Skip to main content
Erschienen in: Journal of Parasitic Diseases 1/2017

07.04.2016 | Original Article

Isolation and identification of cutaneous leishmaniasis species by PCR–RFLP in Ilam province, the west of Iran

verfasst von: Ali Kermanjani, Lame Akhlaghi, Hormozd Oormazdi, Ramtin Hadighi

Erschienen in: Journal of Parasitic Diseases | Ausgabe 1/2017

Einloggen, um Zugang zu erhalten

Abstract

Cutaneous leishmaniasis (CL) is one of the most common parasitic diseases and public health problems in Iran. CL is endemic in most parts of Ilam province, in the west of Iran. The distance from the center of country, the great number of divers rural areas, and lack of specialists and laboratory facilities have been the major causes of Leishmania species remaining unknown in this region. Polymerase chain reaction followed by restriction fragment length polymorphism was performed to identify the Leishmania species in 61 patients with cutaneous lesions. Eventually L. major was confirmed as the cause of cutaneous leishmaniasis in Ilam province, the west of Iran.
Literatur
Zurück zum Zitat Al-Jawabreh A, Schönian G, Hamarsheh O, Presber W (2006) Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears. Acta Trop 99(1):55–61CrossRefPubMed Al-Jawabreh A, Schönian G, Hamarsheh O, Presber W (2006) Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears. Acta Trop 99(1):55–61CrossRefPubMed
Zurück zum Zitat Andresen K, Gaafar A, El-Hasan A, Ismail A, Dafaila M, Theander TG, Kharazmi A (1996) Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions. Trans R Soc Trop Med Hyg 90:133–135CrossRefPubMed Andresen K, Gaafar A, El-Hasan A, Ismail A, Dafaila M, Theander TG, Kharazmi A (1996) Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: a comparison with direct microscopy of smears and sections from lesions. Trans R Soc Trop Med Hyg 90:133–135CrossRefPubMed
Zurück zum Zitat Ardehali S, Moattari A, Hatam GR, Hosseini SMH, Sharifi I (2000) Characterization of Leishmania isolated in Iran: 1. Serotyping with species specific monoclonal antibodies. Acta Trop 75(3):301–307CrossRefPubMed Ardehali S, Moattari A, Hatam GR, Hosseini SMH, Sharifi I (2000) Characterization of Leishmania isolated in Iran: 1. Serotyping with species specific monoclonal antibodies. Acta Trop 75(3):301–307CrossRefPubMed
Zurück zum Zitat Aviles H, Belli A, Armijos R, Monroy F, Harris E (1999) PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J Parasitol 85:181–187CrossRefPubMed Aviles H, Belli A, Armijos R, Monroy F, Harris E (1999) PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J Parasitol 85:181–187CrossRefPubMed
Zurück zum Zitat Daliva AM, Momen H (2002) Internal-trascribed-spacer (ITS) sequences used to explore polygenetic relationships within Leishmania. Ann Trop Med Parasitol 94(6):651–654 Daliva AM, Momen H (2002) Internal-trascribed-spacer (ITS) sequences used to explore polygenetic relationships within Leishmania. Ann Trop Med Parasitol 94(6):651–654
Zurück zum Zitat Doudi M, Hejazi H, Razavi M, Narimani M, Khanjani S, Eslami G (2010) Comparative molecular epidemiology of Leishmania major and Leishmania tropica by PCR-RFLP technique in hyper endemic cities of Isfahan and Bam, Iran. Med Sci Monit 16(11):CR530–CR535PubMed Doudi M, Hejazi H, Razavi M, Narimani M, Khanjani S, Eslami G (2010) Comparative molecular epidemiology of Leishmania major and Leishmania tropica by PCR-RFLP technique in hyper endemic cities of Isfahan and Bam, Iran. Med Sci Monit 16(11):CR530–CR535PubMed
Zurück zum Zitat Evans D (1989) Handbook on isolation, characterization and cryopreservation of Leishmania. World Health Organization, Geneva Evans D (1989) Handbook on isolation, characterization and cryopreservation of Leishmania. World Health Organization, Geneva
Zurück zum Zitat Hajjaran H, Mohebali M, Razavi MR et al (2004) Identification of Leishmania species isolated from human cutaneous leishmaniasis, using random amplified polymorphic DNA (RAPD-PCR). Iran J Publ Health 33:8–15 Hajjaran H, Mohebali M, Razavi MR et al (2004) Identification of Leishmania species isolated from human cutaneous leishmaniasis, using random amplified polymorphic DNA (RAPD-PCR). Iran J Publ Health 33:8–15
Zurück zum Zitat Kassiri H, Sharifinia N, Jalilian M, Shemshad K (2012) Epidemiological aspects of cutaneous leishmaniasis in Ilam province, west of Iran (2000–2007). Asian Pac J Trop Dis 2(Suppl 1):382–386CrossRef Kassiri H, Sharifinia N, Jalilian M, Shemshad K (2012) Epidemiological aspects of cutaneous leishmaniasis in Ilam province, west of Iran (2000–2007). Asian Pac J Trop Dis 2(Suppl 1):382–386CrossRef
Zurück zum Zitat Marfurt J, Nasereddin A, Niederwieser I et al (2003a) Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. J Clin Microbiol 41:3147–3153CrossRefPubMedPubMedCentral Marfurt J, Nasereddin A, Niederwieser I et al (2003a) Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. J Clin Microbiol 41:3147–3153CrossRefPubMedPubMedCentral
Zurück zum Zitat Marfurt J, Niederwieser I, Makia ND, Beck HP, Felger I (2003b) Diagnostic genotyping of Old and New World Leishmania species by PCR-RFLP. Diagn Microbiol Infect Dis 46:115–124CrossRefPubMed Marfurt J, Niederwieser I, Makia ND, Beck HP, Felger I (2003b) Diagnostic genotyping of Old and New World Leishmania species by PCR-RFLP. Diagn Microbiol Infect Dis 46:115–124CrossRefPubMed
Zurück zum Zitat Nadim A (2000) Leishmaniasis. In: Azizi F, Janghorbani M, Hatam H (eds) Epidemiology and control of common disorders in Iran, 2nd edn. Metabolism Research Center, Shaheed Beheshti University of Medical Sciences, Tehran, pp 524–534 Nadim A (2000) Leishmaniasis. In: Azizi F, Janghorbani M, Hatam H (eds) Epidemiology and control of common disorders in Iran, 2nd edn. Metabolism Research Center, Shaheed Beheshti University of Medical Sciences, Tehran, pp 524–534
Zurück zum Zitat Reithinger R, Dujardin JC (2007) Molecular diagnosis of leishmaniasis: current status and future applications. J Clin Microbiol 45(1):21–25CrossRefPubMed Reithinger R, Dujardin JC (2007) Molecular diagnosis of leishmaniasis: current status and future applications. J Clin Microbiol 45(1):21–25CrossRefPubMed
Zurück zum Zitat Rotureau B, Ravel C, Couppié P et al (2006) Use of PCR-restriction fragment length polymorphism analysis to identify the main new world Leishmania species and analyze their taxonomic properties and polymorphism by application of the assay to clinical samples. J Clin Microbiol 44:459–467CrossRefPubMedPubMedCentral Rotureau B, Ravel C, Couppié P et al (2006) Use of PCR-restriction fragment length polymorphism analysis to identify the main new world Leishmania species and analyze their taxonomic properties and polymorphism by application of the assay to clinical samples. J Clin Microbiol 44:459–467CrossRefPubMedPubMedCentral
Zurück zum Zitat Schönian G, Nasereddin A, Dinse N et al (2003) PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47:349–358CrossRefPubMed Schönian G, Nasereddin A, Dinse N et al (2003) PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47:349–358CrossRefPubMed
Zurück zum Zitat Singh S, Sivakumar R (2003) Recent advances in the diagnosis of leishmaniasis. J Postgrad Med 49:55–60CrossRefPubMed Singh S, Sivakumar R (2003) Recent advances in the diagnosis of leishmaniasis. J Postgrad Med 49:55–60CrossRefPubMed
Zurück zum Zitat Tashakori M, Ajdary S, Kariminia A et al (2003) Characterization of Leishmania Species and L. major strains in different endemic areas of leishmaniasis in Iran. Iran Biomed J 7:43–50 Tashakori M, Ajdary S, Kariminia A et al (2003) Characterization of Leishmania Species and L. major strains in different endemic areas of leishmaniasis in Iran. Iran Biomed J 7:43–50
Zurück zum Zitat Tavares CA, Fernandes AP, Melo MN (2003) Molecular diagnosis of leishmaniasis. Expert Rev Mol Diagn 3:657–667CrossRefPubMed Tavares CA, Fernandes AP, Melo MN (2003) Molecular diagnosis of leishmaniasis. Expert Rev Mol Diagn 3:657–667CrossRefPubMed
Zurück zum Zitat Yaghoobi-Ershadi MR, Hanafi-Bojd AA, Javadian E, Jafari R, Zahraei-Ramazani AR, Mohebali M (2002) A new focus of cutaneous leishmaniasis caused by Leishmania tropica. Saudi Med J 23:291–294PubMed Yaghoobi-Ershadi MR, Hanafi-Bojd AA, Javadian E, Jafari R, Zahraei-Ramazani AR, Mohebali M (2002) A new focus of cutaneous leishmaniasis caused by Leishmania tropica. Saudi Med J 23:291–294PubMed
Zurück zum Zitat Yazdanpanah H, Rostamianpur M (2013) Analysis of spatial distribution of leishmaniasis and its relationship with climatic parameters (case study: Ilam Province). Bull Environ Pharmacol Life Sci 2(12):80–86 Yazdanpanah H, Rostamianpur M (2013) Analysis of spatial distribution of leishmaniasis and its relationship with climatic parameters (case study: Ilam Province). Bull Environ Pharmacol Life Sci 2(12):80–86
Metadaten
Titel
Isolation and identification of cutaneous leishmaniasis species by PCR–RFLP in Ilam province, the west of Iran
verfasst von
Ali Kermanjani
Lame Akhlaghi
Hormozd Oormazdi
Ramtin Hadighi
Publikationsdatum
07.04.2016
Verlag
Springer India
Erschienen in
Journal of Parasitic Diseases / Ausgabe 1/2017
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-016-0772-7

Weitere Artikel der Ausgabe 1/2017

Journal of Parasitic Diseases 1/2017 Zur Ausgabe

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.