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Aesthetic Plastic Surgery

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12.05.2022 | Original Article

In Vitro Study on the Biological Characteristics of Adipose-Derived Stem Cells from Liposuction Area in Patients with Progressive Hemifacial Atrophy

verfasst von: Qiuni Gao, Zuoliang Qi, Xiaolei Jin, Zhenyu Yang, Xihang Yuan, Yuling Hu, Xiaonan Yang

Erschienen in: Aesthetic Plastic Surgery | Ausgabe 5/2022

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Abstract

Objective

In this study, we investigate the biological characteristics of ADSCs from the liposuction area in patients with hemifacial atrophy in vitro.

Methods

ADSCs were respectively extracted from the donor site of patients with hemifacial atrophy and healthy ones. ADSCs of two groups were respectively tested for proliferation ability, phenotype, multipotency, migration ability, self-repair ability, apoptosis, and autophagy. Exosomes extracted from the supernatant of two groups were detected by NTA particle size, electron microscopy (TEM), and WB for CD63 and TSG10, respectively.

Results

CCK-8 showed a statistically less increase in cell proliferation in PHA-ADSCs after the sixth day. ADSCs in both groups had typical phenotypes and multidirectional abilities. PHA-ADSCs exhibited weaker droplet formation. The cell migration ability in PHA-ADSCs was weaker tested by Transwell assay. The live/dead proportion calculated by ImageJ following calcein-AM/PI double staining revealed live cells in PHA-ADSCs was 46.11% compared with 54.21% in NORM-ADSCs after OGD treatment. A significant down-regulation of ATG7 and ATG12 and a higher percentage of apoptosis were found in PHA-ADSCs. A significant up-regulation of BAX occurred in PHA-ADSCs.ARPC5 expression in the PHA group was extremely distinct down-regulated.CDKN1A and CDKN2A expression in the PHA group was significantly up-regulated.WB analyses confirmed that both groups' ADSCs-Exosomes surface markers CD63 and TSG101 were positively expressed but varied significantly.

Conclusions

PHA-ADSCs exhibited a poorer proliferation ability, higher apoptosis percentage, weaker lipid droplets formation, weaker cell migration, poorer intolerance to OGD, aging earlier, and weaker self-renewal and repairability.PHA-ADSCs-Exosomes showed low expressions of CD63 and TSG101.This study provides strong evidence that the addition of exosomes with specific cytokines can improve the fat survival rate after fat filling in patients with hemifacial atrophy.

No Level Assigned

This journal requires that authors 42 assign a level of evidence to each submission to which 43 Evidence-Based Medicine rankings are applicable. This 44 excludes Review Articles, Book Reviews, and manuscripts 45 that concern Basic Science, Animal Studies, Cadaver 46 Studies, and Experimental Studies. For a full description of 47 these Evidence-Based Medicine ratings, please refer to the 48 Table of Contents or the online Instructions to Authors 49 https://www.springer.com/00266.

Schultz KP, Dong E, Truong TA, Maricevich RS (2019) Parry Romberg syndrome. Clin Plast Surg 46(2):231–237. https://doi.org/10.1016/j.cps.2018.11.007CrossRefPubMed

Deshingkar SA, Barpande SR, Bhavthankar JD et al (2012) Progressive hemifacial atrophy (Parry–Romberg syndrome). Contemp Clin Dent. 3(Suppl 1):S78-81. https://doi.org/10.4103/0976-237X.95111.CrossRefPubMedPubMedCentral

Tolkachjov SN, Patel NG, Tollefson MM (2015) Progressive hemifacial atrophy: a review. Orphanet J Rare Dis 1(10):39. https://doi.org/10.1186/s13023-015-0250-9.CrossRef

Rodby KA, Kaptein YE, Roring J et al (2016) Evaluating autologous lipofilling for Parry–Romberg syndrome-associated defects: a systematic literature review and case report. Cleft Palate Craniofac J 53(3):339–350. https://doi.org/10.1597/14-232CrossRefPubMed

Denadai R, Buzzo CL, Raposo-Amaral CA et al (2019) Facial contour symmetry outcomes after site-specific facial fat compartment augmentation with fat grafting in facial deformities. Plast Reconstr Surg 143(2):544–556. https://doi.org/10.1097/PRS.0000000000005220CrossRefPubMed

Zheng H, Yu Z, Deng M et al (2019) Fat extract improves fat graft survival via proangiogenic, anti-apoptotic and pro-proliferative activities. Stem Cell Res Ther 10(1):174. https://doi.org/10.1186/s13287-019-1290-1.CrossRefPubMedPubMedCentral

Chen Y, Xue K, Zhang X et al (2018) Exosomes derived from mature chondrocytes facilitate subcutaneous stable ectopic chondrogenesis of cartilage progenitor cells. Stem Cell Res Ther 9(1):318CrossRef

Liu H, Zhang M, Shi M et al (2021) Adipose-derived mesenchymal stromal cell-derived exosomes promote tendon healing by activating both SMAD1/5/9 and SMAD2/3. Stem Cell Res Ther 12(1):338. https://doi.org/10.1186/s13287-021-02410-w.CrossRefPubMedPubMedCentral

Chieregato K, Castegnaro S, Madeo D et al (2011) Epidermal growth factor, basic fibroblast growth factor and platelet-derived growth factor-bb can substitute for fetal bovine serum and compete with human platelet-rich plasma in the ex vivo expansion of mesenchymal stromal cells derived from adipose tissue. Cytotherapy 13(8):933–943. https://doi.org/10.3109/14653249.2011.583232CrossRefPubMed

10.

Omar Zaki SS, Kanesan L, Leong MYD et al (2019) The influence of serum-supplemented culture media in a transwell migration assay. Cell Biol Int 43(10):1201–1204. https://doi.org/10.1002/cbin.11122CrossRefPubMed

11.

Gorzelniak K, Janke J, Engeli S, Sharma AM (2001) Validation of endogenous controls for gene expression studies in human adipocytes and preadipocytes. Horm Metab Res 33(10):625–627. https://doi.org/10.1055/s-2001-17911CrossRefPubMed

12.

Guerrerosantos J, Guerrerosantos F, Orozco J (2007) Classification and treatment of facial tissue atrophy in Parry–Romberg disease. Aesthet Plast Surg 31:424–434CrossRef

13.

Hu J, Yin L, Tang X et al (2011) Combined skeletal and soft tissue reconstruction for severe Parry–Romberg syndrome. J Craniofac Surg 22:937–941CrossRef

14.

Raposo-Amaral C, Denadai R, Camargo D et al (2013) Parry–Romberg syndrome: severity of the deformity does not correlate with quality of life. Aesthet Plast Surg 37:792–801CrossRef

15.

Palmero ML, Uziel Y, Laxer RM, et al (2010) En coup de sabre scleroderma and Parry–Romberg syndrome in adolescents: surgical options and patient-related outcomes. J Rheumatol 37(10):2174–2179. https://doi.org/10.3899/jrheum.100062. Epub 2010 Sep 15. Erratum in. (2010): J Rheumatol 37(11):2444.

16.

Saccomanno F, Bernardi C, Vittorini P (1997) The expanded polytetrafluoroethylene (ePTFE) in the surgical treatment of Parry–Romberg syndrome: case report. Aesthet Plast Surg 21:342–345CrossRef

17.

Onesti MG, Monarca C, Rizzo MI et al (2009) Minimally invasive combined treatment for Parry–Romberg syndrome. Aesthet Plast Surg 33(3):452–456. https://doi.org/10.1007/s00266-008-9287-7CrossRef

18.

Ortega VG, Sastoque D (2015) New and successful technique for the management of Parry–Romberg syndrome’s soft tissue atrophy. J Craniofac Surg 26(6):e507–e510. https://doi.org/10.1097/SCS.0000000000002023CrossRefPubMed

19.

Avelar RL, Göelzer JG, Azambuja FG et al (2010) Use of autologous fat graft for correction of facial asymmetry stemming from Parry–Romberg syndrome. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109(2):e20–e25CrossRef

20.

Billings E Jr, May JW Jr (1989) Historical review and present status of free fat graft autotransplantation in plastic and reconstructive surgery. Plast Reconstr Surg 83(2):368–381. https://doi.org/10.1097/00006534-198902000-00033CrossRefPubMed

21.

Leaf N, Zarem HA (1972) Correction of contour defects of the face with dermal and dermal-fat grafts. Arch Surg 105:715–719CrossRef

22.

Chajchir A, Benzaquen I (1986) Liposuction fat grafts in face wrinkles and hemifacial atrophy. Aesthet Plast Surg 10(2):115–117. https://doi.org/10.1007/BF01575279CrossRef

23.

Moscona R, Ullman Y, Har-Shai Y et al (1989) Free-fat injections for the correction of hemifacial atrophy. Plast Reconstr Surg 84:501–507CrossRef

24.

Tobin H, Middleton W (1989) Hemifacial atrophy: a case report of fat transplantation. J Otolaryngol 18:125–127PubMed

25.

Liu SW, Cohen GF (2010) Idiopathic hemi-facial lipoatrophy treated with autologous fat transfer. J Cosmet Dermatol 9(3):226–229. https://doi.org/10.1111/j.1473-2165.2010.00507.xCrossRefPubMed

26.

Alencar JC, Andrade SH, Pessoa SG et al (2011) Autologous fat transplantation for the treatment of progressive hemifacial atrophy (Parry–Romberg syndrome: case report and review of medical literature). An Bras Dermatol 86((4 Suppl 1)):S85–S88. https://doi.org/10.1590/s0365-05962011000700022 (English, Portuguese)CrossRefPubMed

27.

Roddi R, Riggio E, Gilbert PM et al (1994) Clinical evaluation of techniques used in the surgical treatment of progressive hemifacial atrophy. J Craniomaxillofac Surg 22(1):23–32. https://doi.org/10.1016/s1010-5182(05)80292-6CrossRefPubMed

28.

Roddi R, Riggio E, Gilbert P et al (1994) The value of microfat injection ‘“lipofilling”’ in the treatment of soft tissue deformities of the face in Parry–Romberg syndrome. Eur J Plast Surg 17:79–83CrossRef

29.

Cortese A, Savastano G, Felicetta L (2000) Free fat transplantation for facial tissue augmentation. J Oral Maxillofac Surg. 58(2):164–9. https://doi.org/10.1016/s0278-2391(00)90331-8 (discussion 169-70)CrossRefPubMed

30.

Rupnick MA, Panigrahy D, Zhang CY et al (2002) Adipose tissue mass can be regulated through the vasculature. Proc Natl Acad Sci USA 99(16):10730–5. https://doi.org/10.1073/pnas.162349799CrossRefPubMedPubMedCentral

31.

Eto H, Kato H, Suga H et al (2012) The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg 129(5):1081–1092. https://doi.org/10.1097/PRS.0b013e31824a2b19CrossRefPubMed

32.

Yoshimura K, Sato K, Aoi N et al (2008) Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg 34(9):1178–1185. https://doi.org/10.1111/j.1524-4725.2008.34256.xCrossRefPubMed

33.

Chang Q, Li J, Dong Z, Liu L, Lu F (2013) Quantitative volumetric analysis of progressive hemifacial atrophy corrected using stromal vascular fraction-supplemented autologous fat grafts. Dermatol Surg 39(10):1465–1473. https://doi.org/10.1111/dsu.12310CrossRefPubMed

34.

Cervelli V, Gentile P (2009) Use of cell fat mixed with platelet gel in progressive hemifacial atrophy. Aesthet Plast Surg 33(1):22–27. https://doi.org/10.1007/s00266-008-9223-xCrossRef

35.

Tiryaki T, Findikli N, Tiryaki D (2011) Staged stem cell-enriched tissue (SET) injections for soft tissue augmentation in hostile recipient areas: a preliminary report. Aesthet Plast Surg 35(6):965–971. https://doi.org/10.1007/s00266-011-9716-xCrossRef

36.

Zhu M, Zhou Z, Chen Y et al (2010) Supplementation of fat grafts with adipose-derived regenerative cells improves long-term graft retention. Ann Plast Surg 64(2):222–228. https://doi.org/10.1097/SAP.0b013e31819ae05cCrossRefPubMed

37.

CastroGovea Y, De La Garza-Pineda O, Lara-Arias J et al (2012) Cell-assisted lipotransfer for the treatment of Parry–Romberg syndrome. Arch Plast Surg 39(6):659–62. https://doi.org/10.5999/aps.2012.39.6.659CrossRef

38.

Doi K, Ogata F, Eto H et al (2015) Differential contributions of graft-derived and host-derived cells in tissue regeneration/remodeling after fat grafting. Plast Reconstr Surg 135(6):1607–1617. https://doi.org/10.1097/PRS.0000000000001292CrossRefPubMed

39.

Shih YC, Lee PY, Cheng H et al (2013) Adipose-derived stem cells exhibit antioxidative and antiapoptotic properties to rescue ischemic acute kidney injury in rats. Plast Reconstr Surg 132(6):940e–951e. https://doi.org/10.1097/PRS.0b013e3182a806ceCrossRefPubMed

40.

Yuan YI, Gao J, Lu F (2015) Effect of exogenous adipose-derived stem cells in the early stages following free fat transplantation. Exp Ther Med 10(3):1052–1058. https://doi.org/10.3892/etm.2015.2605CrossRefPubMedPubMedCentral

41.

Li C, Chen K, Jia M et al (2018) AMPK promotes survival and adipogenesis of ischemia-challenged ADSCs in an autophagy-dependent manner. Biochim Biophys Acta Mol Cell Biol Lipids 1863(12):1498–1510. https://doi.org/10.1016/j.bbalip.2018.10.002CrossRefPubMed

42.

Rosen ED, MacDougald OA (2006) Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 7(12):885–896. https://doi.org/10.1038/nrm2066CrossRefPubMed

43.

Cristancho AG, Lazar MA (2011) Forming functional fat: a growing understanding of adipocyte differentiation. Nat Rev Mol Cell Biol 12(11):722–734. https://doi.org/10.1038/nrm3198CrossRefPubMedPubMedCentral

44.

Isakovic AM, Dulovic M, Markovic I et al (2017) Autophagy suppression sensitizes glioma cells to IMP dehydrogenase inhibition-induced apoptotic death. Exp Cell Res 350(1):32–40. https://doi.org/10.1016/j.yexcr.2016.11.001CrossRefPubMed

45.

Jung CH, Ro SH, Cao J, Otto NM, Kim DH (2010) mTOR regulation of autophagy. FEBS Lett. 584(7):1287–95. https://doi.org/10.1016/j.febslet.2010.01.017CrossRefPubMedPubMedCentral

46.

Ariosa AR, Klionsky DJ (2016) Autophagy core machinery: overcoming spatial barriers in neurons. J Mol Med. 94(11):1217–1227. https://doi.org/10.1007/s00109-016-1461-9CrossRefPubMed

47.

Dejean LM, Martinez-Caballero S, Guo L et al (2005) Oligomeric Bax is a component of the putative cytochrome c release channel MAC, mitochondrial apoptosis-induced channel. Mol Biol Cell. 16(5):2424–32. https://doi.org/10.1091/mbc.e04-12-1111CrossRefPubMedPubMedCentral

48.

Kinoshita T, Nohata N, Watanabe-Takano H et al (2012) Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma. Int J Oncol 40(6):1770–1778. https://doi.org/10.3892/ijo.2012.1390CrossRefPubMed

49.

Hayflick L (1965) The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 37:614–636. https://doi.org/10.1016/0014-4827(65)90211-9CrossRefPubMed

50.

Munk R, Panda AC, Grammatikakis I et al (2017) Senescence-associated MicroRNAs. Int Rev Cell Mol Biol 334:p177-205. https://doi.org/10.1016/bs.ircmb.2017.03.008CrossRef

51.

Ohtani N, Takahashi A, Mann DJ et al (2012) Cellular senescence: a double-edged sword in the fight against cancer. Exp Dermatol 21(Suppl 1):1–4. https://doi.org/10.1111/j.1600-0625.2012.01493.xCrossRefPubMed

52.

He C, Zheng S, Luo Y, Wang B (2018) Exosome theranostics: biology and translational medicine. Theranostics 8(1):237–255. https://doi.org/10.7150/thno.21945CrossRefPubMedPubMedCentral

Titel: In Vitro Study on the Biological Characteristics of Adipose-Derived Stem Cells from Liposuction Area in Patients with Progressive Hemifacial Atrophy
verfasst von: Qiuni Gao
Zuoliang Qi
Xiaolei Jin
Zhenyu Yang
Xihang Yuan
Yuling Hu
Xiaonan Yang
Publikationsdatum: 12.05.2022
Verlag: Springer US
Erschienen in: Aesthetic Plastic Surgery / Ausgabe 5/2022
Print ISSN: 0364-216X
Elektronische ISSN: 1432-5241
DOI: https://doi.org/10.1007/s00266-022-02902-x

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In Vitro Study on the Biological Characteristics of Adipose-Derived Stem Cells from Liposuction Area in Patients with Progressive Hemifacial Atrophy

Abstract

Objective

Methods

Results

Conclusions

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Methods

Results

Conclusions

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