Erschienen in:
01.02.2007 | ORIGINAL CONTRIBUTION
Inhibition of phospholipase A2 activity by conjugated linoleic acids in human macrophages
verfasst von:
Ewa Stachowska, Violetta Dziedziejko, Krzysztof Safranow, Izabela Gutowska, Grażyna Adler, Andrzej Ciechanowicz, Bogusław Machaliński, Dariusz Chlubek
Erschienen in:
European Journal of Nutrition
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Ausgabe 1/2007
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Abstract
The objective of this study was to assess the effect of conjugated linoleic acid isomers (CLAs) on the expression and activity of phospholipases A2 (PLA2) in human macrophages.
Macrophages were incubated with 30 µM cis-9, trans-11 and trans-10, cis-12 CLAs for 48 h. After incubation, the total activity of phospholipases as well as the expression of mRNA for cytosolic (cPLA2) and secretory (sPLA2) phospholipases and activity of sPLA2 were measured.
Both CLA isomers reduced the total activity of PLA2 (by 30.2%, P < 0.01 for cis-9, trans-11 CLA and by 30%, P < 0.001 for trans-10, cis-12 CLA). Trans-10, cis-12 CLA isomer downregulated the expression of mRNA of sPLA2 and decreased the enzymatic activity of this enzyme (by 23%, P = 0.02) in macrophages.
Conjugated linoleic acid isomers can significantly reduce the activity of PLA2 in macrophages and downregulate sPLA2 expression. The consequence of this effect may be reduction of releasing the arachidonic acid (AA) from the cellular membranes of macrophages.