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01.06.2010 | Original Article | Ausgabe 3/2010

Journal of Nuclear Cardiology 3/2010

In vivo molecular imaging of myocardial angiogenesis using the αvβ3 integrin-targeted tracer 99mTc-RAFT-RGD

Zeitschrift:
Journal of Nuclear Cardiology > Ausgabe 3/2010
Autoren:
MS Julien Dimastromatteo, PhD Laurent M. Riou, PharmD Mitra Ahmadi, MS Guillaume Pons, PhD Eric Pellegrini, PhD Alexis Broisat, PhD Lucie Sancey, MD Tatiana Gavrilina, PhD Didier Boturyn, PhD Pascal Dumy, MD, PhD Daniel Fagret, PhD Catherine Ghezzi
Wichtige Hinweise

Funding

Financial support was provided by the National Institute for Health and Medical Research (INSERM), the National Agency for Research and technology (ANRT), and ERAS Labo. The authors have no conflict of interest to disclose.

Abstract

Background

Myocardial angiogenesis following reperfusion of an infarcted area may impact on patient prognosis and pro-angiogenic treatments are currently evaluated. The non-invasive imaging of angiogenesis would therefore be of potential clinical relevance in these settings. 99mTc-RAFT-RGD is a novel 99mTc-labeled tracer that targets the αvβ3 integrin. Our objective was to determine whether this tracer was suitable for myocardial angiogenesis imaging.

Methods and Results

A rat model of reperfused myocardial infarction was employed. Fourteen days following reperfusion, the animals were injected with 99mTc-RAFT-RGD or with its negative control 99mTc-RAFT-RAD. Fourteen animals were dedicated to autoradiographic imaging, infarct staining, and gamma-well counting of myocardial activity. In vivo dual-isotope pinhole SPECT imaging of 201Tl and 99mTc-RAFT-RGD or 99mTc-RAFT-RAD was also performed in 11 additional animals. Neovessels were observed by immunostaining in the infarcted and peri-infarct areas. 99mTc-RAFT-RGD infarct-to-normal ratios by gamma-well counting and ex vivo imaging (2.5 ± 0.6 and 4.9 ± 0.9, respectively) were significantly higher than those of 99mTc-RAFT-RAD (1.7 ± 0.2 and 2.2 ± 0.4, respectively, P < .05). The infarcted area was readily visible in vivo by SPECT with 99mTc-RAFT-RGD but not with 99mTc-RAFT-RAD (infarct-to-normal zone activity ratio, 2.5 ± 0.6 and 1.7 ± 0.4, respectively, P < .05).

Conclusion

99mTc-RAFT-RGD allowed the experimental in vivo molecular imaging of myocardial angiogenesis.

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