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01.12.2014 | Original Article | Ausgabe 9/2014

Clinical Oral Investigations 9/2014

Laboratory validation of a new gas-enhanced dentine liquid permeation evaluation system

Zeitschrift:
Clinical Oral Investigations > Ausgabe 9/2014
Autoren:
Anas Al-Jadaa, Thomas Attin, Timo Peltomäki, Christian Heumann, Patrick R. Schmidlin

Abstract

Aim

To validate a new automated dentine permeability testing platform based on pressure change measurements.

Methodology

A split chamber was designed allowing for concomitant measurement of fluid permeation and pressure difference. In a first test, system reliability was assessed by interposing a solid metal disk, embedded composite resin disks, or teeth by consecutively measuring eight times under standardized conditions. Secondly, the repeatability and applicability of the method was tested in a dentine wound model by using intact third molars: Class I (2 × 5 mm) and a full occlusal preparation as well a ceramic restoration were consecutively performed and repeatedly measured eight times each. In the last test, the system detection limit as well correlation between gas pressure difference and liquid permeation were evaluated: Again, third molars were used and occlusal preparations of increasing size (2 × 5, 3 × 5, 4 × 5, and 5 × 5 mm and full occlusal preparations, respectively) were made. Data was analyzed for the linearity of measurement, and R 2 values were calculated.

Results

The embedding procedure allowed for perfect separation of the two chambers, and no significant variation in repeated measurements of evaluated samples for the respective treatments (p = 0.05) was found. The detection was 0.002 hPa/min for the pressure slope and 0.0225 μl/min for the fluid infiltration, respectively. The saline volume was highly correlating to the gas pressure changes (R 2 = 0.996, p < 0.0001).

Conclusions

The presented method is a reliable and exact tool to assess dentine permeability by nondestructive and repeatable measurements.

Clinical relevance

This method is suitable for measurements and comparison of the effectiveness of dentine wounds sealing materials.

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