Interleukin-32γ Enhances the Production of IL-6 and IL-8 in Fibroblast-Like Synoviocytes Via Erk1/2 Activation
Yong-Gil Kim, Chang-Keun Lee, Soo-Hyun Kim, Woo-Shin Cho, Se Hwan Mun, Bin Yoo
Journal of Clinical Immunology
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In the present study, we examined the effect of the pro-inflammatory cytokine IL-32γ, the most biologically active isoform, and its related molecules in fibroblast-like synoviocytes (FLS).
Materials and Methods
FLS were isolated from synovial tissues of rheumatoid arthritis (RA) patients. The secretion and expression of IL-6 and IL-8 were examined by ELISA and real-time PCR, and the activation of signaling molecules was evaluated by Western blot, electrophoretic mobility shift assay (EMSA), real-time PCR, and siRNA transfection.
By IL-32γ stimulation in RA FLS, the expressions of IL-6 and IL-8 were increased significantly, and the phosphorylated Erk1/2 and AP-1 were expressed prominently in Western blot and EMSA. In the Erk1/2 inhibited cells, IL-32γ stimulation did not increase the mRNA expression of IL-6 and IL-8.
Our results suggest that IL-32γ stimulation can induce the production of IL-6 and IL-8 from RA FLS via Erk1/2 activation.