Erschienen in:
01.11.2005 | Original Article
Induction of Th1-type immunity and tumor protection with a prostate-specific antigen DNA vaccine
verfasst von:
Deborah J. Marshall, Lani R. San Mateo, Kelly A. Rudnick, Stephen G. McCarthy, Michael C. Harris, Christine McCauley, Allen Schantz, Dong Geng, Pam Cawood, Linda A. Snyder
Erschienen in:
Cancer Immunology, Immunotherapy
|
Ausgabe 11/2005
Einloggen, um Zugang zu erhalten
Abstract
Prostate specific antigen (PSA) is a serum marker that is widely used in the detection and monitoring of prostate cancer. Though PSA is a self-antigen, T cell responses to PSA epitopes have been detected in healthy men and prostate cancer patients, suggesting it may be used as a target for active immunotherapy of prostate cancer. A PSA DNA vaccine (pPSA) was evaluated in mice and monkeys for its ability to induce antigen-specific immune responses. Mice immunized intradermally with pPSA demonstrated strong PSA-specific humoral and cellular immunity. The anti-PSA immune responses were skewed toward Th1, as shown by high IFNγ and IL-2 production. The immune response was sufficient to protect mice from challenge with PSA-expressing tumor cells. Tumor protection was durable in the absence of additional vaccination, as demonstrated by protection of vaccinated mice from tumor rechallenge. Furthermore, pPSA vaccination induced PSA-specific antibody titers in male cynomolgus monkeys, which express a closely related PSA gene. These results demonstrate that vaccination with pPSA may be able to break tolerance and can induce an immune response that mediates tumor protection.