Hypertension is a severe medical condition that significantly increases cardiovascular disease as well as other chronic diseases [1‐3]. Hypertension risk factors [4‐8] include stress, harmful use of alcohol, salt consumption, low intake of fruits and vegetables, being overweight or obese, saturated fat and trans fats, tobacco use, low diet in vitamin D, lack of physical activity, family history, aged 65 years or over and co-existing diseases. Though the etiology of hypertension is complex, it was known as one of the risk factors was high serum uric acid (SUA). Most mechanisms [9] supported that uric acid (UA) induced acute vasoconstriction by activation of renin-angiotensin system (RAS), followed by uric acid uptake into vascular smooth muscle cells leading to cellular proliferation and secondary arteriolosclerosis that results in chronic salt-sensitive hypertension. Thus, increased serum uric acid increases the risk of developing hypertension. In addition to known and probable risk factors for hypertension, early life mal-nutrition may also affect hypertension. Though the mechanisms are unclear, it is speculated that some fetal growth regulation factors might be most vulnerable to nutrient deficiencies, specifically during fetal and early postnatal life, dependent on the window of exposure [10].

It was hypothesized that early developmental adaptions in response to malnutrition in early life, which are key determinants of short-term survival, have adverse cardiovascular outcomes [11, 12]. Historical famine exposure has provided a unique and natural opportunity to test the hypothesis. Previous famine studies [10, 13‐24] have provided pieces of evidence to support the association between famine exposure and increased risk of hypertension. Most studies [10, 13, 15, 17‐24] found that exposure to famine in early life increases the risk of BP/hypertension in adulthood. Furthermore, exposure to famine has more deleterious effects on adult health for women than men [14, 16]. However, other studies [15, 25, 26] did not find a significant association between famine exposure and hypertension. Therefore, the association between famine exposure in early life and the risk of BP/hypertension in later life needs to be further studied. Moreover, results also indicated the malnutrition in early life were more strongly correlated with hyperuricemia in late life [27, 28]. It is not completed understood association and interaction analysis between famine exposure and serum uric acid and hypertension in the mid-age and older adult.

Given the limitations of previous studies, our research analyzed data from the China Health and Retirement Longitudinal Study (CHARLS) Wave1 and aimed to investigate the individual and combined association between famine exposure and serum uric acid and hypertension after adjustment for confounding variables.

Table 1 shows the basic characteristics of participants. A total of 9368 individuals were enrolled in the study, 4366 (46.61%) and 5002 (53.39%) were male and female, respectively. Among males, 459 (10.51%) had been exposed to the Chinese famine during the fetal stage, whereas 1760 (40.31%) and 1645 (37.68%) had been exposed to the famine during childhood and adolescence/adult stage, respectively. The distribution of physical exercises habit did not demonstrate a significantly statistical difference among the four birth groups. On the other hand, the differences were observed in the distribution of age, BMI, education, marital status, living place, cigarette smoking, alcohol consumption status, eating habit, social events, history of accidental injury, hypertension, and SUA levels. Among females, 635 (12.69%) had been exposed to the Chinese famine during the fetal stage, whereas 1988 (39.74%) and 1569 (31.37%) had been exposed to the famine during childhood and adolescence/adult stage, respectively. Furthermore, the distribution of living place and history of accidental injury did not demonstrate significantly statistical differences among the four birth groups. On the other hand, the difference was observed in the distribution of age, BMI, education, marital status, cigarette smoking, alcohol consumption status, eating habit, social events, physical exercises habit, hypertension, and SUA levels.

Table 2 shows the characteristics of study participants categorized by SUA levels. Of the participants, 290 (6.64%) reported having HUA in the male and 234 (4.68%) in the female. In males, significant differences were observed in age, BMI, living place, cigarette smoking, alcohol consumption status, physical exercises habit, famine exposed stages, and hypertension groups (P < 0.05) between participants with and without HUA. Regarding the females, significant differences were observed in age, BMI, marital status, living place, cigarette smoking, famine exposed stages, and hypertension groups (P < 0.05) between participants with and without HUA.

Table 3 shows the characteristics of study participants categorized by blood pressure status. Of the participants, 1357 (31.08%) reported having hypertension in male and 1619 (32.37%) in the female. In males, significant differences were observed in age, BMI, education, marital status, living place, history of accidental injury, famine stages, and hypertension groups (P < 0.05) between participants with and without hypertension. In females, significant differences in distribution were observed between blood pressure status in the variables, including age, BMI, education, marital status, alcohol consumption status, famine stages, and SUA level groups.

Table 4 shows the separate associations of famine exposure, SUA levels with the prevalence of hypertension. Firstly, after controlling for confounding factors including age, education, marital status, living place, smoking status, alcohol consumption status, eating meals, social and leisure activities, the experience of a traumatic event, taking physical activity or exercise, BMI, and famine exposure in a multivariable logistic regression model four, higher odds of prevalence of hypertension in the total population were observed with increasing levels of SUA [high vs normal:1.73 (95% CI 1.44, 2.08) independently of famine stages only. When stratified by sex, the results of model four in both males and females were similar to those in the total population. Secondly, after controlling for confounding factors including age, education, marital status, living place, smoking status, alcohol consumption status, eating meals, social and leisure activities, the experience of a traumatic event, taking physical activity or exercise, BMI, and SUA levels in a multivariable logistic regression model four, higher odds of prevalence of hypertension in the total population were observed with famine exposed stages [fatal exposed group vs non-exposed group: 1.25 (95% CI 1.03, 1.52); childhood-exposed group vs non-exposed group:1.60 (95% CI 1.37, 1.87); adolescence/adult exposed group vs non-exposed group: 2.87 (95% CI 2.44, 3.37), P for trend < 0.001] independently of SUA levels only. When stratified by sex, the results of model four in both males and females were similar to those in the total population.

Table 5 shows the combined associations of SUA levels and famine exposure with the prevalence of hypertension. Compared with the combination of normal SUA level and no-exposed famine stage, all groups trended towards higher odds of prevalence of hypertension; Furthermore, in multivariable model one, the greatest increase in odds was observed for the adolescence/adult exposed stage and high SUA level combination (adolescence/adult exposed stage and HUA in total participants: OR 4.37; 95%CI 3.28,5.81). And similarly, in multivariable-adjusted model two, the highest odds of prevalence of hypertension were observed for the adolescence/adult exposed stage and HUA combination (adolescence/adult exposed stage and high SUA in total participants: OR 3.94; 95%CI 2.96, 5.26). Additionally, in multivariable-adjusted model three, the highest odds of prevalence of hypertension were observed for the adolescence/adult exposed stage and high SUA combination (adolescence/adult exposed stage and HUA in total participants: OR3.99; 95%CI 2.99, 5.32). In multivariable-adjusted model four, the highest odds of prevalence of hypertension were observed for the adolescence/adult exposed stage and HUA combination (adolescence/adult exposed stage and high SUA level in total participants: OR4.34; 95%CI 3.24, 5.81). Finally, combined associations of high SUA levels and famine exposure with the prevalence of hypertension were observed in the total participant (P-interaction < 0.001). When stratified by sex, the results of the model in both males and females were similar to those in the total population.

Our research aimed to explore the individual and combined association between famine exposure and serum uric acid and hypertension in mid-aged and older adults. Interestingly, our study found that the individuals exposed to famine in early life had an increased risk of hypertension in adult. After adjustment for observed confounders, including age, education, marital status, living place, smoking status, alcohol consumption status, eating meals, social and leisure activities, the experience of a traumatic event, taking physical activity or exercise, BMI, and SUA level, the associations still existed both in males and females. Additionally, the study showed that there were linear trends in the associations of SUA with hypertension. After adjustment for observed confounders, including age, education, marital status, living place, smoking status, alcohol consumption status, eating meals, social and leisure activities, the experience of a traumatic event, taking physical activity or exercise, BMI, and famine exposure, the associations still existed both in males and females. In general, our data support a strongly positive individual and combined association of famine exposure and SUA levels with hypertension in middle-aged and elderly Chinese. The outcomes of these individuals stratified by sex were examined. The results in both males and females were also similar to those in the total population.

The Chinese famine ranged from the late1950s to the early 1960s, caused over 30 million excess deaths in most areas [38]. Most studies have reported the associations of famine exposure during early life with hypertension in adults, but no consistent associations were observed. Therefore, this research attempted to examine the individual and combined association between famine exposure and serum uric acid and hypertension based on a population-based cross-sectional study from CHARLS. Our data support a strongly positive combined association of famine exposure and serum uric acid with hypertension in middle-aged and elderly Chinese. Both nutrition intervention for exposure to the famine in early life and serum uric acid reduction in later life may be required to substantially reduce the prevalence of hypertension.

As the worst famine, the survivors might be healthier than the weak members were kicked out, a common finding that is in line with Darwin’ s theory of survival of the fittest [39]. In this case, the participants exposed to famine in early life should decrease the risk of hypertension in adults. This was not observed in our research. When facing the later “rich” environment, the risk of hypertension may be increased. The outcomes are partly in line with previous studies. Although the Dutch famine and the Leningrad siege study [40‐42] found that early-life exposure to famine was not associated with hypertension, most studies [10, 13, 14, 16‐24] in China indicated that exposure to famine in early life increased the risk of hypertension. However, this association did not exist between the Chinese famine and hypertension risk in Chongqing [25]. Such discrepancies between those studies may be a result of methodological differences in definitions of famine exposure groups and the different sample selection effect. Additionally, these studies have been criticized for not being adjusted the effect of age. To control the potential age confounding, we categorized the famine exposure into four exposure groups [no-exposed stage (born between1963 and 1966), fetal exposed stage (born between1959 and 1962), childhood exposed stage (born between 1949 and 1958), adolescence/adult exposed stage (born between1921 and 1948)] based on the birth year and we also combined the no-exposure as the reference group to identify the effect of the fetal exposed stage, childhood exposed stage, adolescence/adult exposed stage. Our results suggested that early famine exposure was associated with an increased risk of hypertension. The sex difference of early life famine exposure and hypertension were common in several studies [16, 18]. Furthermore, exposure to famine during early life exerted more deleterious association on women than men. This could be explained by the fact the women may suffer more than men during the famine because of the dominance of a patriarchal mentality in China [43]. The main potential mechanisms of the relationship between famine exposure in early life and the increased risk of hypertension in later life were still not fully understood. Animal models [44, 45] have proved that undernutrition in early life could lead to hypertension in later life. In addition, epigenetic might play a part role in the relationship between famine exposure in early life and hypertension in adults [46, 47]. Though the potential mechanisms are unclear, it is speculated that some fetal growth regulation factors might be most vulnerable to nutrient deficiencies, specifically during fetal and early postnatal life, dependent on the window of exposure [10].

Although previous studies [48‐55] have estimated the association between serum uric acid level and blood pressure/hypertension. However, the results are not consistent. Y. Kansui, T. Ohtsubo, K. Goto, et al.[48]found that both systolic and diastolic blood pressures were significantly correlated with serum uric acid among Japanese male workers aged 18–64 years. Lyngdoh et al. [49] found that adiposity substantially decreased the association between SUA and BP in adults, and BP was independently associated with SUA in females. Kawamoto et al. [50] found that serum uric acid level significantly associated with both systolic blood pressure (SBP) and diastolic blood pressure (DBP) in females aged < 55 years but not in those aged ≥ 55 years. Irijanto et al. [53]found that community-dwelling Japanese men with a BMI ≥ 21.0 kg/m², serum uric acid level was positively correlated with SBP and DBP, but negatively associated with SBP and DBP in those with a BMI of ≥ 21.0 kg/m². Lin et al. [55] found that serum UA levels are significantly correlated to BP in Taiwanese adolescents aged 14–19 years. Khanum et al. [56] found that the relationship between elevated SUA level and incident hypertension was observed among individuals aged < 55 years, but not observed among participants aged ≥ 55 years. Cao et al. [57] found that the positive relationship between elevated SUA level and hypertension was proved in a Chinese population. Several longitudinal studies [58‐61] found that the serum uric acid level was positively related with the risk of incident hypertension independently. Similarly, other cross-sectional [62‐64] also found that hyperuricemia was significantly related with the risk of hypertension. The difference between those studies may due to the different confounding variables by controlling, the different populations, and different sampling methods selection. Several hypotheses partly explain the association between SUA level and high blood pressure/hypertension. One of the possible mechanism might be uric acid deposition on the blood vessels walls activates the renin-angiotensin system, suppress the liberate of carbon monoxide, enhance inflammation, and leads to vasoconstriction later [62]. Another possibility involving oxidative stress and endothelial dysfunction related with high SUA level may contribute to elevated blood pressure [65, 66].

There were so many studies that had explored the association analysis between famine exposure/ obesity parameters and BP/hypertension, and only two studies that explored the combined association between famine exposure and obesity parameters and hypertension, but no study was aimed to investigate the combined association of famine exposure and serum uric acid with hypertension after adjustment for confounding variables. Yu et al.[20] found that interactions between famine and obesity on hypertension prevalence risk were not observed. In contrast, Li et al. [13] reported that a stronger interaction between obesity and famine exposure concerning BP among individuals who were exposed to famine during fetal life and had a western dietary pattern in adults was observed. Two studies [27, 28] also found famine exposure was associated with an increased risk of hyperuricemia in adulthood. Interestingly, our data support a strongly positive combined association of famine exposure and serum uric acid with hypertension in middle-aged and elderly Chinese.

There were several limitations to the study. First, selection bias was to be considered: famine may weed out the frail members of the population and leave the healthier ones. Second, famine exposure for each individual was unknown. Third, not all families were equally affected by famine exposure. Fourth, the data was collected in 2011, and more recent studies are needed to identify the associations. However, the results provided large data that could be explored further in the combined association of famine exposure and serum uric acid with hypertension. Moreover, a significant strength of the study is the large sample of 9368 middle-aged and older Chinese. Another strength is the analytical method the controlled the potential confounders.

Our data support a positive individual and combined association of famine exposure and serum uric acid with hypertension in middle-aged and elderly Chinese. Both nutrition intervention for exposure to the famine in early life and serum uric acid reduction in later life may be required to substantially reduce the risk of hypertension.