Hirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe.
In this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing.
The gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD50 value of 1.0 × 105.9 TCID50/fish.
In conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.
Kimura T, Yoshimizu M, Gorie S. A new rhabdovirus isolated in Japan from cultured hirame (Japanese flounder) Paralichthys olivaceus and ayu Plecoglossus altivelis. Dis Aquat Organ. 1986;1:209–17. CrossRef
Oh MJ, Choi TJ. A new rhabdovirus (HRV-like) isolated in Korea from cultured Japanese flounder Paralichthys olivaceus. J Fish Pathol. 1998;11:129–36.
Joung IE, Myung JH, Sung JJ, Song YH, Tae JC. The protective effect of recombinant glycoprotein vaccine against HIRRV infection. Fish Pathol. 2001;36:67–72. CrossRef
Reed LJ, Muench H. A simple method of estimating fifty percent endpoints. Am J Epidemiol. 1938;27:493–7. CrossRef
Tang X, Li Z, Zhan W. Isolation and characterization of pathogenic Listonella anguillarum of diseased half-smooth tongue sole (Cynoglossus semilaevis günther). J Ocean U China. 2008;7:343–51. CrossRef
Nishizawa T, Mori KI, Nakai T, Furusawa I, Muroga K. Polymerase chain reaction (PCR) amplification of RNA striped jack nervous necrosis virus (SJNNV). Dis Aquat Organ. 1994;18:103–7. CrossRef
Sano M, Ito T, Matsuyama T, Nakayasu C, Kurita J. Effect of water temperature shifting on mortality of Japanese flounder Paralichthys olivaceus experimentally infected with viral hemorrhagic septicemia virus. Aquaculture. 2009;286:254–8. CrossRef
Oseko N, Yoshimizu M, Kimura T. Effect of water temperature on artificial infection of rhabdovirus olivaceus (hirame rhabdovirus: HRV) to hirame (Japanese flounder, paralichthys olivaceus). Fish Pathol. 1988;23:125–32. CrossRef
Ji Y, Kim KH, Kim SG, Oh MJ, Nam SW, Kim YT. Protection of flounder against hirame rhabdovirus (HIRRV) with a DNA vaccine containing the glycoprotein gene. Vaccine. 2006;24:1009–15. CrossRef
Nishizawa T, Yoshimizu M, Winton J. Characterization of structural proteins of hirame rhabdovirus, HRV. Dis Aquat Organ. 1991;10:167–72. CrossRef
- Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China
- BioMed Central
Neu im Fachgebiet Innere Medizin
Meistgelesene Bücher aus der Inneren Medizin
Mail Icon II