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Erschienen in: Brain Structure and Function 2/2004

01.05.2004 | Original Article

Involvement of p27(KIP1) in proliferation of the retinal pigment epithelium and ciliary body

verfasst von: Kazuhiko Yoshida, Keiko Nakayama, Satoru Kase, Hiroyasu Nagahama, Takayuki Harada, Hiromi Ikeda, Chikako Harada, Junko Imaki, Kazuhiro Ohgami, Kenji Shiratori, Shigeaki Ohno, Shinzo Nishi, Keiichi I. Nakayama

Erschienen in: Brain Structure and Function | Ausgabe 2/2004

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Abstract

According to observations in various cell lines, elimination of the cyclin-dependent kinase inhibitor p27(KIP1) during the late G1 phase of the cell cycle is required for progression to the S phase. Eyes from C57BL/6 mice at embryonic days 13, 14, and 18, and at 4 weeks of age, were analyzed by a bromodeoxyuridine cell proliferation assay and by immunocytochemistry using anti-p27(KIP1) antibody. On embryonic days 14 and 18, p27(KIP1) was detected in the ciliary body. This protein also was detected in the nuclei of the many cells of the retinal pigment epithelium on embryonic day 18, and was present in all such cells at 4 weeks of age. When p27(KIP1)-/- knockout and control mice were injected with bromodeoxyuridine between postnatal days 7 and 10 and analyzed on day 11, positive cells were abundant in the retinal pigment epithelium and the ciliary body of p27(KIP1)-/- mice, whereas few cells were positive in control mice. By fluorescent nuclear staining in whole mounts of retinal pigment epithelium at 12 weeks of age, more nuclei were present in p27(KIP1)-/- than in the wild-type mice. These results suggest that p27(KIP1) was involved in regulation of proliferation in the RPE and the ciliary body.
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Metadaten
Titel
Involvement of p27(KIP1) in proliferation of the retinal pigment epithelium and ciliary body
verfasst von
Kazuhiko Yoshida
Keiko Nakayama
Satoru Kase
Hiroyasu Nagahama
Takayuki Harada
Hiromi Ikeda
Chikako Harada
Junko Imaki
Kazuhiro Ohgami
Kenji Shiratori
Shigeaki Ohno
Shinzo Nishi
Keiichi I. Nakayama
Publikationsdatum
01.05.2004
Verlag
Springer-Verlag
Erschienen in
Brain Structure and Function / Ausgabe 2/2004
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI
https://doi.org/10.1007/s00429-004-0382-5

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