Skip to main content
Erschienen in: Journal of Mammary Gland Biology and Neoplasia 1-2/2016

05.04.2016

Isolation of Endoplasmic Reticulum Fractions from Mammary Epithelial Tissue

verfasst von: Eric Chanat, Annabelle Le Parc, Hichem Lahouassa, Bouabid Badaoui

Erschienen in: Journal of Mammary Gland Biology and Neoplasia | Ausgabe 1-2/2016

Einloggen, um Zugang zu erhalten

Abstract

In the mammary glands of lactating animals, the mammary epithelial cells that surround the lumen of the acini produce and secrete copious amounts of milk. Functional differentiation of these mammary epithelial cells depends on the development of high-efficiency secretory pathways, notably for protein and lipid secretion. Protein secretion is a fundamental process common to all animal cells that involves a subset of cellular organelles, including the endoplasmic reticulum and the Golgi apparatus. In contrast, en masse secretion of triglycerides and cholesterol esters in the form of milk fat globules is a unique feature of the mammary epithelial cell. Cytoplasmic lipid droplets, the intracellular precursors of milk fat globules, originate from the endoplasmic reticulum, as do most milk-specific proteins. This organelle is therefore pivotal in the biogenesis of milk components. Fractionation of the cell into its subcellular parts is an approach that has proven very powerful for understanding organelle function and for studying the specific role of an organelle in a given cell activity. Here we describe a method for the purification of both smooth and rough microsomes, the membrane-bound endoplasmic reticulum fragments that form from endoplasmic reticulum domains when cells are broken up, from mammary gland tissue at lactation.
Literatur
1.
Zurück zum Zitat Inman JL, Robertson C, Mott JD, Bissell MJ. Mammary gland development: cell fate specification, stem cells and the microenvironment. Development. 2015;142(6):1028–42.CrossRefPubMed Inman JL, Robertson C, Mott JD, Bissell MJ. Mammary gland development: cell fate specification, stem cells and the microenvironment. Development. 2015;142(6):1028–42.CrossRefPubMed
2.
Zurück zum Zitat Palade GE. Intracellular aspects of the process of protein synthesis. Science. 1975;189:347–58.CrossRefPubMed Palade GE. Intracellular aspects of the process of protein synthesis. Science. 1975;189:347–58.CrossRefPubMed
4.
Zurück zum Zitat Bouguyon E, Beauvallet C, Huet JC, Chanat E. Disulphide bonds in casein micelle from milk. Biochem Biophys Res Commun. 2006;343(2):450–8.CrossRefPubMed Bouguyon E, Beauvallet C, Huet JC, Chanat E. Disulphide bonds in casein micelle from milk. Biochem Biophys Res Commun. 2006;343(2):450–8.CrossRefPubMed
7.
Zurück zum Zitat Holt C, Carver JA, Ecroyd H, Thorn DC. Invited review: Caseins and the casein micelle: their biological functions, structures, and behavior in foods. J Dairy Sci. 2013;96(10):6127–46.CrossRefPubMed Holt C, Carver JA, Ecroyd H, Thorn DC. Invited review: Caseins and the casein micelle: their biological functions, structures, and behavior in foods. J Dairy Sci. 2013;96(10):6127–46.CrossRefPubMed
8.
Zurück zum Zitat Turner MD, Rennison ME, Handel SE, Wilde CJ, Burgoyne RD. Proteins are secreted by both constitutive and regulated secretory pathways in lactating mouse mammary epithelial cells. J Cell Biol. 1992;117(2):269–78.CrossRefPubMed Turner MD, Rennison ME, Handel SE, Wilde CJ, Burgoyne RD. Proteins are secreted by both constitutive and regulated secretory pathways in lactating mouse mammary epithelial cells. J Cell Biol. 1992;117(2):269–78.CrossRefPubMed
9.
Zurück zum Zitat Franke WW, Luder MR, Kartenbeck J, Zerban H, Keenan TW. Involvement of vesicle coat material in casein secretion and surface regeneration. J Cell Biol. 1976;69(1):173–95.CrossRefPubMed Franke WW, Luder MR, Kartenbeck J, Zerban H, Keenan TW. Involvement of vesicle coat material in casein secretion and surface regeneration. J Cell Biol. 1976;69(1):173–95.CrossRefPubMed
10.
Zurück zum Zitat Heid HW, Keenan TW. Intracellular origin and secretion of milk fat globules. Eur J Cell Biol. 2005;84(2–3):245–58.CrossRefPubMed Heid HW, Keenan TW. Intracellular origin and secretion of milk fat globules. Eur J Cell Biol. 2005;84(2–3):245–58.CrossRefPubMed
11.
Zurück zum Zitat Mather IH, Keenan TW. Origin and secretion of milk lipids. J Mammary Gland Biol Neoplasia. 1998;3(3):259–73.CrossRefPubMed Mather IH, Keenan TW. Origin and secretion of milk lipids. J Mammary Gland Biol Neoplasia. 1998;3(3):259–73.CrossRefPubMed
15.
Zurück zum Zitat Lavoie C, Lanoix J, Kan FW, Paiement J. Cell-free assembly of rough and smooth endoplasmic reticulum. J Cell Sci. 1996;109(Pt 6):1415–25.PubMed Lavoie C, Lanoix J, Kan FW, Paiement J. Cell-free assembly of rough and smooth endoplasmic reticulum. J Cell Sci. 1996;109(Pt 6):1415–25.PubMed
16.
Zurück zum Zitat Paiement J, Bergeron JJ. Localization of GTP-stimulated core glycosylation to fused microsomes. J Cell Biol. 1983;96(6):1791–6.CrossRefPubMed Paiement J, Bergeron JJ. Localization of GTP-stimulated core glycosylation to fused microsomes. J Cell Biol. 1983;96(6):1791–6.CrossRefPubMed
18.
Zurück zum Zitat Keenan TW, Huang CM, Morre DJ. Membranes of mammary gland. V. Isolation of Golgi apparatus and rough endoplasmic reticulum from bovine mammary gland. J Dairy Sci. 1972;55(11):1577–85.CrossRefPubMed Keenan TW, Huang CM, Morre DJ. Membranes of mammary gland. V. Isolation of Golgi apparatus and rough endoplasmic reticulum from bovine mammary gland. J Dairy Sci. 1972;55(11):1577–85.CrossRefPubMed
19.
Zurück zum Zitat Paiement J, Young R, Roy L, Bergeron JJ. Isolation of rough and smooth membrane domains of the endoplasmic reticulum from rat liver. In: Celis J, editor. Cell biology: a laboratory handbook. 3rd ed. 2004. p. 41–4. Paiement J, Young R, Roy L, Bergeron JJ. Isolation of rough and smooth membrane domains of the endoplasmic reticulum from rat liver. In: Celis J, editor. Cell biology: a laboratory handbook. 3rd ed. 2004. p. 41–4.
Metadaten
Titel
Isolation of Endoplasmic Reticulum Fractions from Mammary Epithelial Tissue
verfasst von
Eric Chanat
Annabelle Le Parc
Hichem Lahouassa
Bouabid Badaoui
Publikationsdatum
05.04.2016
Verlag
Springer US
Erschienen in
Journal of Mammary Gland Biology and Neoplasia / Ausgabe 1-2/2016
Print ISSN: 1083-3021
Elektronische ISSN: 1573-7039
DOI
https://doi.org/10.1007/s10911-016-9351-x

Weitere Artikel der Ausgabe 1-2/2016

Journal of Mammary Gland Biology and Neoplasia 1-2/2016 Zur Ausgabe

Update Onkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.