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11.08.2015 | Research Article

In vivo and in vitro effect of hepatocarcinoma lymph node metastasis by upregulation of Annexin A7 and relevant mechanisms

verfasst von: Xian-Yan Wang, Feng Gao, Yu-Rong Sun, Lu-Lu Bai, Mohammed Mohammed Ibrahim, Bo Wang, Jian-Wu Tang

Erschienen in: Tumor Biology | Ausgabe 1/2016

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Abstract

We unveiled the association of Annexin A7 with vascular endothelial growth factor-C (VEGF-C) and the effect of upregulation of Annexin A7 in Hca-F and Hca-P cells on inhibiting hepatocarcinoma (HCC) lymph node metastasis (LNM) in vitro and in vivo. A total of 200 inbred 615 mice were randomly divided into four equal groups inoculated with Hca-F, Hca-P, F_{Anxa7-upregulated}, and P_{Anxa7-upregulated} cells, respectively. The primary tumor, popliteal, inguinal, and iliac lymph nodes were prepared for immunohistochemical (IHC) staining, real-time quantitative polymerase chain reaction (qRT-PCR) analysis, Western blot, and hematoxylin-eosin (H&E) staining. There was over 50 % increase both in the number of F_{Anxa7-upregulated} and P_{Anxa7-upregulated} cells migrated through the filter compared to their controls (F_{Anxa7-control}, Hca-F and P_{Anxa7-control}, Hca-P). However, no significant differences were noted in invasion ability between them (all P > 0.05). Tumor lymph vessels were significantly reduced in F_{Anxa7-upregulated} and P_{Anxa7-upregulated} tumors when compared with Hca-F and Hca-P tumors (all P < 0.05). Blood vessel density did not differ significantly between F_{Anxa7-upregulated} and P_{Anxa7-upregulated} tumors and Hca-F and Hca-P tumors. Enzyme-linked immunosorbent assay (ELISA) for VEGF-C showed that upregulating Annexin A7 decreased VEGF-C secretion in F_{Anxa7-upregulated} and P_{Anxa7-upregulated} cells (P < 0.05). The IHC staining result showed that the level of serum Annexin A7 was found to be statistically higher in all experimental groups than that in the control group (P < 0.05). The present results indicated that alterations in serum Annexin A7 expression may be of prognostic relevance in HCC lymphatic metastasis.

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Titel: In vivo and in vitro effect of hepatocarcinoma lymph node metastasis by upregulation of Annexin A7 and relevant mechanisms
verfasst von: Xian-Yan Wang
Feng Gao
Yu-Rong Sun
Lu-Lu Bai
Mohammed Mohammed Ibrahim
Bo Wang
Jian-Wu Tang
Publikationsdatum: 11.08.2015
Verlag: Springer Netherlands
Erschienen in: Tumor Biology / Ausgabe 1/2016
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-015-3691-9

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In vivo and in vitro effect of hepatocarcinoma lymph node metastasis by upregulation of Annexin A7 and relevant mechanisms

Abstract

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