Skip to main content
main-content

01.12.2012 | Research | Ausgabe 1/2012 Open Access

Journal of Inflammation 1/2012

Intra-coronary administration of tacrolimus markedly attenuates infarct size and preserves heart function in porcine myocardial infarction

Zeitschrift:
Journal of Inflammation > Ausgabe 1/2012
Autoren:
Sarah Chua, Steve Leu, Jiunn-Jye Sheu, Yu-Chun Lin, Li-Teh Chang, Ying-Hsien Kao, Chia-Hung Yen, Tzu-Hsien Tsai, Yung-Lung Chen, Hsueh-Wen Chang, Cheuk-Kwan Sun, Hon-Kan Yip
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1476-9255-9-21) contains supplementary material, which is available to authorized users.
Sarah Chua, Steve Leu contributed equally to this work.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

All authors have read and approved the final manuscript.
SC, SL, CKS, JJS, and HKY designed the experiment, drafted and performed animal experiments. LTC, THT, YLC, YHK, and CHY were responsible for the laboratory assay and troubleshooting. HWC, SL and HKY participated in refinement of experiment protocol and coordination and helped in drafting the manuscript.

Abstract

Background

We test the hypothesis that intra-coronary tacrolimus administration can limit infarct size and preserve left ventricular ejection fraction (LVEF) after acute myocardial infarction (AMI) through ligating left anterior descending coronary artery (LAD) in mini-pigs.

Methods

Twelve male mini-pigs were randomized into AMI-saline (MI-only) group and AMI-tacrolimus (MI-Tac) group that received intra-coronary saline (3.0 mL) and tacrolimus (0.5 mg in 2.5 mL saline) injection, respectively, beyond site of ligation 30 minutes after LAD occlusion.

Results

Larger infarct area was noted in MI-only group (p < 0.001). Inflammatory biomarkers at protein [oxidative stress, tumor necrotic factor-α, nuclear factor-κB], gene (matrix metalloproteinase-9, plasminogen activator inhibitor-1), and cellular (CD40+, CD68+ inflammatory cells) levels were remarkably higher in MI-only animals (p < 0.01). Conversely, anti-inflammatory biomarkers at gene level (Interleukin-10), gene and protein level (endothelial nitric oxide synthase), and anti-oxidant biomarkers at both gene and protein levels [heme oxygenase 1, NAD(P)H:quinone oxidoreductase] were lower in MI-only group (p < 0.01). Number of apoptotic nuclei and apoptotic biomarkers expressions at gene and protein levels (Bax, caspase 3) were notably higher, whereas anti-apoptotic biomarkers at gene and protein levels (Bcl-2), LVEF, and fractional shortening were markedly lower in MI-only group (p < 0.001).

Conclusion

Intra-coronary administration of tacrolimus significantly attenuated infarct size and preserved LV function.
Zusatzmaterial
Authors’ original file for figure 1
12950_2011_236_MOESM1_ESM.jpeg
Authors’ original file for figure 2
12950_2011_236_MOESM2_ESM.jpeg
Authors’ original file for figure 3
12950_2011_236_MOESM3_ESM.jpeg
Authors’ original file for figure 4
12950_2011_236_MOESM4_ESM.jpeg
Authors’ original file for figure 5
12950_2011_236_MOESM5_ESM.jpeg
Authors’ original file for figure 6
12950_2011_236_MOESM6_ESM.jpeg
Authors’ original file for figure 7
12950_2011_236_MOESM7_ESM.jpeg
Authors’ original file for figure 8
12950_2011_236_MOESM8_ESM.jpeg
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2012

Journal of Inflammation 1/2012 Zur Ausgabe

Neu im Fachgebiet Innere Medizin

Meistgelesene Bücher aus der Inneren Medizin

2017 | Buch

Rheumatologie aus der Praxis

Entzündliche Gelenkerkrankungen – mit Fallbeispielen

Dieses Fachbuch macht mit den wichtigsten chronisch entzündlichen Gelenk- und Wirbelsäulenerkrankungen vertraut. Anhand von über 40 instruktiven Fallbeispielen werden anschaulich diagnostisches Vorgehen, therapeutisches Ansprechen und der Verlauf …

Herausgeber:
Rudolf Puchner

2016 | Buch

Ambulant erworbene Pneumonie

Was, wann, warum – Dieses Buch bietet differenzierte Diagnostik und Therapie der ambulant erworbenen Pneumonie zur sofortigen sicheren Anwendung. Entsprechend der neuesten Studien und Leitlinien aller wichtigen Fachgesellschaften.

Herausgeber:
Santiago Ewig

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Innere Medizin und bleiben Sie gut informiert – ganz bequem per eMail.

© Springer Medizin 

Bildnachweise