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13.08.2018 | Laboratory Investigation

Inhibition of glioma growth by a GOLPH3 siRNA-loaded cationic liposomes

verfasst von: Zixuan Yuan, Liang Zhao, Yafei Zhang, Shun Li, Bomin Pan, Lei Hua, Zhen Wang, Chengkun Ye, Jun Lu, Rutong Yu, Hongmei Liu

Erschienen in: Journal of Neuro-Oncology | Ausgabe 2/2018

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Abstract

Purpose

GOLPH3 has been shown to be involved in glioma proliferation. In this study, we aimed to demonstrate that GOLPH3 can serve as a target for glioma gene therapy.

Methods

During the experiment, cationic liposomes with angiopep-2 (A2-CL) were used to deliver siGOLPH3 crossing the blood–brain barrier and reaching the glioma.

Results

At the cellular level, the A2-CL/siGOLPH3 could silence GOLPH3 and then effectively inhibited the proliferation of cells. In vivo experiments, using U87-GFP-Luci-bearing BALB/c mouse models, we demonstrated that A2-CL could deliver GOLPH3-siRNA specifically to glioma and effectively inhibit glioma growth.

Conclusions

This study shows that GOLPH3 has great potential as a target for the gene therapy of glioma and is of great value in precise medical applications.

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Titel: Inhibition of glioma growth by a GOLPH3 siRNA-loaded cationic liposomes
verfasst von: Zixuan Yuan
Liang Zhao
Yafei Zhang
Shun Li
Bomin Pan
Lei Hua
Zhen Wang
Chengkun Ye
Jun Lu
Rutong Yu
Hongmei Liu
Publikationsdatum: 13.08.2018
Verlag: Springer US
Erschienen in: Journal of Neuro-Oncology / Ausgabe 2/2018
Print ISSN: 0167-594X
Elektronische ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-018-2966-6

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Inhibition of glioma growth by a GOLPH3 siRNA-loaded cationic liposomes

Abstract

Purpose

Methods

Results

Conclusions

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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