Electronic supplementary material
The online version of this article (doi:10.1186/1475-2867-12-26) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
BW: Was responsible for the conception and design, testing and data acquisition, analysis and data interpretation and drafted the manuscript. BJ: made contribution to the study of caspase activities. SI: made contribution to cell culture and the study of the cytotoxicity and the DNA fragmentation. GK: made substantial contribution to conception and revised it critically for Important intellectual content. CGL:made substantial contribution to conception and revised it critically for important intellectual content. All authors read and approved the final manuscript.
The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells.
We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activities.
The inhibition of lymphoblastoid cell proliferation was noted from 8.5 μg/ml of DGA. The induction of apoptosis was confirmed by DNA fragmentation and PARP cleavage. We have demonstrated that DGA induces apoptosis by activating the caspase-8 extrinsic pathway. Caspase-3 was also activated in a dose dependent manner.
In summary, DGA exhibited an apoptosis inductor effect in TK6 cells revealing thus its potential as a cancer-preventive agent.