nach oben

Breast Cancer Research

Erschienen in:

01.08.2009 | Meeting report

It's all in the details: methods in breast development and cancer

verfasst von: Mohamed Bentires-Alj, Robert B Clarke, Jos Jonkers, Matthew Smalley, Torsten Stein

Erschienen in: Breast Cancer Research | Ausgabe 4/2009

Einloggen, um Zugang zu erhalten

Abstract

The inaugural European Network for Breast Development and Cancer (ENBDC) meeting on 'Methods in Mammary Gland Development and Cancer' was held in Weggis, Switzerland last April. The goal was to discuss the details of techniques used to study mammary gland biology and tumourigenesis. Highlights of this meeting included the use of four-colour fluorescence for protein co-localisation in tissue microarrays, genome analysis at single cell resolution, technical issues in the isolation of normal and tumour stem cells, and the use of mouse models and mammary gland transplantations to elucidate gene function in mammary development and to study drug resistance in breast cancer.

Robertson D, Savage K, Reis-Filho JS, Isacke CM: Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue. BMC Cell Biol. 2008, 9: 13-10.1186/1471-2121-9-13.CrossRefPubMedPubMedCentral

European Network of Breast Development and Cancer Labs. [http://www.enbdc.org]

Jensen KB, Watt FM: Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence. Proc Natl Acad Sci USA. 2006, 103: 11958-11963. 10.1073/pnas.0601886103.CrossRefPubMedPubMedCentral

Jensen KB, Collins CA, Nascimento E, Tan DW, Frye M, Itami S, Watt FM: Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis. Cell Stem Cell. 2009, 4: 427-439. 10.1016/j.stem.2009.04.014.CrossRefPubMedPubMedCentral

Charafe-Jauffret E, Ginestier C, Iovino F, Wicinski J, Cervera N, Finetti P, Hur MH, Diebel ME, Monville F, Dutcher J, Brown M, Viens P, Xerri L, Bertucci F, Stassi G, Dontu G, Birnbaum D, Wicha MS: Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature. Cancer Res. 2009, 69: 1302-1313. 10.1158/0008-5472.CAN-08-2741.CrossRefPubMedPubMedCentral

Quintana E, Shackleton M, Sabel MS, Fullen DR, Johnson TM, Morrison SJ: Efficient tumour formation by single human melanoma cells. Nature. 2008, 456: 593-598. 10.1038/nature07567.CrossRefPubMedPubMedCentral

Liu X, Holstege H, Gulden van der H, Treur-Mulder M, Zevenhoven J, Velds A, Kerkhoven RM, van Vliet MH, Wessels LF, Peterse JL, Berns A, Jonkers J: Somatic loss of BRCA1 and p53 in mice induces mammary tumors with features of human BRCA1-mutated basal-like breast cancer. Proc Natl Acad Sci USA. 2007, 104: 12111-12116. 10.1073/pnas.0702969104.CrossRefPubMedPubMedCentral

Rottenberg S, Nygren AO, Pajic M, van Leeuwen FW, Heijden van der I, Wetering van de K, Liu X, de Visser KE, Gilhuijs KG, van Tellingen O, Schouten JP, Jonkers J, Borst P: Selective induction of chemotherapy resistance of mammary tumors in a conditional mouse model for hereditary breast cancer. Proc Natl Acad Sci USA. 2007, 104: 12117-12122. 10.1073/pnas.0702955104.CrossRefPubMedPubMedCentral

Rottenberg S, Jaspers JE, Kersbergen A, Burg van der E, Nygren AO, Zander SA, Derksen PW, de Bruin M, Zevenhoven J, Lau A, Boulter R, Cranston A, O'Connor MJ, Martin NM, Borst P, Jonkers J: High sensitivity of BRCA1-deficient mammary tumors to the PARP inhibitor AZD2281 alone and in combination with platinum drugs. Proc Natl Acad Sci USA. 2008, 105: 17079-17084. 10.1073/pnas.0806092105.CrossRefPubMedPubMedCentral

10.

Sleeman KE, Kendrick H, Robertson D, Isacke CM, Ashworth A, Smalley MJ: Dissociation of estrogen receptor expression and in vivo stem cell activity in the mammary gland. J Cell Biol. 2007, 176: 19-26. 10.1083/jcb.200604065.CrossRefPubMedPubMedCentral

11.

Sleeman KE, Kendrick H, Ashworth A, Isacke CM, Smalley MJ: CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells. Breast Cancer Res. 2006, 8: R7-10.1186/bcr1371.CrossRefPubMed

12.

Taddei I, Deugnier MA, Faraldo MM, Petit V, Bouvard D, Medina D, Fassler R, Thiery JP, Glukhova MA: Beta1 integrin deletion from the basal compartment of the mammary epithelium affects stem cells. Nat Cell Biol. 2008, 10: 716-722. 10.1038/ncb1734.CrossRefPubMedPubMedCentral

Titel: It's all in the details: methods in breast development and cancer
verfasst von: Mohamed Bentires-Alj
Robert B Clarke
Jos Jonkers
Matthew Smalley
Torsten Stein
Publikationsdatum: 01.08.2009
Verlag: BioMed Central
Erschienen in: Breast Cancer Research / Ausgabe 4/2009
Elektronische ISSN: 1465-542X
DOI: https://doi.org/10.1186/bcr2346

Neu im Fachgebiet Onkologie

25.04.2024 | Nierenkarzinom | Nachrichten

Springer Medizin

It's all in the details: methods in breast development and cancer

Abstract

Neu im Fachgebiet Onkologie

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Update Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2009

Tumor aromatase expression as a prognostic factor for local control in young breast cancer patients after breast-conserving treatment

Recent advances in systemic therapy. When HER2 is not the target: advances in the treatment of HER2-negative metastatic breast cancer

Genomic profiling of breast tumours in relation to BRCAabnormalities and phenotypes

Construction of an MUC-1 promoter driven, conditionally replicating adenovirus that expresses the sodium iodide symporter for gene therapy of breast cancer

Recent advances in systemic therapy: Advances in systemic therapy for HER2-positive metastatic breast cancer

MicroRNA expression profiling of male breast cancer

Neu im Fachgebiet Onkologie

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Update Onkologie