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Archives of Virology

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01.01.2012 | Brief Report

Inhibition of Lassa virus and Ebola virus infection in host cells treated with the kinase inhibitors genistein and tyrphostin

verfasst von: Andrey A. Kolokoltsov, Shramika Adhikary, Jennifer Garver, Lela Johnson, Robert A. Davey, Eric M. Vela

Erschienen in: Archives of Virology | Ausgabe 1/2012

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Abstract

Arenaviruses and filoviruses are capable of causing hemorrhagic fever syndrome in humans. Limited therapeutic and/or prophylactic options are available for humans suffering from viral hemorrhagic fever. In this report, we demonstrate that pre-treatment of host cells with the kinase inhibitors genistein and tyrphostin AG1478 leads to inhibition of infection or transduction in cells infected with Ebola virus, Marburg virus, and Lassa virus. In all, the results demonstrate that a kinase inhibitor cocktail consisting of genistein and tyrphostin AG1478 is a broad-spectrum antiviral that may be used as a therapeutic or prophylactic against arenavirus and filovirus hemorrhagic fever.

Towner J, Sealy T, Ksiazek TG, Nichol ST (2007) High-throughput molecular detection of hemorrhagic fever virus threats with applications for outbreak settings. J Infect Dis 196:S205–S212PubMedCrossRef

Hensley LE, Mulangu S, Asiedu C, Johnson J, Honko AN, Stanley D, Fabozzi G, Nichol ST, Ksiazek TG, Rollin PE, Wahl-Jensen V, Bailey M, Jahrling PB, Roederer M, Koup RA, Sullivan NJ (2010) Demonstration of cross-protective vaccine immunity against an emerging pathogenic Ebolavirus species. PLoS Pathog 6:e1000904PubMedCrossRef

McCormick JB, King I, Webb P, Johnson K, O’Sullivan R, Smith E, Tripple S, Tong T (1986) Lassa fever: effective therapy with Ribavirin. N Engl J Med 314:20–26PubMedCrossRef

Akula S, Hurley D, Wixon R, Wang C, Chase C (2002) Effect of genistein on replication of bovine herpes virus type 1. Am J Veter Res 63:1124–1128CrossRef

Andres A, Donovan SM, Kuhlenschmidt TB, Kuhlenschmidt MS (2007) Isoflavones at concentrations present in soy infant formula inhibit Rotavirus infection in vitro. J Nutr 137(9):2068–2073

Lecot S, Belouzard S, Dubuisson J, Rouille Y (2005) Bovine viral diarrhea virus entry is dependent on clathrin-mediated endocytosis. J Virol 79:10826–10829PubMedCrossRef

Yura Y, Yoshida H, Sato M (1993) Inhibition of herpes simplex virus replication by genistein, an inhibitor of protein-tyrosine kinase. Arch Virol 132:451–461PubMedCrossRef

Vela EM, Colpitts T, Zhang L, Davey R, Aronson J (2008) Pichindé virus is trafficked through a dynamin 2 endocytic pathway that is dependent on cellular Rab5- and Rab7-mediated endosomes. Arch Virol 153:1391–1396PubMedCrossRef

Vela EM, Knostman KA, Mott JM, Warren RL, Garver JN, Vela LJ, Stammen RL (2010) Genistein, a general kinase inhibitor, as a potential antiviral for arenaviral hemorrhagic fever as described in the Pirital virus-Syrian golden hamster model. Antivir Res 87:318–328PubMedCrossRef

10.

Sbrana E, Mateo RI, Xiao S-Y, Popov VL, Newman PC, Tesh RB (2006) Clinical laboratory, virologic, and pathologic changes in hamsters experimentally infected with Pirital virus (Arenaviridae): a rodent model of Lassa fever. Am J Trop Med Hyg 74:1096–1102PubMed

11.

Vela E, Knostman K, Warren R, Garver J, Stammen R (2010) The disease progression associated with Pirital virus infection in the Syrian golden hamster. J Infect Dis Immun 2:15–23

12.

Xiao S-Y, Zhang H, Yang Y, Tesh RB (2001) Pirital virus (Arenaviridae) infection in the Syrian golden hamster, Mesocricetus auratus: a new animal model for arenaviral hemorrhagic fever. Am J Trop Med Hyg 64:111–118PubMed

13.

Han Y, Caday CG, Nanda A, Cavenee WK, Huang H-JS (1996) Tyrphostin AG 1478 preferentially inhibits human glioma cells expressing truncated rather than wild-type epidermal growth factor receptors. Cancer Res 56:3859–3861PubMed

14.

Vela EM, Bowick GC, Herzog NK, Aronson JF (2008) Genistein treatment of cells inhibits arenavirus infection. Antivir Res 77:153–156PubMedCrossRef

15.

Smit JM, Bittman R, Wilschut J (1999) Low-pH-dependent fusion of Sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes. J Virol 73:8476–8484PubMed

16.

Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:2002–2007CrossRef

17.

Bowick G, Fennewald S, Scott E, Zhang L, Elsom B, Aronson J, Spratt H, Luxon B, Gorenstein D, Herzog N (2007) Identification of differentially activated cell-signalling networks associated with Pichinde virus pathogenesis by using systems kinomics. J Virol 81:1923–1933PubMedCrossRef

18.

Radoshitzky S, Abraham J, Spiropoulou C, Kuhn J, Nguyen D, Wenhui L, Nagel J, Schmidt P, Nunberg J, Andrews N, Farzan M, Choe H (2007) Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses. Nature 446:92–96PubMedCrossRef

19.

Martinez M, Cordo S, Candurra N (2007) Characterization of Junin arenavirus cell entry. J Gen Virol 88:1776–1784PubMedCrossRef

20.

Vela E, Zhang L, Colpitts T, Davey R, Aronson J (2007) Arenavirus entry occurs through a cholesterol-dependent, non-caveolar, clathrin-mediated endocytic mechanism. Virology 369:1–11PubMedCrossRef

21.

Castilla V, Merisch S (1996) Low pH-induced fusion of Vero cells infected with Junin virus. Arch Virol 141:1307–1317PubMedCrossRef

22.

Di Simone C, Zandonatti M, Buchmeier M (1994) Acidic pH triggers LCMV membrane fusion activity and conformational change in the glycoprotein spike. Virology 198:455–465PubMedCrossRef

23.

Di Simone C, Buchmeier M (1995) Kinetics and pH dependence of acid-induced structural changes in the lymphoytic choriomeningitis virus glycoprotein complex. Virology 209:3–9PubMedCrossRef

24.

Benmerah A, Bayrou M, Cerf-Bensussan N, Dautry-Varsat A (1999) Inhibition of clathrin-coated pit assembly by an Eps15 mutant. J Cell Sci 112:1303–1311PubMed

25.

Conner S, Schmid S (2003) Regulated portasl of entry into the cell. Nature 422:37–44PubMedCrossRef

26.

McNiven M, Cao H, Pitts K, Yoon Y (2000) The dynamin family of mechanoenzymes: pinching in new places. TRENDS Biochem Sci 25:115–120PubMedCrossRef

27.

Ang F, Wong A, Ng M, Chu J (2010) Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus. Virol J 7:24PubMedCrossRef

28.

Bhattacharyya S, Warfield KL, Ruthel G, Bavari S, Aman MJ, Hope TJ (2010) Ebola virus uses clathrin-mediated endocytosis as an entry pathway. Virology 401:18–28PubMedCrossRef

29.

Jin M, Park J, Lee S, Park B, Shin J, Song KJ, Ahn TI, Hwang SY, Ahn BY, Ahn K (2002) Hantaan virus enters cells by clathrin-dependent receptor-mediated endocytosis. Virology 294:60–69PubMedCrossRef

30.

Simmons G, Rennekamp AJ, Chai N, Vandenberghe LH, Riley JL, Bates P (2003) Folate receptor alpha and Caveolae are not required for Ebola virus glycoprotein-mediated viral infection. J Virol 77:13433–13438PubMedCrossRef

Titel: Inhibition of Lassa virus and Ebola virus infection in host cells treated with the kinase inhibitors genistein and tyrphostin
verfasst von: Andrey A. Kolokoltsov
Shramika Adhikary
Jennifer Garver
Lela Johnson
Robert A. Davey
Eric M. Vela
Publikationsdatum: 01.01.2012
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 1/2012
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-1115-8

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Inhibition of Lassa virus and Ebola virus infection in host cells treated with the kinase inhibitors genistein and tyrphostin

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