Electronic supplementary material
Materials and methods
Preparation of tissue homogenates
Myeloperoxidase (MPO) assay
Quantitative real-time reverse transcriptase (RT)-PCR analysis
Stability of the model
IFN-β inhibits the influx of neutrophils into the lung
IFN-β inhibits the expression of endothelial activation markers and pulmonary chemokines
IFN-β reduces pulmonary TNF-α expression
Subarachnoid hemorrhage in rats is associated with increased pulmonary inflammatory mediators.
Subarachnoid hemorrhage results in increased neutrophil influx into the lung.
IFN-β treatment completely abolishes the subarachnoid hemorrhage-induced pulmonary inflammation.