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Inflammopharmacology

Erschienen in:

01.04.2017 | Original Article

Inhibitory effects of Dioscin on atherosclerosis and foam cell formation in hyperlipidemia rats

verfasst von: Ping Wang, Li-ya He, Guo-dong Shen, Rui-lin Li, Jun-li Yang

Erschienen in: Inflammopharmacology | Ausgabe 6/2017

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Abstract

Macrophage-derived foam cells are well known for their key role in development of atherosclerosis (AS). The present study aimed to examine whether dioscin exerts anti-atherosclerotic activity and inhibits foam cell formation. A high-fat induced AS model and ox-LDL treated macrophages were established and received treatment of dioscin. Anti-atherosclerotic activity in vivo was assessed by atherosclerotic lesions size and aortic lipid contents. Macrophage formed foam cells were positively identified by oil red o staining. Moreover, the expression of LOX-1 and NF-κB in aorta tissue and macrophages was examined by western blotting assay. Our results showed that dioscin not only reduced the levels of plasma lipid, TNF-a, IL-1β and IL-6, but also inhibited atherosclerotic development in AS rats, as evidenced by decreased atherosclerotic lesions size and aortic lipid level. In vitro study revealed dioscin directly reduced foam cell formation, decreased intracellular cholesterol accumulation and lowered TNF-a, IL-1β and IL-6 secretion in ox-LDL treated macrophages. Interestingly, further work found dioscin significantly reduced expression of LOX-1 and NF-κB in the aortic tissue and ox-LDL treated macrophages. In summary, our study was the first to confirm anti-atherosclerotic activity of dioscin in vivo and vitro. Moreover, the other important finding is dioscin mediated ox-LDL/LOX-1/NF-κB regulated contributions to the attenuate macrophage ox-LDL uptake and AS.

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Titel: Inhibitory effects of Dioscin on atherosclerosis and foam cell formation in hyperlipidemia rats
verfasst von: Ping Wang
Li-ya He
Guo-dong Shen
Rui-lin Li
Jun-li Yang
Publikationsdatum: 01.04.2017
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 6/2017
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0341-4

Springer Medizin

Abstract

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