The online version of this article (doi:10.1186/1476-9255-9-27) contains supplementary material, which is available to authorized users.
Katerina M Antoniou, George A Margaritopoulos contributed equally to this work.
The author(s) declare that they have no competing interests.
KMA and GAM designed the study, performed the statistical analysis and wrote the manuscript. AP and KK contributed to patients recruitment and evaluation. IL and GS carried out the RT-PCRs. MCK and HAP obtained the posterior iliac crest aspirates. DAS and NMS coordinated the study and helped to draft the manuscript. All read and approved the final version of the manuscript.
Idiopathic Pulmonary Fibrosis and Rheumatoid Arthritis associated usual interstitial pneumonia seem to have the same poor outcome as there is not an effective treatment. The aim of the study is to explore the reparative ability of bone marrow mesenchymal stem cells by evaluating the system telomerase/telomeres and propose a novel therapeutic approach.
BM-MSCs were studied in 6 IPF patients, 7 patients with RA-UIP and 6 healthy controls. We evaluated the telomere length as well as the mRNA expression of both components of telomerase (human telomerase reverse transcriptase, h-TERT and RNA template complementary to the telomeric loss DNA, h-TERC).
We found that BM-MSCs from IPF, RA-UIP cases do not present smaller telomere length than the controls (p = 0.170). There was no significant difference regarding the expression of both h-TERT and h-TERC genes between patients and healthy controls (p = 0.107 and p = 0.634 respectively).
We demonstrated same telomere length and telomerase expression in BM-MSCs of both IPF and RA-UIP which could explain similarities in pathogenesis and prognosis. Maintenance of telomere length in these cells could have future implication in cell replacement treatment with stem cells of these devastating lung disorders.
Authors’ original file for figure 112950_2011_235_MOESM1_ESM.pdf
Taniguchi H, Ebina M, Kondoh Y, Ogura T, Azuma A, Suga M, Taguchi Y, Takahashi H, Nakata K, Sato A, Takeuchi M, Raghu G, Kudoh S, Nukiwa T: Pirfenidone Clinical Study Group in Japan. Pirfenidone in idiopathic pulmonary fibrosis. Eur Respir J. 2010, 35: 821-829. 10.1183/09031936.00005209. CrossRefPubMed
Hare JM, Traverse JH, Henry TD, Dib N, Strumpf RK, Schulman SP: A randomized, double-blind, placebo-controlled, dose-escalation study of intravenous adult human mesenchymal stem cells (prochymal) after acute myocardial infarction. J Am Coll Cardiol. 2009, 54: 2277-2286. 10.1016/j.jacc.2009.06.055. PubMedCentralCrossRefPubMed
Loebinger MR, Aguilar S, Janes SM: Therapeutic potential of stem cells in lung disease: progress and pitfalls. Clin Sci (Lond). 2008, 114: 99-108. 10.1042/CS20070073. CrossRef
Kastrinaki MC, Sidiropoulos P, Roche S, Ringe J, Lehmann S, Kritikos H, Vlahava VM, Delorme B, Eliopoulos GD, Jorgensen C, Charbord P, Häupl T, Boumpas DT, Papadaki HA: Functional, molecular and proteomic characterization of bone marrow mesenchymal stem cells in rheumatoid arthritis. Ann Rheum Dis. 2008, 67: 741-749. 10.1136/ard.2007.076174. CrossRefPubMed
In’t Anker PS, Noort WA, Scherjon SA, der KC Kleijburg-van, Kruisselbrink AB, van Bezooijen RL: Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential. Haematologica. 2003, 88: 845-852.
Delorme B, Charbord P: Culture and characterisation of human bone marrow mesenchymal stem cells. Methods in molecular medicine, 2nd edn: tissue engineering. Edited by: Hauser H, Fussenegger M. 2007, Humana Press Inc, Totowa, New Jersey, USA
Armanios M: Syndromes of telomere shortening. Ann Rev Genomics Hum Genet. 2009, 10: 45-61. 10.1146/annurev-genom-082908-150046. CrossRef
Ortiz LA, Gambelli F, McBride C, Gaupp D, Baddoo M, Kaminski N, Phinney DJ: Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc Natl Acad Sci USA. 2003, 100: 8407-8411. 10.1073/pnas.1432929100. PubMedCentralCrossRefPubMed
Alder JK, Chen JJ, Lancaster L, Danoff S, Su SC, Cogan JD, Vulto I, Xie M, Qi X, Tuder RM, Phillips JA, Lansdorp PM, Loyd JE, Armanios MY: Short telomeres are a risk factor for idiopathic pulmonary fibrosis. Proc Natl Acad Sci USA. 2008, 105: 13051-13056. 10.1073/pnas.0804280105. PubMedCentralCrossRefPubMed
Passegue E, Jamieson CH, Ailles LE, Weissman IL: Normal and leukemic hematopoiesis: are leukemias a stem cell disorder or a reacquisition of stem cell characteristics?. Proc Natl Acad Sci U S A 100. 2003, 1: 11842-11849. CrossRef
- Investigation of Telomerase/Telomeres system in Bone Marrow Mesenchymal Stem Cells derived from IPF and RA-UIP
Katerina M Antoniou
George A Margaritopoulos
Maria Christina Kastrinaki
Demetrios A Spandidos
Helen A Papadaki
Nikos M Siafakas
- BioMed Central
Neu im Fachgebiet Innere Medizin
Meistgelesene Bücher aus der Inneren Medizin
Mail Icon II