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Clinical Oral Investigations

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28.01.2019 | Original Article

Influence of buffy coat–derived putative endothelial progenitor cells on tumor growth and neovascularization in oral squamous cell carcinoma xenografts

verfasst von: Marius Otto, Sebastian Blatt, Andreas Pabst, Robert Mandic, Johanna Schwarz, Andreas Neff, Thomas Ziebart

Erschienen in: Clinical Oral Investigations | Ausgabe 10/2019

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Abstract

Objectives

The aim of this murine in vivo study was to investigate whether buffy coat–derived putative endothelial progenitor cells (BC^EPC) alter tumor growth and neovascularization in oral squamous cell carcinomas (OSCC).

Materials and methods

A murine xenograft model using the PCI-13 oral cancer cell line was deployed of which n = 24 animals received 2 × 10⁶ BC^EPC by transfusion whereas the control group (n = 24) received NaCl (0.9%) instead. Tumor size, volume, and capillary density were determined by sonography and measurement with a caliper. Immunohistochemical analysis was carried out with antibodies specific for Cytokeratins, Flt-4, Podoplanin, and Vimentin.

Results

In the experimental group, systemic application of BC^EPC significantly increased tumor volume to 362.49% (p = 0.0012) and weight to 352.38% (p = 0.0018) as well as vascular densities to 162.15% (p = 0.0021) compared with control tumors. In addition, BC^EPC-treated xenografts exhibited higher Cytokeratin expression levels by a factor of 1.47 (p = 0.0417), Podoplanin by a factor of 3.3 (p = 0.0020) and Vimentin by a factor of 2.5 (p = 0.0001), respectively.

Conclusions

Immunohistochemical investigations support the notion that BC^EPC transfusion influences neovascularization and lymphatic vessel density, thereby possibly promoting tumor progression. Future studies, which will include gene expression analysis, should help to define the possible role of BC^EPC during OSCC progression in more detail.

Clinical relevance

Endothelial progenitor cells (EPCs) could serve as a target structure for the treatment of OSCC and possibly other solid tumors.

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Titel: Influence of buffy coat–derived putative endothelial progenitor cells on tumor growth and neovascularization in oral squamous cell carcinoma xenografts
verfasst von: Marius Otto
Sebastian Blatt
Andreas Pabst
Robert Mandic
Johanna Schwarz
Andreas Neff
Thomas Ziebart
Publikationsdatum: 28.01.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 10/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-02806-2

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Influence of buffy coat–derived putative endothelial progenitor cells on tumor growth and neovascularization in oral squamous cell carcinoma xenografts

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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