Thrombus formation at the interface between connectors and tubes is a potential risk factor for complications. We investigated time-dependent relationships between formation of thrombus and hemodynamic factors at the interface between connectors and tubes using optical coherence tomography (OCT) under pulsatile flow. A swept-source OCT with the center wavelength of 1330 nm was employed. The sequential process of thrombus formation at the interface of connectors and tubes in the inlet and outlet was investigated. Connectors with and without tapers were tested using identical 50-ml air-contactless circuits. Fresh human blood from healthy volunteers was circulated under pulsatile flow. Thrombus initially formed at the interface between the connector tip and the tube. Geometries of thrombus growth were different between the 2 connectors, and between the inlet and the outlet. Growth of thrombus was observed at the interface between the connectors and tubes over time in 60 min circulation, except at the outlet part of connector without tapers. At the connector without tapers outlet, thrombus propagation length from the connector edge toward the flow downstream was comparable at 10 and 60 min (0.55 ± 0.35 vs. 0.51 ± 0.32 mm, p = 0.83). Analysis using particle image velocimetry showed the presence of a flow reattachment point 1.5 mm downstream from the connector edge. These results suggest that the flow reattachment point inhibits downstream thrombus growth. We quantitatively demonstrated sequential thrombus process at the interface between the connectors and tubes under pulsatile flow of human blood using OCT.
David T, Thomas S, Walker PG. Platelet deposition in stagnation point flow: an analytical and computational simulation. Med Eng Phys. 2001;23:229–312. CrossRef
Armaly BF, Durst F, Pereira JCF, Schonung B. Experimental and theoretical investigation of backward-facing step flow. J Fluid Mech. 1983;127:473–96. CrossRef
Tamagawa M, Matsuo S. Predictions of thrombus formation using Lattice Boltzmann method (modeling of adhesion force for particles to wall). JSME. 2004;47:1027–34.
Casa LDC, Ku ND. High shear thrombus formation under pulsatile and steady flow. Cardiovasc Eng Technol. 2014;5:154–63. CrossRef
van Breugel HH, Sixma JJ, Heethaar RM. Effects of flow pulsatility on platelet adhesion to subendothelium. Arteriosclerosis. 1998;8:332–5. CrossRef
Taylor JO, Witmer KP, Neuberger T, Craven BA, Meyer RS, Deutsch S, Manning KB. In vitro quantification of time dependent thrombus size using magnetic resonance imaging and computational simulations of thrombus surface shear stresses. J Biomech Eng. 2014;136:071012. CrossRef
Otsu N. A Threshold selection method from gray-level histograms. IEEE Trans Syst Man Cybern. 1979;9:62–6. CrossRef
- Investigation of the influence of fluid dynamics on thrombus growth at the interface between a connector and tube
- Springer Japan
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