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Journal of Natural Medicines
Erschienen in:

24.12.2023 | Original Paper

Kaempferol-3-O-(2″-O-galloyl-β-d-glucopyranoside): a novel neuroprotective agent from Diospryros kaki against cerebral ischemia—induced brain injury

verfasst von: Loan Thanh Thi Nguyen, Xoan Thi Le, Ha Thi Nguyen, Tai Van Nguyen, Hang Nguyet Thi Pham, Anh Van Thi Pham, Kinzo Matsumoto

Erschienen in: Journal of Natural Medicines | Ausgabe 2/2024

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Abstract

Our previous study demonstrated neuroprotective and therapeutic effects of a standardized flavonoid extract from leaves of Diospyros kaki L.f. (DK) on middle cerebral artery occlusion-and-reperfusion (MCAO/R)-induced brain injury and its underlying mechanisms. This study aimed to clarify flavonoid components responsible for the effects of DK using in vitro and in vivo transient brain ischemic models. Organotypic hippocampal slice cultures (OHSCs) subjected to oxygen- and glucose-deprivation (OGD) were performed to evaluate in vitro neuroprotective activity of DK extract and nine isolated flavonoid components. MCAO/R mice were employed to elucidate in vivo neuroprotective effects of the flavonoid component that exhibited the most potent neuroprotective effect in OHSCs. DK extract and seven flavonoids [quercetin, isoquercetin, hyperoside, quercetin-3-O-(2″-O-galloyl-β-d-galactopyranoside), kaempferol, astragalin, and kaempferol-3-O-(2″-O-galloyl-β-d-glucopyranoside) compound (9)] attenuated OGD-induced neuronal cell damage and compound (9) possessed the most potent neuroprotective activity in OHSCs. The MCAO/R mice showed cerebral infarction, massive weight loss, characteristic neurological symptoms, and deterioration of neuronal cells in the brain. Compound (9) and a reference drugs, edaravone, significantly attenuated these physical and neurological impairments. Compound (9) mitigated the blood–brain barrier dysfunction and the change of glutathione and malondialdehyde content in the MCAO mouse brain. Edaravone suppressed the oxidative stress but did not significantly affect the blood–brain barrier permeability. The present results indicated that compound (9) is a flavonoid constituent of DK with a potent neuroprotective activity against transient ischemia-induced brain damage and this action, at least in part, via preservation of blood–brain barrier integrity and suppression of oxidative stress caused by ischemic insult.

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Metadaten
Titel
Kaempferol-3-O-(2″-O-galloyl-β-d-glucopyranoside): a novel neuroprotective agent from Diospryros kaki against cerebral ischemia—induced brain injury
verfasst von
Loan Thanh Thi Nguyen
Xoan Thi Le
Ha Thi Nguyen
Tai Van Nguyen
Hang Nguyet Thi Pham
Anh Van Thi Pham
Kinzo Matsumoto
Publikationsdatum
24.12.2023
Verlag
Springer Nature Singapore
Erschienen in
Journal of Natural Medicines / Ausgabe 2/2024
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI
https://doi.org/10.1007/s11418-023-01765-z

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