Skip to main content
Erschienen in: Cancer Chemotherapy and Pharmacology 6/2019

10.04.2019 | Original Article

Investigation of anti-tumor effect of doxorubicin-loaded human serum albumin nanoparticles prepared by a desolvation technique

verfasst von: Kotaro Kimura, Keishi Yamasaki, Koji Nishi, Kazuaki Taguchi, Masaki Otagiri

Erschienen in: Cancer Chemotherapy and Pharmacology | Ausgabe 6/2019

Einloggen, um Zugang zu erhalten

Abstract

Purpose

Nanoparticles of human serum albumin (HSA) prepared using a desolvation method possess sizes suitable for tumor accumulation. Here, we report on an investigation of the anti-tumor effects and biodistribution of doxorubicin–HSA nanoparticles in vitro and in vivo.

Methods

The cytotoxicity of nanoparticles was evaluated in 2D and 3D colon 26 cell cultures. Furthermore, the biodistribution and the anti-tumor activity of nanoparticles in colon 26-bearing mice were investigated. Assessments on the effect on metastasis and the toxicity were also carried out.

Results

Doxorubicin–HSA nanoparticles showed cytotoxicity in colon 26 cancer cell cultures, although the cytotoxicity was less in the case of nanoparticles than in free doxorubicin. In vivo anti-tumor activity was more pronounced in nanoparticles despite the fact that their accumulation in tumors was not superior to that of free doxorubicin, suggesting that factors other than accumulation contribute to the enhanced anti-tumor activity of these nanoparticles. The administration of nanoparticles also resulted in the suppression of metastasis.

Conclusions

The prepared nanoparticles appear to be effective for cancer therapy although further studies will be needed to clarify the details of anti-tumor activity and the toxicity of these nanoparticles.
Literatur
1.
Zurück zum Zitat Hobbs SK, Monsky WL, Yuan F et al (1998) Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci USA 95:4607–4612CrossRef Hobbs SK, Monsky WL, Yuan F et al (1998) Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci USA 95:4607–4612CrossRef
10.
Zurück zum Zitat Otagiri M, Chuang VTG (2016) Albumin in medicine: pathological and clinical applications. Springer, SingaporeCrossRef Otagiri M, Chuang VTG (2016) Albumin in medicine: pathological and clinical applications. Springer, SingaporeCrossRef
11.
Zurück zum Zitat Langer K, Balthasar S, Vogel V et al (2003) Optimization of the preparation process for human serum albumin (HSA) nanoparticles. Int J Pharm 257:169–180CrossRef Langer K, Balthasar S, Vogel V et al (2003) Optimization of the preparation process for human serum albumin (HSA) nanoparticles. Int J Pharm 257:169–180CrossRef
17.
Zurück zum Zitat Merodio M, Arnedo A, Renedo MJ, Irache JM (2001) Ganciclovir-loaded albumin nanoparticles: characterization and in vitro release properties. Eur J Pharm Sci 12:251–259CrossRef Merodio M, Arnedo A, Renedo MJ, Irache JM (2001) Ganciclovir-loaded albumin nanoparticles: characterization and in vitro release properties. Eur J Pharm Sci 12:251–259CrossRef
24.
Zurück zum Zitat Chen RF (1967) Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem 242:173–181PubMed Chen RF (1967) Removal of fatty acids from serum albumin by charcoal treatment. J Biol Chem 242:173–181PubMed
25.
Zurück zum Zitat Suzuki I, Iigo M, Ishikawa C et al (1997) Inhibitory effects of oleic and docosahexaenoic acids on lung metastasis by colon-carcinoma-26 cells are associated with reduced matrix metalloproteinase-2 and -9 activities. Int J Cancer 73:607–612CrossRef Suzuki I, Iigo M, Ishikawa C et al (1997) Inhibitory effects of oleic and docosahexaenoic acids on lung metastasis by colon-carcinoma-26 cells are associated with reduced matrix metalloproteinase-2 and -9 activities. Int J Cancer 73:607–612CrossRef
35.
Zurück zum Zitat Polyak A, Palade EA, Balogh L et al (2011) In vitro and biodistribution examinations of Tc-99m-labelled doxorubicin-loaded nanoparticles. Nucl Med Rev Cent East Eur 14:55–62CrossRef Polyak A, Palade EA, Balogh L et al (2011) In vitro and biodistribution examinations of Tc-99m-labelled doxorubicin-loaded nanoparticles. Nucl Med Rev Cent East Eur 14:55–62CrossRef
37.
Zurück zum Zitat Soma CE, Dubernet C, Barratt G et al (2000) Investigation of the role of macrophages on the cytotoxicity of doxorubicin and doxorubicin-loaded nanoparticles on M5076 cells in vitro. J Control Release 68:283–289CrossRef Soma CE, Dubernet C, Barratt G et al (2000) Investigation of the role of macrophages on the cytotoxicity of doxorubicin and doxorubicin-loaded nanoparticles on M5076 cells in vitro. J Control Release 68:283–289CrossRef
Metadaten
Titel
Investigation of anti-tumor effect of doxorubicin-loaded human serum albumin nanoparticles prepared by a desolvation technique
verfasst von
Kotaro Kimura
Keishi Yamasaki
Koji Nishi
Kazuaki Taguchi
Masaki Otagiri
Publikationsdatum
10.04.2019
Verlag
Springer Berlin Heidelberg
Erschienen in
Cancer Chemotherapy and Pharmacology / Ausgabe 6/2019
Print ISSN: 0344-5704
Elektronische ISSN: 1432-0843
DOI
https://doi.org/10.1007/s00280-019-03832-3

Weitere Artikel der Ausgabe 6/2019

Cancer Chemotherapy and Pharmacology 6/2019 Zur Ausgabe

Update Onkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.