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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Journal of Inflammation 1/2012

Investigation of Telomerase/Telomeres system in Bone Marrow Mesenchymal Stem Cells derived from IPF and RA-UIP

Zeitschrift:
Journal of Inflammation > Ausgabe 1/2012
Autoren:
Katerina M Antoniou, George A Margaritopoulos, Athanasia Proklou, Konstantinos Karagiannis, Ismini Lasithiotaki, Giannoula Soufla, Maria Christina Kastrinaki, Demetrios A Spandidos, Helen A Papadaki, Nikos M Siafakas
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1476-9255-9-27) contains supplementary material, which is available to authorized users.
Katerina M Antoniou, George A Margaritopoulos contributed equally to this work.

Competing interests

The author(s) declare that they have no competing interests.

Authors’ contribution

KMA and GAM designed the study, performed the statistical analysis and wrote the manuscript. AP and KK contributed to patients recruitment and evaluation. IL and GS carried out the RT-PCRs. MCK and HAP obtained the posterior iliac crest aspirates. DAS and NMS coordinated the study and helped to draft the manuscript. All read and approved the final version of the manuscript.

Abstract

Objective

Idiopathic Pulmonary Fibrosis and Rheumatoid Arthritis associated usual interstitial pneumonia seem to have the same poor outcome as there is not an effective treatment. The aim of the study is to explore the reparative ability of bone marrow mesenchymal stem cells by evaluating the system telomerase/telomeres and propose a novel therapeutic approach.

Methods

BM-MSCs were studied in 6 IPF patients, 7 patients with RA-UIP and 6 healthy controls. We evaluated the telomere length as well as the mRNA expression of both components of telomerase (human telomerase reverse transcriptase, h-TERT and RNA template complementary to the telomeric loss DNA, h-TERC).

Results

We found that BM-MSCs from IPF, RA-UIP cases do not present smaller telomere length than the controls (p = 0.170). There was no significant difference regarding the expression of both h-TERT and h-TERC genes between patients and healthy controls (p = 0.107 and p = 0.634 respectively).

Conclusions

We demonstrated same telomere length and telomerase expression in BM-MSCs of both IPF and RA-UIP which could explain similarities in pathogenesis and prognosis. Maintenance of telomere length in these cells could have future implication in cell replacement treatment with stem cells of these devastating lung disorders.
Zusatzmaterial
Authors’ original file for figure 1
12950_2011_235_MOESM1_ESM.pdf
Literatur
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