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02.11.2015 | Original Article

Increased storage and secretion of phosphatidylcholines by senescent human peritoneal mesothelial cells

verfasst von: Maria Bartosova, Andras Rudolf, Sebastian Pichl, Kathrin Schmidt, Jürgen G. Okun, Beate K. Straub, Rafael Rutkowski, Janusz Witowski, Claus P. Schmitt

Erschienen in: Clinical and Experimental Nephrology | Ausgabe 4/2016

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Abstract

Background/aims

Human peritoneal mesothelial cells (HPMC) secrete phosphatidylcholines (PC) which form a lipid bilayer lining the peritoneum. They prevent frictions and adhesions and act as a barrier to the transport of water-soluble solutes while permitting water flux. PC may play an essential role in peritoneal integrity and function, the role of PD induced HPMC senescence on PC homeostasis, however, is unknown.

Methods

HPMC cell lines were isolated from four non-uremic patients. Expression of the three PC synthesis genes (rt-PCR), and cellular storage and secretion of PC (ESI-mass-spectrometry) were analyzed in young and senescent HPMC (>Hayflick-limit).

Results

Senescent cells displayed significantly altered morphology; flow cytometry demonstrated extensive staining for senescence-associated beta galactosidase. Nine different PC were detected in HPMC with palmitoyl-myristoyl phosphatidylcholine (PMPC) being most abundant. In senescent HPMC mRNA expression of the three key PC synthesis genes was 1.5-, 2.4- and 6-fold increased as compared to young HPMC, with the latter, phosphatidylcholine cytidylyltransferase, being rate limiting. Intracellular storage of the nine PC was 75–450 % higher in senescent vs. young HPMC, PC secretion rates were 100–300 % higher. Intracellular PC concentrations were not correlated with the PC secretion rates. Electron microscopy demonstrated lamellar bodies, the primary storage site of PC, in senescent but not in young cells.

Conclusion

Senescent HPMC store and secrete substantially more PC than young cells. Our findings indicate a novel protective mechanism, which should counteract peritoneal damage induced by chronic exposure to PD fluids.

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Titel: Increased storage and secretion of phosphatidylcholines by senescent human peritoneal mesothelial cells
verfasst von: Maria Bartosova
Andras Rudolf
Sebastian Pichl
Kathrin Schmidt
Jürgen G. Okun
Beate K. Straub
Rafael Rutkowski
Janusz Witowski
Claus P. Schmitt
Publikationsdatum: 02.11.2015
Verlag: Springer Japan
Erschienen in: Clinical and Experimental Nephrology / Ausgabe 4/2016
Print ISSN: 1342-1751
Elektronische ISSN: 1437-7799
DOI: https://doi.org/10.1007/s10157-015-1192-1

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Increased storage and secretion of phosphatidylcholines by senescent human peritoneal mesothelial cells

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Conclusion

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