This multi-country prospective study of infants aged <1 year aims to assess the frequency of influenza virus and respiratory syncytial virus (RSV) infections associated with hospitalizations, to describe clinical features and antibody response to infection, and to examine predictors of very severe disease requiring intensive care.
We are enrolling a hospital-based cohort and a sample of non-ill infants in four countries (Albania, Jordan, Nicaragua, and the Philippines) using a common protocol. We are currently starting year 2 of a 2- to 3-year study and will enroll approximately 3,000 infants hospitalized for any acute illness (respiratory or non-respiratory) during periods of local influenza and/or RSV circulation. After informed consent and within 24 h of admission, we collect blood and respiratory specimens and conduct an interview to assess socio-demographic characteristics, medical history, and symptoms of acute illness (onset ≤10 days). Vital signs, interventions, and medications are documented daily through medical record abstraction. A follow-up health assessment and collection of convalescent blood occurs 3-5 weeks after enrollment. Influenza and RSV infection is confirmed by singleplex real time reverse transcriptase polymerase chain reaction (rRT-PCR) assays. Serologic conversion will be assessed comparing acute and convalescent sera using hemagglutination inhibition assay for influenza antibodies and enzyme-linked immunosorbent assay (ELISA) for RSV. Concurrent with hospital-based enrollment, respiratory specimens are also being collected (and tested by rRT-PCR) from approximately 1,400 non-ill infants aged <1 year during routine medical or preventive care.
The Influenza and RSV in Infants Study (IRIS) promises to expand our knowledge of the frequency, clinical features, and antibody profiles of serious influenza and RSV disease among infants aged <1 year, quantify the proportion of infections that may be missed by traditional surveillance, and inform decisions about the potential value of existing and new vaccines and other prevention and treatment strategies.
Leelarasamee A, Chupaprawan C, Chenchittikul M, Udompanthurat S. Etiologies of acute undifferentiated febrile illness in Thailand. J Med Assoc Thai. 2004;87(5):464–72. PubMed
Hall CB, Simoes EA, Anderson LJ. Clinical and epidemiologic features of respiratory syncytial virus. Curr Top Microbiol Immunol. 2013;372:39–57. PubMed
La Via WV, Grant SW, Stutman HR, Marks MI. Clinical profile of pediatric patients hospitalized with respiratory syncytial virus infection. Clin Pediatr (Phila). 1993;32(8):450–4. CrossRef
Thompson MG, Gaglani MJ, Naleway A, Thaker S, Ball S. Changes in Self-Rated Health and Subjective Social Status over Time in a Cohort of Healthcare Personnel. J Health Psychol. 2014;19(9):1185–96.
Network WHOGIS. Serological diagnosis of influenza by haemagglutination inhibition testing. In: Manual for the laboratory diagnosis and virological surveillance of influenza. Geneva: WHO; 2011.
Hulley SBCS, Browner WS, Grady D, Newman TB. Designing clinical research: an epidemiologic approach. Philadelphia: Lippincott Williams & Wilkins; 2013.
Hirve S, Krishnan A, Dawood FS, et al. Incidence of influenza-associated hospitalization in rural communities in western and northern India, 2010-2012: A multi-site population-based study. J Infect. 2015;70(2):160–70. doi: 10.1016/j.jinf.2014.08.015. Epub 2014 Sep 9.
Wood JM, Newman RW, Ploss K. The use of correlates of immunity in European Union licensing of influenza vaccines. Dev Biol (Basel). 2003;115:9–16.
Thompson MG, Gaglani MJ, Naleway AL, et al. Reduced serologic sensitivity to influenza A virus illness among inactivated influenza vaccinees. Vaccine. 2016;34(30):3443–6. doi: 10.1016/j.vaccine.2016.04.085. Epub 2016 May 16.
Munywoki PK, Koech DC, Agoti CN, Kibirige N, Kipkoech J, Cane PA, Medley GF, Nokes DJ. Influence of age, severity of infection, and co-infection on he duration of respiratory syncytial virus (RSV) shedding Epidemiol. Infect. 2015;143:804–812. doi: 10.1017/S0950268814001393.
- Influenza and respiratory syncytial virus in infants study (IRIS) of hospitalized and non-ill infants aged <1 year in four countries: study design and methods
Mark G. Thompson
Danielle R. Hunt
Ali K. Arbaji
Veronica L. Tallo
Holly M. Biggs
Ilham Abu Khader
Marilla G. Lucero
Basima I. Marar
Eric A. F. Simões
Min Z. Levine
Jennifer K. Meece
Tareq M. Al-Sanouri
Joanne N. de Jesus
Natalie J. Thornburg
Susan I. Gerber
on behalf of IRIS Network
- BioMed Central
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