA20-A3, International CLSI-Guideline, in press

Heaps A, Carter S, Selwood C, Moody M, Unsworth J, Deacock S, Sumar N, Bansal A, Hayman G, El-Shanawany T, Williams P, Kaminski E, Jolles S (2014) The utility of the ISAC allergen array in the investigation of idiopathic anaphylaxis. Clin Exp Immunol 177: 483–490

Hecker J, Diethers A, Schulz D, Sabri A, Plum M, Michel Y, Mempel M, Ollert M, Jakob T, Blank S, Braren I, Spillner E (2012) An IgE epitope of Bet v 1 and fagales PR10 proteins as defined by a human monoclonal IgE. Allergy 67: 1530–1537

Hiller R, Laffer S, Harwanegg C, Huber M, Schmidt WM, Twardosz A, Barletta B, Becker WM, Blaser K, Breiteneder H, Chapman M, Crameri R, Duchêne M, Ferreira F, Fiebig H, Hoffmann-Sommergruber K, King TP, Kleber-Janke T, Kurup VP, Lehrer SB, Lidholm J, Müller U, Pini C, Reese G, Scheiner O, Scheynius A, Shen HD, Spitzauer S, Suck R, Swoboda I, Thomas W, Tinghino R, Van Hage-Hamsten M, Virtanen T, Kraft D, Müller MW, Valenta R (2002) Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment. FASEB J 16: 414–416

Hofmann SC, Fischer J, Eriksson C, Bengtsson Gref O, Biedermann T, Jakob T (2012) Added value of IgE-detection to α/β/γ-gliadin in wheat-dependent exercise-induced anaphylaxis, Allergy 67: 1457–1460

Ishizaka K, Ishizaka T, Terry WD (1967) Antigenic structure of gamma-E-globulin and reaginic antibody. J Immunol 99: 849–858

Jia G, Erickson RW, Choy DF, Mosesova S, Wu LC, Solberg OD, Shikotra A, Carter R, Audusseau S, Hamid Q, Bradding P, Fahy JV, Woodruff PG, Harris JM, Arron JR; Bronchoscopic Exploratory Research Study of Biomarkers in Corticosteroid-refractory Asthma (BOBCAT) Study Group (2012) Periostin is a systemic biomarker of eosinophilic airway inflammation in asthmatic patients. J Allergy Clin Immunol 130: 647–654

Johansson SG, Bennich H (1967) Immunological studies of an atypical (myeloma) immunoglobulin. Immunology 13: 381–394

Kleine-Tebbe J, Vogel L, Crowell DN, Haustein UF, Vieths S (2002) Severe oral allergy syndrome and anaphylactic reactions caused by a Bet v 1-related PR-10 protein in soybean, SAM22. J Allergy Clin Immunol 110: 797–804

Köhler J, Blank S, Müller S, Bantleon F, Frick M, Huss-Marp J, Lidholm J, Spillner E, Jakob T (2014) Component resolution reveals additional major allergens in patients with honeybee venom allergy. J Allergy Clin Immunol 133: 1383–1389

Leung TF, Wong CK, Chan IH, Ip WK, Lam CW, Wong GW (2002) Plasma concentration of thymus and activation-regulated chemokine is elevated in childhood asthma. J Allergy Clin Immunol 110: 404–409

Löwhagen O, Wever AM, Lusuardi M, Moscato G, De Backer WA, Gandola L, Donner CF, Ahlstedt S, Larsson L, Holgate ST (2002) The inflammatory marker serum eosinophil cationic protein (ECP) compared with PEF as a tool to decide inhaled corticosteroid dose in asthmatic patients. Respir Med 96: 95–101

Ludolph-Hauser D, Ruëff F, Fries C, Schöpf P, Przybilla B (2001) Constitutively raised serum concentrations of mast-cell tryptase and severe anaphylactic reactions to Hymenoptera stings. Lancet 357: 361–362

Luengo O, Cardona V (2014) Component resolved diagnosis: when should it be used? Clin Transl Allergy 4: 28

Matsson PNJ, Hamilton RG, Esch RE, Halsey JF, Homburger HA, Kleine-Tebbe J, Mari A, Ownby DR, Reeves JP, Renz H, Vogt RF Jr, Williams PB (2009) Analytical performance characteristics and clinical utility of immunological assays for human IgE antibodies of defined allergen specificities. Approved Guideline. 2nd ed. Wayne (PA): Clinical Laboratory Standards Institute; CLSI document I/LA20-A2; 29: 1–145

Matsuo H, Kohno K, Niihara H, Morita E (2005) Specific IgE determination to epitope peptides of omega-5 gliadin and high molecular weight glutenin subunit is a useful tool for diagnosis of wheat-dependent exercise-induced anaphylaxis. J Immunol 175: 8116–8122

Müller UR, Johansen N, Petersen AB, Fromberg-Nielsen J, Haeberli G (2009) Hymenoptera venom allergy: analysis of double positivity to honey bee and Vespula venom by estimation of IgE antibodies to species-specific major allergens Api m1 and Ves v5. Allergy 64: 543–548

Müller U, Schmid-Grendelmeier P, Hausmann O, Helbling A (2012) IgE to recombinant allergens Api m 1, Ves v 1, and Ves v 5 distinguish double sensitization from crossreaction in venom allergy. Allergy 67: 1069–1073

Ollert M, Blank S (2015) Anaphylaxis to insect venom allergens: role of molecular diagnostics. Curr Allergy Asthma Rep 15:26. DOI 10.1007/s11882-015-0527-z

Ollert M, Mari A (2015) In vitro allergy diagnosis – molecules and component-resolved diagnosis. In: Akdis CA, Agache I (Hrsg) Global atlas of allergy. European Academy of Allergy and Clinical Immunology, Zürich, pp 168–170

Ollert MW, Ring J (2004) An welche Differenzialdiagnosen muss bei einer IgE-Erhöhung gedacht werden? DMW Praxis Plus/Fortbildung kompakt von der Deutschen Medizinischen Wochenschrift 2/2004: 77

Ollert MW, Weissenbacher S, Rakoski J, Ring J (2005) Allergen-specific IgE measured by a continuous random-access immunoanalyzer: interassay comparison and agreement with skin testing. Clin Chem 51: 1241–1249

Petersen AB, Gudmann P, Milvang-Grønager P, Mørkeberg R, Bøgestrand S, Linneberg A, Johansen N (2004) Performance evaluation of a specific IgE assay developed for the ADVIA centaur immunoassay system. Clin Biochem 37: 882–892

Platts-Mills TA, Woodfolk JA, Erwin EA, Aalberse R (2004) Mechanisms of tolerance to inhalant allergens: the relevance of a modified Th2 response to allergens from domestic animals. Springer Semin Immunopathol 25: 271–279

Pomponi D, Bernardi ML, Liso M, Palazzo P, Tuppo L, Rafaiani C, Santoro M, Labrada A, Ciardiello MA, Mari A, Scala E (2012) Allergen micro-bead array for IgE detection: a feasibility study using allergenic molecules tested on a flexible multiplex flow cytometric immunoassay. PLoS One 7: e35697

Renz H, Biedermann T, Bufe A, Eberlein B, Jappe U, Ollert M, Petersen A, Kleine-Tebbe J, Raulf-Heimsoth M, Saloga J, Werfel T, Worm M (2010) In-vitro allergy diagnostics. S1-guideline update 061/017, German Society of Allergology and Clinical Immunology (DGAKI, together with ÄDA, GPA, and DDG). J Lab Med 34: 177–195

Ring J, Beyer K, Biedermann T, Bircher A, Duda D, Fischer J, Friedrichs F, Fuchs T, Gieler U, Jakob T, Klimek L, Lange L, Merk HF, Niggemann B, Pfaar O, Przybilla B, Ruëff F, Rietschel E, Schnadt S, Seifert R, Sitter H, Varga E-M, Worm M, Brockow K (2014) Guideline for acute therapy und management of anaphylaxis. S2 guideline of DGAKI, AeDA, GPA, DAAU, BVKJ, ÖGAI, SGAI, DGAI, DGP, DGPM, AGATE and DAAB. Allergo J Int 23: 96–112

Sampson HA (2001) Utility of food-specific IgE concentrations in predicting symptomatic food allergy. J Allergy Clin Immunol 107: 891–896

Seismann H, Blank S, Braren I, Greunke K, Cifuentes L, Grunwald T, Bredehorst R, Ollert M, Spillner E (2010) Dissecting cross-reactivity in hymenoptera venom allergy by circumvention of alpha-1,3-core fucosylation. Mol Immunol 47: 799–808

Sennekamp J, Müller-Wening D, Amthor M, Baur X, Bergmann K-CH, Costabel U, Kirsten D, Koschel D, Kroidl R, Liebetrau G, Nowak D, Schreiber J, Vogelmeier C (2007) Guidelines for Diagnosing Extrinsic Allergic Alveolitis (Hypersensitivity Pneumonitis) (German Extrinsic Allergic Alveolitis Study Group) Pneumologie 61: 52–56

Sicherer SH, Dhillon G, Laughery KA, Hamilton RG, Wood RA (2008) Caution: the Phadia hazelnut ImmunoCAP (f17) has been supplemented with recombinant Cor a 1 and now detects Bet v 1-specific IgE, which leads to elevated values for persons with birch pollen allergy. J Allergy Clin Immunol 122: 413–414

Schwartz LB, Yunginger JW, Miller J, Bokhari R, Dull D (1989) Time course of appearance and disappearance of human mast cell tryptase in the circulation after anaphylaxis. J Clin Invest 83: 1551–1555

Stapel SO, Asero R, Ballmer-Weber BK, Knol EF, Strobel S, Vieths S, Kleine-Tebbe J (2008) EAACI Task Force. Testing for IgG4 against foods is not recommended as a diagnostic tool: EAACI Task Force Report. Allergy 63: 793–796

Valent P, Escribano L, Broesby-Olsen S, Hartmann K, Grattan C, Brockow K, Niedoszytko M, Nedoszytko B, Oude Elberink JN, Kristensen T, Butterfield JH, Triggiani M, Alvarez-Twose I, Reiter A, Sperr WR, Sotlar K, Yavuz S, Kluin-Nelemans HC, Hermine O, Radia D, van Doormaal JJ, Gotlib J, Orfao A, Siebenhaar F, Schwartz LB, Castells M, Maurer M, Horny HP, Akin C, Metcalfe DD, Arock M (2014) Proposed diagnostic algorithm for patients with suspected mastocytosis: a proposal of the European Competence Network on Mastocytosis. Allergy 69: 1267–1274

Valenta R, Twaroch T, Swoboda I (2007) Component-resolved diagnosis to optimize allergen-specific immunotherapy in the mediterranean area. J Investig Allergol Clin Immunol 17 (Suppl 1): 88–92

Vickery BP, Lin J, Kulis M, Fu Z, Steele PH, Jones SM, Scurlock AM, Gimenez G, Bardina L, Sampson HA, Burks AW (2013) Peanut oral immunotherapy modifies IgE and IgG4 responses to major peanut allergens. J Allergy Clin Immunol 131: 128–134

Vos B, Köhler J, Müller S, Stretz E, Ruëff F, Jakob T (2013) Spiking venom with rVes v 5 improves sensitivity of IgE detection in patients with allergy to Vespula venom. J Allergy Clin Immunol 131: 1225–1227

Wenzel S, Ford L, Pearlman D, Spector S, Sher L, Skobieranda F, Wang L, Kirkesseli S, Rocklin R, Bock B, Hamilton J, Ming JE, Radin A, Stahl N, Yancopoulos GD, Graham N, Pirozzi G (2013) Dupilumab in persistent asthma with elevated eosinophil levels. N Engl J Med 368: 2455–2466

Wide L, Bennich H, Johansson SG (1967) Diagnosis of allergy by an in-vitro test for allergen antibodies. Lancet 2: 1105–1107

Wölbing F, Biedermann T (2013) Anaphylaxis: opportunities of stratified medicine for diagnosis and risk assessment. Allergy 68: 1499–1508

Zink A, Gensbaur A, Zirbs M, Seifert F, Suarez IL, Mourantchanian V, Weidinger S, Mempel M, Ring J, Ollert M (2015) Targeting IgE in severe atopic dermatitis with a combination of immunoadsorption and omalizumab. Acta Derm Venereol 2015, doi: 10.2340/00015555-2165 (Epub ahead of print])

Titel: In-vitro-Serumdiagnostik
verfasst von: M. Ollert, Prof. Dr. med.
T. Jakob, Prof. Dr. med.
H. Renz, Prof. Dr. med.
Verlag: Springer Berlin Heidelberg
Buch: Allergologie
Print ISBN: 978-3-642-37202-5

Electronic ISBN: 978-3-642-37203-2

Copyright-Jahr: 2016
DOI: https://doi.org/10.1007/978-3-642-37203-2_51

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Springer Medizin

51. In-vitro-Serumdiagnostik

Zusammenfassung

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HNO-Op. auch mit über 90?

Intrakapsuläre Tonsillektomie gewinnt an Boden

Bilateraler Hörsturz hat eine schlechte Prognose

„Nur wer sich gut aufgehoben fühlt, kann auch für Patientensicherheit sorgen“

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Springer Medizin

Zusammenfassung

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Neu im Fachgebiet HNO

HNO-Op. auch mit über 90?

Intrakapsuläre Tonsillektomie gewinnt an Boden

Bilateraler Hörsturz hat eine schlechte Prognose

„Nur wer sich gut aufgehoben fühlt, kann auch für Patientensicherheit sorgen“

Update HNO