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European Journal of Trauma and Emergency Surgery

Erschienen in:

30.08.2018 | Original Article

Increased extracellular ubiquitin in surgical wound fluid provides a chemotactic signal for myeloid dendritic cells

verfasst von: Maximilian Leiblein, Norbert Ponelies, Theresa Johnson, Julian Marzi, Kerstin Kontradowitz, Emanuel Geiger, Ingo Marzi, Dirk Henrich

Erschienen in: European Journal of Trauma and Emergency Surgery | Ausgabe 1/2020

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Abstract

Purpose

Myeloid dendritic cells (MDC) decline significantly after multiple traumas which might be due to an increased migration into injured regions. Ubiquitin is released from dying cells and is increased in serum after trauma. Ubiquitin can bind to the chemokine receptor CXCR4. Thus, we hypothesized that elevated ubiquitin provides a chemotactic signal for MDC to injured regions.

Methods

Surgical wound fluid (SWF) and serum from patients with mono-trauma (n = 20) were used to simulate the humoral situation in injured tissue. MDC were identified by flow cytometry. Chemotaxis was measured using transwell migration assays. Ubiquitin and CXCL12 (natural CXCR4 ligand) were determined by ELISA.

Results

MDC express CXCR4 and fluorescence-labeled ubiquitin binds to MDC. Ubiquitin exerts a dose-dependent chemotactic effect (fourfold at 100 ng/mL, p < 0.05). Ubiquitin concentration was sixfold higher in SWF (p < 0.05), whereas CXCL12 was increased in serum. MDC migration towards SWF was significantly reduced (− 40%, p < 0.05), if ubiquitin was neutralized by specific antibodies.

Conclusions

Ubiquitin is increased in SWF and exerts a significant chemotactic effect on MDC. This mechanism might play a role in attraction of immune cells to injured regions and might contribute to the decline of circulating MDC in multiple traumas.

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Titel: Increased extracellular ubiquitin in surgical wound fluid provides a chemotactic signal for myeloid dendritic cells
verfasst von: Maximilian Leiblein
Norbert Ponelies
Theresa Johnson
Julian Marzi
Kerstin Kontradowitz
Emanuel Geiger
Ingo Marzi
Dirk Henrich
Publikationsdatum: 30.08.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Trauma and Emergency Surgery / Ausgabe 1/2020
Print ISSN: 1863-9933
Elektronische ISSN: 1863-9941
DOI: https://doi.org/10.1007/s00068-018-1001-0

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Increased extracellular ubiquitin in surgical wound fluid provides a chemotactic signal for myeloid dendritic cells

Abstract

Purpose

Methods

Results

Conclusions

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