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Transplantation of ovarian tissue is a valuable method to rescue mouse strains with fertility problems and to revitalize archived strains. The purpose of this study was to investigate the effect of (i) different sizes of transplanted ovary pieces on reproductive outcome, (ii) use of immunodeficient recipients to overcome the limitation of histocompatibility, and (iii) to compare different protocols for cryopreservation of ovarian tissue.
Halves, quarters, and eights of mouse ovaries were transplanted. Half ovaries from B6 donors were transferred into immunodeficient mice. Halves of ovaries were frozen according to four different protocols, thawed and transferred.
Pregnancy rate after transplantation of ovarian tissue was high (90–100%) independent of the transplant size. Although, the average litter size was significantly lower for recipients of quarters and eights (4.4 and 4.6 vs. 6.5), the total number of offspring produced per donor ovary was higher compared with recipients of halves. Pregnancy rate of immunodeficient recipients was 40% (mean 4.7 offspring per litter). All four cryopreservation protocols used were able to preserve functionality of the ovarian tissue.
Transplantation of ovarian tissue smaller than halves resulted in reduced litter sizes. The distribution of ovarian tissue of one donor female to 4 or 8 recipients will therefore yield in a higher total number of offspring in a certain time period. The use of immunodeficient recipients is an option for non-histocompatible donors. Cryopreservation of ovarian tissue is generally feasible but the function of frozen-thawed ovary halves after transplantation differs depending on the freezing protocol used.
Russell WL, Hurst JG. Pure strain mice born to hybrid mothers following ovarian transplantation. PNAS. 1945;31(9):267–73. PubMed
Cox SL, Shaw J, Jenkin G. Transplantation of cryopreserved fetal ovarian tissue to adult recipients in mice. J Reprod Fertil. 1996;107:315–22. PubMed
Sztein J, Sweet H, Farley J, Mobraaten L. Cryopreservation and orthotopic transplantation of mouse ovaries: new approach in gamete banking. Biol Reprod. 1998;58:1071–4. PubMed
Sztein J, McGregor TE, Bedigian HJ, Mobraaten LE. Transgenic mouse strain rescue by frozen ovaries. Lab Anim Sci. 1999;49(1):99–100. PubMed
Sztein J, O’Brien MJ, Farley JS, Mobraaten LE, Eppig JJ. Rescue of oocytes from antral follicles of cryopreserved mouse ovaries: competence to undergo maturation, embryogenesis, and development to term. Hum Reprod. 2000;15(3):567–71. PubMed
Candy CJ, Wood MJ, Whittingham DG. Restoration of a normal reproductive lifespan after grafting of cryopreserved mouse ovaries. Hum Reprod. 2000;15(6):1300–4. PubMed
Snow M, Cox S-L, Jenkin G, Shaw J. Fertility of mice following receipt of ovaries slow cooled in dimethylsufoxide or ethylene glycol is largely independent of crypreservation equilibration time and temperature. Reprod Fertil Dev. 2003;15:407–14. PubMed
Liu L, Wood GA, Morikawa L, Ayearst R, Fleming C, McKerlie C. Restoration of fertility by orthotopic transplantation of frozen adult mouse ovaries. Hum Reprod. 2008;23:122–8. PubMed
Takahashi E, Miyoshi I, Nagasu T. Rescue of a transgenic mouse line by transplantation of a frozen-thawed ovary obtained postmortem. Contemp Top Lab Anim Sci. 2001;40(4):28–31. PubMed
Kamoshita K, Okamoto N, Nakajima M, Haino T, Sugimoto K, Okamoto A, et al. Investigation of in vitro parameters and fertility of mouse ovary after storage at an optimal temperature and duration for transportation. Hum Reprod. 2016;31(4):774–81. PubMed
Gunasena KT, Villines PM, Critser ES, Critser JK. Live births after autologous transplant of cryopreserved mouse ovaries. Hum Reprod. 1997a;12(1):101–6. PubMed
Gunasena KT, Lakey JRT, Villines PM, Critser ES, Critser JK. Allogeneic and xenogeneic transplantation of cryopreserved ovarian tissue to athymic mice. Biol Reprod. 1997b;57:226–31. PubMed
Migishima F, Suzuki-Migishima R, Song A-Y, Kuramochi T, Azuma S, Nishijima M, et al. Successful cryopreservation of mouse ovaries by vitrification. Biol Reprod. 2003;68:881–7. PubMed
Wang X, Catt S, Pangestu M, Temple-Smith P. Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice. Reproduction. 2009;138:527–35. PubMed
Agca C, Lucy MC, Agca Y. Gene expression profile of rat ovarian tissue following xenotransplantation into immune-deficient mice. Reproduction. 2009;137(6):957–67. PubMed
Weissman A, Gotlieb L, Colgan T, Jurisicova A, Greenblatt EM, Casper RF. Preliminary experience with subcutaneous human ovarian cortex transplantation in the NOD-SCID mouse. Biol Reprod. 1999;60:1462–7. PubMed
Kim SS, Kang HG, Kim NH, Lee HC, Lee HH. Assessment of the integrity of human oocytes retrieved from cryopreserved ovarian tissue after xenotransplantation. Hum Reprod. 2005;20(9):2502–8. PubMed
Dolmans M-M, Yuan WY, Camboni A, Torre A, Langendonckt AV, Martinez-Madrid B, et al. Development of antral follicles after xenografting of isolated small human preantral follicles. RBM Online. 2008;16(5):705–11. PubMed
Baird DT, Webb R, Campbell BK, Harkness LM, Gosden RG. Long-term ovarian function in sheep after ovariectomy and transplantation of autografts stored at -196 C. Endocrinology. 1999;140(1):462–71. PubMed
Bosch P, Hernandez-Fonseca HJ, Miller DM, Wininger JD, Massey JB, Lamb SV, et al. Development of antral follicles in cryopreserved cat ovarian tissue transplanted to immunodeficient mice. Theriogenology. 2004;61:581–94. PubMed
Demirel MA, Acar DB, Ekim B, Celikkan FT, Alkan KK, Salar S, et al. The evaluation of xenotransplantation of feline ovarian tissue vitrified by needle immersed vitrification technique into male immunodeficient mice. Cell Tissue Bank. 2018;19(1):133–47. PubMed
Fernandes DP, Pimentel MML, Santos FAD, Praxedes ÉA, Brito PD, Lima MA, et al. Hematological and biochemical profile of BALB/c nude and C57BL/6 SCID female mice after ovarian xenograft. An Acad Bras Cienc. 2018;90(4):3941–8. PubMed
Cleary M, Shaw JM, Jenkin G, Trounson AO. Influence of hormone environment and donor age on cryopreserved common wombat (Vombatus ursinus) ovarian tissue xenografted into nude mice. Reprod Fertil Dev. 2004;16(7):699–707. PubMed
Gunasena KT, Lakey JRT, Villines PM, Bush M, Raath C, Critser ES, et al. Antral follicles develop in xenografted cryopreserved african elephant (Loxodonta africana) ovarian tissue. Anim Reprod Sci. 1998;53:265–75. PubMed
Wakasa I, Hayashi M, Abe Y, Suzuki H. Distribution of follicles in canine ovarian tissues and xenotransplantation of cryopreserved ovarian tissues with even distribution of follicles. Reprod Domest Anim. 2017;52(Suppl 2):219–23. PubMed
Wiedemann C, Hribal R, Ringleb J, Bertelsen MF, Rasmusen K, Andersen CY, et al. Preservation of primordial follicles from lions by slow freezing and xenotransplantation of ovarian cortex into an immunodeficient mouse. Reprod Domest Anim. 2012;47(Suppl. 6):300–4. PubMed
Suzuki N, Yoshioka N, Takae S, Sugishita Y, Tamura M, Hashimoto S, et al. Successful fertility preservation following ovarian tissue vitrification in patients with primary ovarian insufficiency. Hum Reprod. 2015;30:608–15. PubMed
Rodriguez-Wallberg KA, Karlström PO, Rezapour M, Castellanos E, Hreinsson J, Rasmussen C, et al. Full-term newborn after repeated ovarian tissue transplants in a patient treated for Ewing sarcoma by sterilizing pelvic irradiation and chemotherapy. Acta Obstet Gynecol Scand. 2015;94:324–8. PubMedPubMedCentral
Demeestere I, Simon P, Dedeken L, Moffa F, Tsépélidis S, Brachet C, et al. Live birth after autograft of ovarian tissue cryopreserved during childhood. Hum Reprod. 2015;30(9):2107–9. PubMed
Nakao K, Nakagata N, Katsuki M. Simple and efficient vitrification procedure for cryopreservation of mouse embryos. Exp Anim. 1997;46(3):231–4. PubMed
Candy CJ, Wood MJ, Whittingham DG. Effect of cryoprotectants on the survival of follicles in frozen mouse ovaries. J Reprod Fertil. 1997;110:11–9. PubMed
Aerts JMJ, De Clercq JBP, Andries S, Leroy JLMR, Van Aelst S, Bols PEJ. Follicle survival and growth to antral stages in short-term murine ovarian cortical transplants after Cryologic solid surface vitrification or slow-rate freezing. Cryobiology. 2008;57:163–9. PubMed
Mahler CM, Berard M, Feinstein R, et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units. Lab Anim. 2014;48:178–92.
Dinnyes A, Dai Y, Jiang S, Yang X. High developmental rates of vitrified bovine oocytes following parthenogenetic activation, in vitro fertilization, and somatic cell nuclear transfer. Biol Reprod. 2000;63:513–8. PubMed
Gosden RG, Baird DT, Wade JC, Webb R. Restoration of fertility to oophorectomized sheep by ovarian autografts stored at -196 degrees C. Hum Reprod. 1994;9:597–603. PubMed
Nugent D, Newton H, Gallivan L, Gosden RG. Protective effect of vitamin E on ischaemia-reperfusion injury in ovarian grafts. J Reprod Fertil. 1998;114:341–6. PubMed
Dolmans MM, Martinez-Madrid B, Gadisseux E, Guiot Y, YuanWY TA, Camboni A, et al. Short-term transplantation of isolated human ovarian follicles and cortical tissue into nude mice. Reproduction. 2007;134:253–62. PubMed
Silber S, Kagawa N, Kuwayama M, Gosden R. Duration of fertility after fresh and frozen ovary transplantation. Fertil Steril. 2010;94:2191–6. PubMed
Silber SJ. Ovary cryopreservation and transplantation for fertility preservation. Mol Hum Reprod. 2012;18:59–67. PubMed
von Schonfeldt V, Chandolia R, Ochsenkuhn R, Nieschlag E, Kiesel L, Sonntag B. FSH prevents depletion of the resting follicle pool by promoting follicular number and morphology in fresh and cryopreserved primate ovarian tissues following xenografting. Reprod Biol Endocrinol. 2012;10:98.
- Influence of graft size, histocompatibility,and cryopreservation on reproductive outcome following ovary transplantation in mice
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