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Clinical Oral Investigations

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23.09.2015 | Original Article

Influence of CAD/CAM zirconia for implant-abutment manufacturing on gingival fibroblasts and oral keratinocytes

verfasst von: A. M. Pabst, C. Walter, A. Bell, M. Weyhrauch, I. Schmidtmann, H. Scheller, K. M. Lehmann

Erschienen in: Clinical Oral Investigations | Ausgabe 5/2016

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Abstract

Objectives

This study investigated the influence of three CAD/CAM zirconia ceramics for implant-abutment manufacturing on cell viability, migration ability, and cytotoxicity of human gingival fibroblasts (HGF) and oral keratinocytes (HOK) in vitro.

Materials and methods

HGF and HOK were cultured on zirconia ceramic disks (VITA In-Ceram YZ, Ivoclar IPS e.max ZirCAD, Sirona inCoris ZI) and on control disks made of tissue culture polystyrene. Cell viability was analyzed by a MTT assay. Migration ability was detected by a scratch assay. A ToxiLight assay was used to analyze the influence of the tested zirconia ceramics on adenylate kinase (ADK) release and cytotoxicity.

Results

At MTT assay, HGF showed an increased cell viability compared to the control after 9 and 12 days for all ceramics (p each ≤0.0002) while HOK demonstrated a decreased cell viability after 9 and 12 days for all ceramics (p each ≤0.0003). At scratch assay, HGF exhibited for all ceramics decreased relative distances of the scratch wound compared to the control from 24 to 48 h (p each <0.0001) with exception of VITA In-Ceram YZ after 48 h. HOK showed increased distances compared to the control for all ceramics after 48 h (p each <0.0001). At ToxiLight assay, a minimal cytotoxicity of the tested materials could be detected.

Conclusions

Overall, significant influences of the investigated CAD/CAM zirconia ceramics on HGF and HOK could be shown.

Clinical relevance

The analyzed zirconia ceramics could influence oral soft-tissue cells that might affect the esthetic outcome after implant placement using CAD/CAM zirconia abutments.

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Titel: Influence of CAD/CAM zirconia for implant-abutment manufacturing on gingival fibroblasts and oral keratinocytes
verfasst von: A. M. Pabst
C. Walter
A. Bell
M. Weyhrauch
I. Schmidtmann
H. Scheller
K. M. Lehmann
Publikationsdatum: 23.09.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 5/2016
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1598-x

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Influence of CAD/CAM zirconia for implant-abutment manufacturing on gingival fibroblasts and oral keratinocytes

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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Springer Medizin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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