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01.12.2014 | Original Article

Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in rice calli

verfasst von: Myunghwan Jung, Yun Ji Shin, Ju Kim, Seung-Bin Cha, Won-Jung Lee, Min-Kyoung Shin, Seung Won Shin, Moon-Sik Yang, Yong-Suk Jang, Tae-Ho Kwon, Han Sang Yoo

Erschienen in: Archives of Virology | Ausgabe 12/2014

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Abstract

Classical swine fever (CSF), caused by the CSF virus (CSFV), is a highly contagious disease in pigs. In Korea, vaccination using a live-attenuated strain (LOM strain) has been used to control the disease. However, parenteral vaccination using a live-attenuated strain still faces a number of problems related to storage, cost, injection stress, and differentiation of CSFV infected and vaccinated pigs. Therefore, two kinds of new candidates for oral vaccination have been developed based on the translation of the E2 gene of the SW03 strain, which was isolated from an outbreak of CSF in 2002 in Korea, in transgenic rice calli (TRCs) from Oriza sativa L. cv. Dongjin to express a recombinant E2 protein (rE2-TRCs). The expression of the recombinant E2 protein (rE2) in rE2-TRCs was confirmed using Northern blot, SDS-PAGE, and Western blot analysis. Immune responses to the rE2-TRC in mice and pigs were investigated after oral administration. The administration of rE2-TRCs increased E2-specific antibodies titers and antibody-secreting cells when compared to animals receiving the vector alone (p < 0.05 and p < 0.01). In addition, mice receiving rE2-TRCs had a higher level of CD8+ lymphocytes and Th1 cytokine immune responses to purified rE2 (prE2) in vitro than the controls (p < 0.05 and p < 0.01). Pigs receiving rE2-TRCs also showed an increase in IL-8, CCL2, and the CD8+ subpopulation in response to stimulation with prE2. These results suggest that oral administration of rE2-TRCs can induce E2-specific immune responses.

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Titel: Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in rice calli
verfasst von: Myunghwan Jung
Yun Ji Shin
Ju Kim
Seung-Bin Cha
Won-Jung Lee
Min-Kyoung Shin
Seung Won Shin
Moon-Sik Yang
Yong-Suk Jang
Tae-Ho Kwon
Han Sang Yoo
Publikationsdatum: 01.12.2014
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 12/2014
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-014-2182-4

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Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in rice calli

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