Knockout of Erbin promotes pyroptosis via regulating NLRP3/caspase-1/Gasdermin D pathway in sepsis-induced acute kidney injury
- 13.06.2023
- Original article
Erschienen in:
- Verfasst von
- Yuping Liu
- Qing Fang
- Tingqian Ming
- Jing Zuo
- Guoqing Jing
- Xuemin Song
- Erschienen in
- Clinical and Experimental Nephrology | Ausgabe 9/2023
Abstract
Background
This study aims to investigate the correlation between Erbin and sepsis, and the role of Erbin on the pyroptosis pathway in acute kidney injury caused by sepsis and NLRP3/caspase-1/Gasdermin D pathway.
Methods
In the study, lipopolysaccharide (LPS) treatment or cecal ligation and puncture (CLP) surgery on mice were used to stimulate the in vitro and in vivo sepsis-induced renal injury model. The male C57BL/6 of wild-type mice (WT) and Erbin-knockout mice (Erbin−/−, EKO) were randomly divided into four groups (WT + Sham, WT + CLP, EKO + Sham, EKO + CLP). Inflammatory cytokine, renal function, pyroptotic cell numbers and the levels of protein and mRNA expression of pyroptosis, including the NLRP3 (all P < 0.05), were analyzed and found increase in Erbin−/− mice with CLP and LPS-induced HK-2 cells.
Results
The inhibited of Erbin shows a renal damaged effect by promoting NLRP3 inflammasome-mediated pyroptosis in SI-AKI.
Conclusion
This study demonstrated a novel mechanism by which Erbin regulates NLRP3 inflammasome-mediated pyroptosis in SI-AKI.
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- Titel
- Knockout of Erbin promotes pyroptosis via regulating NLRP3/caspase-1/Gasdermin D pathway in sepsis-induced acute kidney injury
- Verfasst von
-
Yuping Liu
Qing Fang
Tingqian Ming
Jing Zuo
Guoqing Jing
Xuemin Song
- Publikationsdatum
- 13.06.2023
- Verlag
- Springer Nature Singapore
- Erschienen in
-
Clinical and Experimental Nephrology / Ausgabe 9/2023
Print ISSN: 1342-1751
Elektronische ISSN: 1437-7799 - DOI
- https://doi.org/10.1007/s10157-023-02364-8
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