nach oben

Inflammopharmacology

Erschienen in:

15.02.2017 | Original Article

Inhibition of proinflammatory pathways by bioactive fraction of Tinospora cordifolia

verfasst von: Jenny Jacob, Bashi M. Babu, Mohind C. Mohan, A. P. Abhimannue, B. Prakash Kumar

Erschienen in: Inflammopharmacology | Ausgabe 2/2018

Einloggen, um Zugang zu erhalten

Abstract

Tinospora cordifolia (Willd.) Miers ex Hook. f. & Thomson, a known immunomodulatory agent extensively used in ayurveda, has not been effectively validated for the mechanisms involved in immunomodulation and the identification of the active principles. The bioactive fraction of T. cordifolia (TBF) in methanol was used for nitric oxide (NO) radical scavenging activity, lipoxygenase (LOX) and cyclooxygenase (COX) dual inhibition and cytotoxicity studies. Production of the proinflammatory cytokines, tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in dendritic cell (DC) suspensions treated with lipopolysaccharide (LPS) was also studied. The bioactive principles involved were identified with ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometric (UPLC-Q-ToF MS/MS) system. The results indicate significantly higher potency of TBF as compared to positive standards for LOX/COX inhibition with moderate NO radical scavenging activity and the fraction was also found to be non-cytotoxic to monocyte cells. A significant inhibition was also observed in TNF-α and IL-1β production in LPS-treated DC suspensions as compared to standards, rolipram and dexamethasone, respectively. 11 compounds were identified from TBF by MS/MS system. The potent inhibition of LOX and COX enzymes with moderate NO scavenging was indicative of a free radical scavenging-independent mechanism of immunomodulation. Further investigations into the active principles identified would result in the development of lead candidates with potent therapeutic implications.

Nur mit Berechtigung zugänglich

Broderick KE, Feala J, McCulloch A, Paternostro G, Sharma VS, Pilz RB, Boss GR (2006) The nitric oxide scavenger cobinamide profoundly improves survival in a Drosophila melanogaster model of bacterial sepsis. FASEB J 20:1865–1873. doi:10.1096/fj.06-5780com CrossRefPubMed

Caminschi I, Lahoud MH, Shortman K (2009) Enhancing immune responses by targeting antigen to DC. Eur J Immunol 39:931–938. doi:10.1002/eji.200839035 CrossRefPubMed

Chang DM, Baptiste P, Schur PH (1990) The effect of antirheumatic drugs on interleukin 1 (IL-1) activity and IL-1 and IL-1 inhibitor production by human monocytes. J Rheumatol 17(9):1148–1157PubMed

Choi J-H, Do Y, Cheong C, Koh H, Boscardin SB, Oh Y-S, Bozzacco L, Trumpfheller C, Park CG, Steinman RM (2009) Identification of antigen-presenting dendritic cells in mouse aorta and cardiac valves. J Exp Med 206:497–505. doi:10.1084/jem.20082129 CrossRefPubMedPubMedCentral

Copeland RA, Williams JM, Giannaras J, Nurnberg S, Covington M, Pinto D, Pick S, Trzaskos JM (1994) Mechanism of selective inhibition of the inducible isoform of prostaglandin G/H synthase. Proc Natl Acad Sci USA 91:11202–11206CrossRefPubMedPubMedCentral

De Caterina R, Zampolli A (2004) From asthma to atherosclerosis—5-lipoxygenase, leukotrienes, and inflammation. N Engl J Med 350:4–7. doi:10.1056/NEJMp038190 CrossRefPubMed

Dinarello CA (2000) Proinflammatory cytokines. Chest 118:503–508CrossRefPubMed

Fiorucci S, Meli R, Bucci M, Cirino G (2001) Dual inhibitors of cyclooxygenase and 5-lipoxygenase. A new avenue in anti-inflammatory therapy? Biochem Pharmacol 62:1433–1438CrossRefPubMed

Gheorghe KR, Korotkova M, Catrina AI, Backman L, Af Klint E, Claesson H-E, Rådmark O, Jakobsson P-J (2009) Expression of 5-lipoxygenase and 15-lipoxygenase in rheumatoid arthritis synovium and effects of intraarticular glucocorticoids. Arthritis Res Ther 11:R83. doi:10.1186/ar2717 CrossRefPubMedPubMedCentral

Gordaliza M, García PA, del Corral JMM, Castro MA, Gómez-Zurita MA (2004) Podophyllotoxin: distribution, sources, applications and new cytotoxic derivatives. Toxicon 44:441–459. doi:10.1016/j.toxicon.2004.05.008 CrossRefPubMed

Hsi LC, Wilson LC, Eling TE (2002) Opposing effects of 15-Lipoxygenase-1 and -2 metabolites on MAPK signaling in prostate: alteration in peroxisome proliferator-activated receptor. J Biol Chem 277:40549–40556. doi:10.1074/jbc.M203522200 CrossRefPubMed

Jacob J, Kumar PB (2013) Ayurvedic herb, Tinospora cordifolia: validation of anti-inflammatory and immunomodulatory activity by effect on inflammatory mediators, TNF-α and lipoxygenase isozymes. JPRBioMedRx Int J 1(9):861–864

Jacob J, Prakash KB, Abhimannue AP, Mohan M, Babu BM (2014) Inhibition of lipoxygenase enzymes by extracts of Tinospora cordifolia: a study of enzyme kinetics. J Nat Prod 7:203–209

Kern JA, Lamb RJ, Reed JC, Daniele RP, Nowell PC (1988) Dexamethasone inhibition of interleukin 1 beta production by human monocytes. Posttranscriptional mechanisms. J Clin Invest 81(1):237–244CrossRefPubMedPubMedCentral

Korhonen R, Hömmö T, Keränen T, Laavola M, Hämäläinen M, Vuolteenaho K, Lehtimäki L, Kankaanranta H, Moilanen E (2013) Attenuation of TNF production and experimentally induced inflammation by PDE4 inhibitor rolipram is mediated by MAPK phosphatase-1. Br J Pharmacol 169(7):1525–1536CrossRefPubMedPubMedCentral

Lambrecht BN, Salomon B, Klatzmann D, Pauwels RA (1998) Dendritic cells are required for the development of chronic eosinophilic airway inflammation in response to inhaled antigen in sensitized mice. J Immunol 160:4090–4097PubMed

Li Z, Geng Y-N, Jiang J-D, Kong W-J (2014) Antioxidant and anti-inflammatory activities of berberine in the treatment of diabetes mellitus. Evid Based Complement Alternat Med 2014:289264. doi:10.1155/2014/289264 PubMedPubMedCentral

Marjanović M, Kralj M, Supek F, Frkanec L, Piantanida I, Smuc T, Tusek-Bozić L (2007) Antitumor potential of crown ethers: structure-activity relationships, cell cycle disturbances, and cell death studies of a series of ionophores. J Med Chem 50:1007–1018. doi:10.1021/jm061162u CrossRefPubMed

Palmer G, Guerne PA, Mezin F, Maret M, Guicheux J, Goldring MB, Gabay C (2002) Production of interleukin-1 receptor antagonist by human articular chondrocytes. Arthritis Res Therapy 4(3):226–231CrossRef

Parente L (2001) Pros and cons of selective inhibition of cyclooxygenase-2 versus dual lipoxygenase/cyclooxygenase inhibition: is two better than one? J Rheumatol 28:2375–2382PubMed

Pereira BJG, Shapiro L, King AJ, Falagas ME, Strom JA, Dinarello CA (1994) Plasma levels of IL-1β, TNFα and their specific inhibitors in undialyzed chronic renal failure, CAPD and hemodialysis patients. Kidney Int 45:890–896. doi:10.1038/ki.1994.117 CrossRefPubMed

Pinto NB, Morais TC, Carvalho KMB, Silva CR, Andrade GM, Brito GAC, Veras ML, Pessoa ODL, Rao VS, Santos FA (2010) Topical anti-inflammatory potential of Physalin E from Physalis angulata on experimental dermatitis in mice. Phytomedicine 17(10):740–743. doi:10.1016/j.phymed.2010.01.006 CrossRefPubMed

Singh BP, Deepa (2015) Concept of immunomodulation in ayurveda and some immunomodulatory herbs. Int Ayurvedic Med J 3(7):2205–2212

Souness JE, Griffin M, Maslen C, Ebsworth K, Scott LC, Pollock K, Palfreyman MN, Karlsson JA (1996) Evidence that cyclic AMP phosphodiesterase inhibitors suppress TNFα generation from human monocytes by interacting with a ‘low-affinity’ phosphodiesterase 4 conformer. Br J Pharmacol 118(3):649–658CrossRefPubMedPubMedCentral

Steinman RM (1991) The dendritic cell system and its role in immunogenicity. Annu Rev Immunol 9:271–296. doi:10.1146/annurev.iy.09.040191.001415 CrossRefPubMed

Tsai P-J, Tsai T-H, Yu C-H, Ho S-C (2007) Comparison of NO-scavenging and NO-suppressing activities of different herbal teas with those of green tea. Food Chem 103:181–187. doi:10.1016/j.foodchem.2006.08.013 CrossRef

Verma R, Juvekar AR, Gopalkrishnan R (2006) Bioassay guided purification of an immunomodulatory polysaccharide from roots of Tinospora cordifolia. Planta Medica 72(11):P_002. doi:10.1055/s-2006-949802

Wang P, Myers JG, Wu P, Cheewatrakoolpong B, Egan RW, Billah MM (1997) Expression, purification, and characterization of human cAMP-specific phosphodiesterase (PDE4) subtypes A, B, C, and D. Biochem Biophys Res Commun 234:320–324CrossRefPubMed

Yang K, Ma W, Liang H, Ouyang Q, Tang C, Lai L (2007) Dynamic simulations on the arachidonic acid metabolic network. PLoS Comput Biol 3:e55. doi:10.1371/journal.pcbi.0030055 CrossRefPubMedPubMedCentral

Zhang J-M, An J (2007) Cytokines, inflammation, and pain. Int Anesthesiol Clin 45:27–37. doi:10.1097/AIA.0b013e318034194e CrossRefPubMedPubMedCentral

Titel: Inhibition of proinflammatory pathways by bioactive fraction of Tinospora cordifolia
verfasst von: Jenny Jacob
Bashi M. Babu
Mohind C. Mohan
A. P. Abhimannue
B. Prakash Kumar
Publikationsdatum: 15.02.2017
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 2/2018
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0319-2

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2018

Evaluation of cebranopadol, a dually acting nociceptin/orphanin FQ and opioid receptor agonist in mouse models of acute, tonic, and chemotherapy-induced neuropathic pain

Curcumin affords neuroprotection and inhibits α-synuclein aggregation in lipopolysaccharide-induced Parkinson’s disease model

Diethylcarbamazine attenuates the expression of pro-fibrogenic markers and hepatic stellate cells activation in carbon tetrachloride-induced liver fibrosis

Anti-inflammatory evaluation and characterization of leaf extract of Ananas comosus

Acid-gastric antisecretory effect of the ethanolic extract from Arctium lappa L. root: role of H+, K+-ATPase, Ca2+ influx and the cholinergic pathway

Arctium minus crude extract presents antinociceptive effect in a mice acute gout attack model