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Pediatric Surgery International
Erschienen in:

22.08.2022 | Original Article

Intrauterine exposure to oxidative stress induces caspase-1-dependent enteric nerve cell pyroptosis

verfasst von: Lingling Zhou, Bingyu Wang, Hua Xie, Chunxia Du, Jie Tang, Weibing Tang

Erschienen in: Pediatric Surgery International | Ausgabe 11/2022

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Abstract

Purpose

This study determined whether oxidative stress causes the developmental abnormalities of the enteric nervous system during the embryonic period.

Methods

Using the test results of tissue specimens of children with Hirschsprung disease (HSCR), we established a pregnant rat model of oxidative stress and a cellular oxidative stress model to conduct related molecular, cellular, and histopathological experiments for exploration and validation.

Results

The results of the quantitative real-time polymerase chain reaction assay indicated overexpression of pyroptosis markers (NLRP3, ASC, and caspase-1) in HSCR lesions and newborn pups in the oxidative stress group (treated with d-galactose). The expression of cathepsin D was significantly decreased in intestinal tissues of newborn pups in the oxidative stress group compared to the control group. Reactive oxygen species scavengers (N-acetyl-cysteine, NAC), the caspase-1 inhibitor (VX-765), and the NLRP3 siRNA could reverse the release of LDH, decrease the number of propidium iodide stained cells, and reduce the percentage of TUNEL/caspase-3 double-positive cells in the H2O2-treated group.

Conclusion

Oxidative stress can induce the death of enteric nerve cells by activating caspase-1-dependent pyroptosis through NLRP3 inflammasomes, which may contribute to abnormal enteric nervous system development.
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Literatur
1.
Furness JB, Callaghan BP, Rivera LR, Cho HJ (2014) The enteric nervous system and gastrointestinal innervation: integrated local and central control. Adv Exp Med Biol 817:39–71PubMedCrossRef
2.
3.
Kapur RP (2000) Colonization of the murine hindgut by sacral crest-derived neural precursors: experimental support for an evolutionarily conserved model. Dev Biol 227:146–155PubMedCrossRef
4.
Heuckeroth RO (2018) Hirschsprung disease—integrating basic science and clinical medicine to improve outcomes. Nat Rev Gastroenterol Hepatol 15:152–167PubMedCrossRef
5.
Goldstein AM, Cox NJ (2019) Complex simplicity and Hirschsprung’s disease. N Engl J Med 380:1478–1479PubMedCrossRef
6.
Chatterjee S, Nandakumar P, Auer DR, Gabriel SB, Chakravarti A (2019) Gene- and tissue-level interactions in normal gastrointestinal development and Hirschsprung disease. Proc Natl Acad Sci USA 116:26697–26708PubMedCentralCrossRef
7.
Jaroy EG, Acosta-Jimenez L, Hotta R, Goldstein AM, Emblem R, Klungland A, Ougland R (2019) “Too much guts and not enough brains”: (epi)genetic mechanisms and future therapies of Hirschsprung disease—a review. Clin Epigenetics 11:135PubMedPubMedCentralCrossRef
8.
Lake JI, Heuckeroth RO (2013) Enteric nervous system development: migration, differentiation, and disease. Am J Physiol Gastrointest Liver Physiol 305:G1-24PubMedPubMedCentralCrossRef
9.
Butler Tjaden NE, Trainor PA (2013) The developmental etiology and pathogenesis of Hirschsprung disease. Transl Res 162:1–15PubMedCrossRef
10.
Akbareian SE, Nagy N, Steiger CE, Mably JD, Miller SA, Hotta R, Molnar D, Goldstein AM (2013) Enteric neural crest-derived cells promote their migration by modifying their microenvironment through tenascin-C production. Dev Biol 382:446–456PubMedPubMedCentralCrossRef
11.
Nakazawa N, Miyahara K, Okawada M, Yamataka A, Suzuki R, Akazawa C, Tomikawa-Ichikawa N, Arikawa-Hirasawa E (2013) Laminin-1 promotes enteric nervous system development in mouse embryo. Pediatr Surg Int 29:1205–1208PubMedCrossRef
12.
13.
Nagy N, Guyer RA, Hotta R, Zhang D, Newgreen DF, Halasy V, Kovacs T, Goldstein AM (2020) RET overactivation leads to concurrent Hirschsprung disease and intestinal ganglioneuromas. Development 147:dev190900PubMedPubMedCentralCrossRef
14.
Wen Z, Shen Q, Zhang H, Su Y, Zhu Z, Chen G, Peng L, Li H, Du C, Xie H, Xu X, Tang W (2019) Circular RNA CCDC66 targets DCX to regulate cell proliferation and migration by sponging miR-488-3p in Hirschsprung’s disease. J Cell Physiol 234:10576–10587PubMedCrossRef
15.
Su Y, Wen Z, Shen Q, Zhang H, Peng L, Chen G, Zhu Z, Du C, Xie H, Li H, Xia Y, Tang W (2018) Long non-coding RNA LOC100507600 functions as a competitive endogenous RNA to regulate BMI1 expression by sponging miR128–1–3p in Hirschsprung’s disease. Cell Cycle 17:1–9CrossRef
16.
Hameister R, Kaur C, Dheen ST, Lohmann CH, Singh G (2020) Reactive oxygen/nitrogen species (ROS/RNS) and oxidative stress in arthroplasty. J Biomed Mater Res B Appl Biomater 108:2073–2087PubMedCrossRef
17.
Fussell JC, Kelly FJ (2019) Oxidative contribution of air pollution to extrinsic skin ageing. Free Radic Biol Med 151:111–122PubMedCrossRef
18.
Kato M, Iwashita T, Takeda K, Akhand AA, Liu W, Yoshihara M, Asai N, Suzuki H, Takahashi M, Nakashima I (2000) Ultraviolet light induces redox reaction-mediated dimerization and superactivation of oncogenic ret tyrosine kinases. Mol Biol Cell 11:93–101PubMedPubMedCentralCrossRef
19.
20.
21.
Mullen L, Mengozzi M, Hanschmann EM, Alberts B, Ghezzi P (2019) How the redox state regulates immunity. Free Radic Biol Med 157:3–14PubMedCrossRef
22.
Kramer ER, Aron L, Ramakers GM, Seitz S, Zhuang X, Beyer K, Smidt MP, Klein R (2007) Absence of Ret signaling in mice causes progressive and late degeneration of the nigrostriatal system. PLoS Biol 5:e39PubMedPubMedCentralCrossRef
23.
Kato M, Ninomiya H, Maeda M, Tanaka N, Ilmiawati C, Yoshinaga M (2016) Commentary to Gorelenkova Miller and Mieyal (2015): sulfhydryl-mediated redox signaling in inflammation: role in neurodegenerative diseases. Arch Toxicol 90:1017–1018PubMedCrossRef
24.
Hossain K, Akhand AA, Kato M, Du J, Takeda K, Wu J, Takeuchi K, Liu W, Suzuki H, Nakashima I (2000) Arsenite induces apoptosis of murine T lymphocytes through membrane raft-linked signaling for activation of c-Jun amino-terminal kinase. J Immunol 165:4290–4297PubMedCrossRef
25.
Guo YL, Chakraborty S, Rajan SS, Wang R, Huang F (2010) Effects of oxidative stress on mouse embryonic stem cell proliferation, apoptosis, senescence, and self-renewal. Stem Cells Dev 19:1321–1331PubMedPubMedCentralCrossRef
26.
Cho KA, Suh JW, Lee KH, Kang JL, Woo SY (2012) IL-17 and IL-22 enhance skin inflammation by stimulating the secretion of IL-1beta by keratinocytes via the ROS-NLRP3-caspase-1 pathway. Int Immunol 24:147–158PubMedCrossRef
27.
Xue Y, Enosi Tuipulotu D, Tan WH, Kay C, Man SM (2019) Emerging activators and regulators of inflammasomes and pyroptosis. Trends Immunol 40:1035–1052PubMedCrossRef
28.
Shi J, Gao W, Shao F (2017) Pyroptosis: gasdermin-mediated programmed necrotic cell death. Trends Biochem Sci 42:245–254PubMedCrossRef
29.
Winkler S, Rosen-Wolff A (2015) Caspase-1: an integral regulator of innate immunity. Seminars in immunopathology 37:419–427PubMedCrossRef
30.
31.
Gulbransen BD, Bashashati M, Hirota SA, Gui X, Roberts JA, MacDonald JA, Muruve DA, McKay DM, Beck PL, Mawe GM, Thompson RJ, Sharkey KA (2012) Activation of neuronal P2X7 receptor-pannexin-1 mediates death of enteric neurons during colitis. Nat Med 18:600–604PubMedPubMedCentralCrossRef
32.
Fann DY, Lee SY, Manzanero S, Chunduri P, Sobey CG, Arumugam TV (2013) Pathogenesis of acute stroke and the role of inflammasomes. Ageing Res Rev 12:941–966PubMedCrossRef
33.
Ishrat T, Mohamed IN, Pillai B, Soliman S, Fouda AY, Ergul A, El-Remessy AB, Fagan SC (2015) Thioredoxin-interacting protein: a novel target for neuroprotection in experimental thromboembolic stroke in mice. Mol Neurobiol 51:766–778PubMedCrossRef
34.
Crowley LC, Marfell BJ, Waterhouse NJ (2016) Detection of DNA fragmentation in apoptotic cells by TUNEL. Cold Spring Harb Protoc 2016:pdb-prot087221CrossRef
35.
Abu-Alfa AK, Kuan SF, West AB, Reyes-Múgica M (1997) Cathepsin D in intestinal ganglion cells. A potential aid to diagnosis in suspected Hirschsprung’s disease. Am J Surg Pathol 21:201–205PubMedCrossRef
36.
Karaca G, Karaca ZM, Kayhan B, Bayindir Y, Kayabas U, Toplu S, Elmasdag S, Onalan E, Yesilada E (2019) The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. North Clin Istanb 6:117–123PubMed
37.
38.
Wang Y, Jiang Q, Cai H, Xu Z, Wu W, Gu B, Li L, Cai W (2020) Genetic variants in RET, ARHGEF3 and CTNNAL1, and relevant interaction networks, contribute to the risk of Hirschsprung disease. Aging (Albany NY). 12:4379PubMedPubMedCentralCrossRef
39.
Heanue TA, Pachnis V (2007) Enteric nervous system development and Hirschsprung’s disease: advances in genetic and stem cell studies. Nat Rev Neurosci 8:466–479PubMedCrossRef
40.
Lotfollahzadeh S, Taherian M, Anand S (2020) Hirschsprung disease. StatPearls, Treasure Island
41.
Jiang Q, Arnold S, Heanue T, Kilambi KP, Doan B, Kapoor A, Ling AY, Sosa MX, Guy M, Jiang Q, Burzynski G, West K, Bessling S, Griseri P, Amiel J, Fernandez RM, Verheij JB, Hofstra RM, Borrego S, Lyonnet S, Ceccherini I, Gray JJ, Pachnis V, McCallion AS, Chakravarti A (2015) Functional loss of semaphorin 3C and/or semaphorin 3D and their epistatic interaction with ret are critical to Hirschsprung disease liability. Am J Hum Genet 96:581–596PubMedPubMedCentralCrossRef
42.
Ye L, Li G, Goebel A, Raju AV, Kong F, Lv Y, Li K, Zhu Y, Raja S, He P, Li F, Mwangi SM, Hu W, Srinivasan S (2020) Caspase-11-mediated enteric neuronal pyroptosis underlies Western diet-induced colonic dysmotility. J Clin Investig 130:3621–3636PubMedPubMedCentralCrossRef
43.
44.
Kuwar R, Rolfe A, Di L, Xu H, He L, Jiang Y, Zhang S, Sun D (2019) A novel small molecular NLRP3 inflammasome inhibitor alleviates neuroinflammatory response following traumatic brain injury. J Neuroinflammation 16:81PubMedPubMedCentralCrossRef
45.
Li H, Zhou L, Zhi Z, Lv X, Wei Z, Zhang X, Tang W, Tong M (2020) Lipopolysaccharide upregulates miR-132/212 in Hirschsprung-associated enterocolitis, facilitating pyroptosis by activating NLRP3 inflammasome via targeting Sirtuin 1 (SIRT1). Aging 12:18588–18602PubMedPubMedCentralCrossRef
46.
Chen X, Liu G, Yuan Y, Wu G, Wang S, Yuan L (2019) NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-kappaB signaling. Cell Death Dis 10:906PubMedPubMedCentralCrossRef
47.
Pastor AC, Osman F, Teitelbaum DH, Caty MG, Langer JC (2009) Development of a standardized definition for Hirschsprung’s-associated enterocolitis: a delphi analysis. J Pediatr Surg 44:251–256PubMedCrossRef
48.
49.
Ali SS, Ahsan H, Zia MK, Siddiqui T, Khan FH (2020) Understanding oxidants and antioxidants: classical team with new players. J Food Biochem 44:e13145PubMedCrossRef
50.
Yagami T, Yamamoto Y, Koma H (2019) Pathophysiological roles of intracellular proteases in neuronal development and neurological diseases. Mol Neurobiol 56:3090–3112PubMedCrossRef
51.
Bhatia S, Drake DM, Miller L, Wells PG (2019) Oxidative stress and DNA damage in the mechanism of fetal alcohol spectrum disorders. Birth Defects Res 111:714–748PubMedCrossRef
52.
Aminzadeh M, Roghani M, Sarfallah A, Riazi GH (2018) TRPM2 dependence of ROS-induced NLRP3 activation in Alzheimer’s disease. Int Immunopharmacol 54:78–85PubMedCrossRef
53.
Ganjam GK, Bolte K, Matschke LA, Neitemeier S, Dolga AM, Hollerhage M, Hoglinger GU, Adamczyk A, Decher N, Oertel WH, Culmsee C (2019) Mitochondrial damage by alpha-synuclein causes cell death in human dopaminergic neurons. Cell Death Dis 10:865PubMedPubMedCentralCrossRef
54.
55.
Penn RB (2021) Honing in on the effectors of oxidative stress in the asthmatic lung: oxidised phosphatidylcholines. Eur Respir J 57:2003736PubMedCrossRef
56.
Nakao A, Matsunaga Y, Hayashida K, Takahashi N (2021) Role of oxidative stress and Ca(2+) signaling in psychiatric disorders. Front Cell Dev Biol 9:615569PubMedPubMedCentralCrossRef
57.
Liu N, Lin MM, Huang SS, Liu ZQ, Wu JC, Liang ZQ, Qin ZH, Wang Y (2021) NADPH and mito-apocynin treatment protects against KA-induced excitotoxic injury through autophagy pathway. Front Cell Dev Biol 9:612554PubMedPubMedCentralCrossRef
58.
Ricke KM, Pass T, Kimoloi S, Fahrmann K, Jungst C, Schauss A, Baris OR, Aradjanski M, Trifunovic A, Eriksson Faelker TM, Bergami M, Wiesner RJ (2020) Mitochondrial dysfunction combined with high calcium load leads to impaired antioxidant defense underlying the selective loss of nigral dopaminergic neurons. J Neurosci 40:1975–1986PubMedPubMedCentralCrossRef
59.
Parajuli B, Sonobe Y, Horiuchi H, Takeuchi H, Mizuno T, Suzumura A (2013) Oligomeric amyloid beta induces IL-1beta processing via production of ROS: implication in Alzheimer’s disease. Cell Death Dis 4:e975PubMedPubMedCentralCrossRef
60.
Elsas LJ (2001) Prenatal diagnosis of galactose-l-phosphate uridyltransferase (GALT)-deficient galactosemia. Prenat Diagn 21:302–303PubMedCrossRef
61.
Gubbels CS, Land JA, Rubio-Gozalbo ME (2008) Fertility and impact of pregnancies on the mother and child in classic galactosemia. Obstet Gynecol Surv 63:334–343PubMedCrossRef
62.
Yu F, Hao S, Zhao Y, Yang H, Fan XL, Yang J (2011) In utero and lactational beta-carotene supplementation attenuates d-galactose-induced hearing loss in newborn rats. Food Chem Toxicol 49:1697–1704PubMedCrossRef
Metadaten
Titel
Intrauterine exposure to oxidative stress induces caspase-1-dependent enteric nerve cell pyroptosis
verfasst von
Lingling Zhou
Bingyu Wang
Hua Xie
Chunxia Du
Jie Tang
Weibing Tang
Publikationsdatum
22.08.2022
Verlag
Springer Berlin Heidelberg
Erschienen in
Pediatric Surgery International / Ausgabe 11/2022
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI
https://doi.org/10.1007/s00383-022-05199-8

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