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Erschienen in: Lasers in Medical Science 1/2021

27.06.2020 | Original Article

Intra-volume processing of gelatine hydrogel by femtosecond laser-induced cavitation

verfasst von: Isabel Vérit, Laura Gemini, Jean-Christophe Fricain, Rainer Kling, Claire Rigothier

Erschienen in: Lasers in Medical Science | Ausgabe 1/2021

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Abstract

Cell oxygenation and nutrition are crucial for the viability of tissue-engineered constructs, and different alternatives are currently being developed to achieve an adequate vascularisation of the engineered tissue. One of the alternatives is the generation of channel-like patterns in a bioconstruct. Here, the formation of full-formed channels inside hydrogels by laser-induced cavitation was investigated. A near-infrared, femtosecond laser beam focused with a high numerical aperture was employed to obtain intra-volume modifications of a block of gelatine hydrogel. Characterisation of the laser-processed gelatine was carried out by optical microscopy and epifluorescence microscopy right after and 24 h after the laser process. Rheology analyses on the unprocessed gelatine blocks were conducted to better understand the cavitation mechanism taking place during the intense laser interaction. Different cavitation patterns were observed at varying dose values by changing the repetition rate and the overlap between successive pulses while keeping the laser fluence and the number of passes fixed. This way, cavitation bubble features and behaviour can be controlled to optimise the formation of intra-volume channels in the gelatine volume. Results showed that the generation of fully formed channels was linked to the formation of large non-spherical cavitation bubbles during the laser interaction at high dose and low repetition rates. In conclusion, the formation of fully formed channels was made possible with a near-infrared, femtosecond laser beam strongly focused inside gelatine hydrogel blocks through laser-induced cavitation at high dose and low repetition rates.
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Metadaten
Titel
Intra-volume processing of gelatine hydrogel by femtosecond laser-induced cavitation
verfasst von
Isabel Vérit
Laura Gemini
Jean-Christophe Fricain
Rainer Kling
Claire Rigothier
Publikationsdatum
27.06.2020
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 1/2021
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-020-03081-4

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