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Erschienen in: Inflammation Research 5/2020

21.03.2020 | Original Research Paper

Influence of estradiol treatment on bone marrow cell differentiation in collagenase-induced arthritis

verfasst von: Petya Ganova, Ralitsa Zhivkova, Anton Kolarov, Nina Ivanovska

Erschienen in: Inflammation Research | Ausgabe 5/2020

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Abstract

Objective and design

Estrogen is one of the important regulators of the balance between bone formation and bone resorption that can modulate the levels and activity of certain growth factors and cytokines. In this study, we investigated the effect of 17β-estradiol (ED) on bone marrow (BM) cell differentiation in vivo and ex vivo in a mouse model of collagenase-induced osteoarthritis (CIOA).

Subject

ICR (CD-2) female mice were used in present experiments (total number = 75) and bone marrow cells were used for in vitro studies.

Treatment

Mice were orally fed under different schemes with 17β-estradiol at a dose of 2 μg or 4 μg for 30 days.

Methods

The effect of estradiol was estimated by histopathological, flow cytometry, and ELISA assays. Statistical differences were determined by one-way ANOVA.

Results

Estradiol treatment ameliorated cartilage destruction and osteophyte formation if started from day 0 of CIOA induction, attended with a decrease of uterine and ovarian weights. Long time treatment lowered the percentage of megakaryocyte/platelet (CD62P+) populations and osteoclast (RANK+) populations in BM. Cells obtained from estradiol-treated CIOA mice showed inhibited capacity to differentiate into RANK+ and mesenchymal cells under osteoclastogenic conditions in vitro. Estrogen decreased serum IL-6 levels.

Conclusion

Results indicate a potential protective role for estrogen against the development of OA.
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Metadaten
Titel
Influence of estradiol treatment on bone marrow cell differentiation in collagenase-induced arthritis
verfasst von
Petya Ganova
Ralitsa Zhivkova
Anton Kolarov
Nina Ivanovska
Publikationsdatum
21.03.2020
Verlag
Springer International Publishing
Erschienen in
Inflammation Research / Ausgabe 5/2020
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-020-01338-w

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