Background
Methods
Study population
Information collection
DNA preparation
SNP genotyping
Statistical analysis
Results
Demographic, behavioral information and clinical characteristics of participants
Variable | Variable frequencies (%) | |||
---|---|---|---|---|
Controls (N = 308) | MI patients (N = 502) | t/χ2
|
p-value | |
Age
| 61.87 ± 10.39 | 63.66 ± 11.57 | 2.2823 | 0.0228 |
BMI (kg/m
2
)
| 25.84 ± 3.53 | 25.73 ± 3.09 | 0.4673 | 0.6404 |
Gender
| ||||
Male | 47.73 | 77.09 | 73.2707 | <.0001 |
Female | 52.27 | 22.91 | ||
Ethnicity
| ||||
Chinese Han | 94.48 | 96.81 | 2.6567 | 0.1031 |
Ethnicity minority | 5.52 | 3.19 | ||
Smoking habit
| ||||
No | 63.96 | 36.65 | 57.1381 | <.0001 |
Yes | 36.04 | 63.35 | ||
Drinking habit
| ||||
No | 74.68 | 61.75 | 14.3438 | 0.0002 |
Yes | 25.32 | 38.25 | ||
T2DM
| ||||
No | 75.97 | 61.35 | 18.4275 | <.0001 |
Yes | 24.03 | 38.65 | ||
Hypertension
| ||||
No | 35.71 | 32.07 | 1.1376 | 0.2862 |
Yes | 64.29 | 67.93 | ||
Hyperlipidemia
| ||||
No | 60.06 | 61.55 | 0.1778 | 0.6732 |
Yes | 39.94 | 38.45 |
Association analysis of genetic polymorphisms with MI
Chromosome location | Closest gene | SNPs | Genotypes | Genotype frequencies (%) | Without adjustment | With adjustment | |||
---|---|---|---|---|---|---|---|---|---|
Controls (N = 308) | MI patients (N = 502) | OR (95% CI) |
p
| ORa(95% CI) |
p
a
| ||||
6P24 | ADTRP | rs6903956 | GG | 85.23 | 79.30 | reference | |||
GA | 13.40 | 18.74 | 1.50 (1.00,2.27) | 0.0525 | 1.55 (0.99,2.43) | 0.0556 | |||
AA | 1.37 | 1.96 | 1.53 (0.47,5.03) | 0.4813 | 1.04 (0.30,3.59) | 0.9522 | |||
AA/GA | 14.78 | 20.70 | 1.51 (1.01,2.23) | 0.0423 | 1.49(0.97,2.29) | 0.0678 | |||
9P21 | CDKN2A/B | rs10757274 | AA | 32.78 | 23.54 | reference | |||
GA | 51.32 | 52.52 | 1.43 (1.02,1.99) | 0.0374 | 1.52(1.06,2.19) | 0.0227 | |||
GG | 15.89 | 23.94 | 2.10 (1.37,3.22) | 0.0007 | 2.40 (1.51,3.81) | 0.0002 | |||
GG/GA | 67.22 | 76.46 | 1.58 (1.15,2.18) | 0.0045 | 1.72 (1.22,2.43) | 0.0020 |
Gene-environment interactions analysis
Modelsa
| Prediction accuracy | CVC |
p
sign
|
p
perm
|
---|---|---|---|---|
T2DM | 0.5815 | 10/10 | 0.0010 | <0.001 |
T2DM, rs10757274 | 0.5918 | 10/10 | 0.0107 | <0.001 |
T2DM, rs10757274, rs6903956 | 0.5796 | 10/10 | 0.0107 | <0.001 |
T2DM, hyperlipidemia, rs10757274, rs6903956 | 0.5815 | 9/10 | 0.0547 | <0.001 |
T2DM, hyperlipidemia, hypertension, rs10757274, rs6903956 | 0.5692 | 10/10 | 0.0547 | 0.008 |
Frequencies (%) | |||||||
---|---|---|---|---|---|---|---|
Genotype | T2DM | Controls (N = 308) | MI patients (N = 502) | ORadd
a(95% CI) |
p
add
| ORmulti
a(95% CI) |
p
multi
|
rs10757274 | 1.63(0.78,3.44) | 0.197 | |||||
AA | No | 22.52 | 12.67 | reference | |||
GG/GA | 53.31 | 48.69 | 1.60 (1.04,2.46) | 0.0329 | |||
AA | Yes | 10.26 | 10.87 | 1.68 (0.92,3.08) | 0.0943 | ||
GG/GA | 13.91 | 27.77 | 4.38 (2.56,7.47) | <0.0001 | |||
RERI | 2.10 (0.18,4.03) | ||||||
API | 0.48 (0.19,0.77) | ||||||
S | 2.65(1.01,6.94) |