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Virchows Archiv OnlineFirst articles


Letter to the editor on Donzel M et al. “Androgenetic/biparental mosaicism in a diploid mole-like conceptus: report of a case with triple paternal contribution”

verfasst von:
Hirokazu Usui, Eri Katayama, Natsuko Nakamura, Kaori Koga

Open Access 04.12.2023 | COMMENTARY

Postponing evolution: why are we choosing to ignore the need for a digital transformation in pathology?

verfasst von:
Catarina Eloy

Open Access 28.11.2023 | ORIGINAL ARTICLE

Immunohistochemical markers as predictors of prognosis in multifocal prostate cancer

The impact of tumor focality on prostate cancer (PCa) prognosis has been addressed in several studies with conflicting results. Tumor foci from multifocal (MF) PCa can show highly heterogeneous molecular features. Our aim was to analyze the …

verfasst von:
Laura Segalés, Nuria Juanpere, Nerea Gallarín, Marta Lorenzo, David López, Júlia Perera-Bel, Alejo Rodriguez-Vida, Lluís Fumadó, Lluís Cecchini, Joaquim Bellmunt, Josep Lloreta-Trull, Silvia Hernández-Llodrà


Molecular pathology as basis for timely cancer diagnosis and therapy

Precision and personalized therapeutics have witnessed significant advancements in technology, revolutionizing the capabilities of laboratories to generate vast amounts of genetic data. Coupled with computational resources for analysis and …

verfasst von:
A. Craig Mackinnon Jr, Darshan Shimoga Chandrashekar, David I. Suster

Open Access 28.11.2023 | REVIEW AND PERSPECTIVES

Molecular pathology in breast disease: diagnostic, prognostic, and therapeutic tools

Molecular testing in breast cancer gained increasing attention and importance as specific molecular results can tailor not only oncological decisions on systemic adjuvant or neoadjuvant or in metastatic setting, but increasingly serve in …

verfasst von:
Zsuzsanna Varga, Umberto Maccio

Open Access 27.11.2023 | Original Article

Pathologic approach to Neonatal cholestasis with a simple scoring system for biliary atresia

A liver biopsy is essential for the diagnostic workup of persistent neonatal cholestasis (NC). The differential diagnosis of NC is broad, including obstructive and non-obstructive causes. In addition, histologic features of certain disorders may …

verfasst von:
Khadiga M. Ali, Khaled R. Zalata, Tarik Barakat, Sherine M. Elzeiny

Open Access 24.11.2023 | ORIGINAL ARTICLE

Glyoxal acid-free (GAF) histological fixative is a suitable alternative to formalin: results from an open-label comparative non-inferiority study

Formalin, an aqueous solution of formaldehyde, has been the gold standard for fixation of histological samples for over a century. Despite its considerable advantages, growing evidence points to objective toxicity, particularly highlighting its …

verfasst von:
Ales Ryska, Anna Sapino, Stefania Landolfi, Irene Sansano Valero, Santiago Ramon y Cajal, Pedro Oliveira, Paolo Detillo, Luca Lianas, Francesca Frexia, Pier Andrea Nicolosi, Tommaso Monti, Benedetta Bussolati, Caterina Marchiò, Gianni Bussolati

Open Access 24.11.2023 | REVIEW AND PERSPECTIVES

Genomic alterations and diagnosis of renal cancer

The application of molecular profiling has made substantial impact on the classification of urogenital tumors. Therefore, the 2022 World Health Organization incorporated the concept of molecularly defined renal tumor entities into its …

verfasst von:
Xingming Zhang, Hella A. Bolck, Niels J. Rupp, Holger Moch


The practical utility of AI-assisted molecular profiling in the diagnosis and management of cancer of unknown primary: an updated review

Cancer of unknown primary (CUP) presents a complex diagnostic challenge, characterized by metastatic tumors of unknown tissue origin and a dismal prognosis. This review delves into the emerging significance of artificial intelligence (AI) and …

verfasst von:
Shuihui Wang Lorkowski, Josephine K. Dermawan, Brian P. Rubin

Open Access 23.11.2023 | ORIGINAL ARTICLE

HER2 copy number determination in breast cancer using the highly sensitive droplet digital PCR method

Human epidermal growth factor receptor 2 (HER)-positive breast cancer (BC) is characterized by an aggressive clinical course. In the case of HER2 overexpression/amplification, patients benefit from HER2-targeting therapies. Standardized diagnostic …

verfasst von:
Beate Alinger-Scharinger, Cornelia Kronberger, Georg Hutarew, Wolfgang Hitzl, Roland Reitsamer, Klaassen-Federspiel Frederike, Martina Hager, Thorsten Fischer, Karl Sotlar, Heidi Jaksch-Bogensperger

21.11.2023 | BRIEF REPORT

Adenoid cystic carcinoma metastatic to the kidney: a series of 10 patients emphasizing unilateral presentation and long time interval from primary diagnosis

Adenoid cystic carcinoma (AdCC) metastasis to kidney is rare. We identified 10 patients with metastatic AdCC in multi-institutional collaboration. Core needle biopsy was the most common specimen (n = 6). Patients were predominately female (n = 7) …

verfasst von:
Mahmut Akgul, Jennifer Cha, Sean R. Williamson, Kanika Arora, Murat Celik, Lisa M. Rooper, Debra L. Zynger, Ankur R. Sangoi

20.11.2023 | Brief Report

T cell phenotype and lack of eosinophilia are not uncommon in extramedullary myeloid/lymphoid neoplasms with ETV6::FLT3 fusion: a case report and review of the literature

In the 2022, WHO and ICC classifications, myeloid/lymphoid neoplasms with eosinophilia (M/LN-eo) and tyrosine kinase gene fusions represent rare hematologic malignancies driven by rearrangements of PDGFRA, PDGFRB, FGFR1, JAK2, FLT3, and ETV6::ABL1 …

verfasst von:
Jérémy Schoelinck, Julie Gervasoni, Yann Guillermin, Emmanuel Beillard, Daniel Pissaloux, Catherine Chassagne-Clement

Open Access 20.11.2023 | REVIEW AND PERSPECTIVES

Molecular diagnostics tailoring personalized cancer therapy—an oncologist’s view

Medical oncology is rapidly evolving with the implementation of personalized, targeted therapies. Advances in molecular diagnostics and the biologic understanding of cancer pathophysiology led to the identification of specific genetic alterations …

verfasst von:
Jakob M. Riedl, Florian Moik, Tamara Esterl, Sarah M. Kostmann, Armin Gerger, Philipp J. Jost

16.11.2023 | Correction

Correction to: Immunoglobulin light chain transcript detection by ultrasensitive RNA in situ hybridization for B-cell lymphoma diagnosis

verfasst von:
Luisa Lorenzi, Silvia Lonardi, Michela Bonezzi, Stefania Zini, Mattia Bugatti, Arianna Valzelli, Flavia Melotti, Mattia Facchetti, Iacopo Ghini, Vincenzo Villanacci, Piera Balzarini, Marco Pizzi, Viviana Giustini, Anna Galvagni, Marco Chiarini, Angelo Paolo Dei Tos, William Vermi, Stefano Casola, Fabio Facchetti

14.11.2023 | BRIEF REPORT

Large B-cell lymphoma with IRF4 rearrangement: a multi-centric study with focus on potential misleading phenotypes

Large B-cell lymphoma with IRF4 rearrangement (LBCL-IRF4) is a rare lymphoid neoplasm, usually occurring in the pediatric/young-adult age. Despite this, subsets of cases occur in elderly patients and express CD5, possibly entering the differential …

verfasst von:
Marco Pizzi, Lucia Bongiovanni, Luisa Lorenzi, Simona Righi, Federico Scarmozzino, Piera Balzarini, Luisa Santoro, Lara Mussolin, Elisa Carraro, Marta Pillon, Laura Bonaldi, Fabrizio Vianello, Claudio Agostinelli, Maurilio Ponzoni, Angelo Paolo Dei Tos, Elena Sabattini

14.11.2023 | Brief Report

Solitary fibrous tumours involving the genitourinary tract: a case series in rare locations, highlighting the role of STAT6 immunohistochemistry

Solitary fibrous tumour (SFT) is a mesenchymal neoplasm with variable behaviour, very rarely involving the genitourinary (GU) tract. Most reported cases correspond to isolated case reports. STAT6 immunohistochemistry is a more recent and reliable …

verfasst von:
João Lobo, Lara R Harik, Charles C Peyton, Mariana Andozia Morini, Bassel Zein-Sabatto, Thomas Winokur, Valeria Dal Zotto, Cristina Magi-Galluzzi


Branchioma: immunohistochemical and molecular genetic study of 23 cases highlighting frequent loss of retinoblastoma 1 immunoexpression

Branchioma is an uncommon benign neoplasm with an adult male predominance, typically occurring in the lower neck region. Different names have been used for this entity in the past (ectopic hamartomatous thymoma, branchial anlage mixed tumor …

verfasst von:
Martina Bradová, Lester D. R. Thompson, Martin Hyrcza, Tomáš Vaněček, Petr Grossman, Michael Michal Jr., Veronika Hájková, Touraj Taheri, Niels Rupp, David Suster, Sunil Lakhani, Dimitar Hadži Nikolov, Radim Žalud, Alena Skálová, Michal Michal, Abbas Agaimy


Expression of CD5 in salivary gland tumors: an ancillary marker for carcinoma showing thymus-like differentiation (CASTLE) of the major salivary gland

Recently, cases of carcinoma showing thymus-like differentiation (CASTLE) occurring in major salivary glands have been identified. To assess the diagnostic value of CD5 immunohistochemistry in distinguishing salivary CASTLE from other types of …

verfasst von:
Eiichi Sasaki, Hoshino Terada, Naoki Oishi, Akari Iwakoshi, Katsuhiro Masago, Hirokazu Matsushita, Hidetaka Yamamoto, Nobuhiro Hanai, Hisashi Tateyama

08.11.2023 | Original Article

The value of GLI1 and p16 immunohistochemistry in the premolecular screening for GLI1-altered mesenchymal neoplasms

Mesenchymal neoplasms with GLI1 alterations have recently been reported in several anatomic locations. Their morphology and immunohistochemistry (IHC) are nonspecific, making their recognition a true challenge. To assess the diagnostic value of …

verfasst von:
Isidro Machado, Abbas Agaimy, Francisco Giner, Samuel Navarro, Michael Michal, Julia Bridge, Reyes Claramunt, José Antonio López-Guerrero, Javier Alcacer, Konstantinos Linos, Antonio Llombart-Bosch

Open Access 08.11.2023 | ORIGINAL ARTICLE

Prognostic value of HPV-PCR, p16 and p53 immunohistochemical status on local recurrence rate and survival in patients with vulvar squamous cell carcinoma

The primary aim of this study was to assess the association between human papilloma virus (HPV) and p53 expression and local recurrence (LR), disease specific survival (DSS), and overall survival (OS) in patients with vulvar squamous cell …

verfasst von:
A. W. Pouwer, N. C. te Grootenhuis, F. Hinten, G. H. de Bock, A. G. J. van der Zee, W. J. G. Melchers, M. H. M. Oonk, J. A. de Hullu, H. Hollema, J. Bulten