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Erschienen in: Lasers in Medical Science 6/2020

01.08.2020 | Brief Report

Light emitting diode (LED) based fluorescence microscopy for tuberculosis detection: a review

verfasst von: Anushka Ojha, Soumyabrata Banik, Sindhoora Kaniyala Melanthota, Nirmal Mazumder

Erschienen in: Lasers in Medical Science | Ausgabe 6/2020

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Abstract

Since time immemorial, tuberculosis (TB) has intimidated the human race owing to its severity. Its socio-economic burden has led to it being a major cause of concern. It is one of the world’s major causes of death from a single agent. Since most of the middle- and low-income countries are burdened with TB, sputum smear examination using conventional light microscopy is often the only resort for diagnosing TB. However, fluorescence microscopy is used as standard in most high-income countries, owing to its increased sensitivity. Light-emitting diodes (LEDs), being inexpensive, are increasingly gaining popularity as an alternative light source for fluorescence microscopy. It has been found to be highly efficient and has a lot of advantages over the conventional Ziehl-Neelsen-based bright field microscopy. In this review, we discuss about the usefulness of LED-based fluorescence microscopy in diagnosing TB and how it is superior to the other sources of light used.
Literatur
1.
Zurück zum Zitat Pai M, Behr MA, Dowdy DW, Dheda K, Divangahi M, Boehme CC, Ginsberg A, Swaminathan S, Spigelman M, Getahun H, Menzies D, Raviglione M (2016) Tuberculosis. Nat Rev Dis Primers 2:16076 Pai M, Behr MA, Dowdy DW, Dheda K, Divangahi M, Boehme CC, Ginsberg A, Swaminathan S, Spigelman M, Getahun H, Menzies D, Raviglione M (2016) Tuberculosis. Nat Rev Dis Primers 2:16076
2.
Zurück zum Zitat Glickman MS, Jacobs WR (2001) Microbial pathogenesis of Mycobacterium tuberculosis: dawn of a discipline. Cell 104(4):477–485PubMedCrossRef Glickman MS, Jacobs WR (2001) Microbial pathogenesis of Mycobacterium tuberculosis: dawn of a discipline. Cell 104(4):477–485PubMedCrossRef
5.
Zurück zum Zitat Singhal R, Myneedu VP (2015) Microscopy as a diagnostic tool in pulmonary tuberculosis. Int J Mycobacteriol 4(1):1–6PubMedCrossRef Singhal R, Myneedu VP (2015) Microscopy as a diagnostic tool in pulmonary tuberculosis. Int J Mycobacteriol 4(1):1–6PubMedCrossRef
6.
Zurück zum Zitat Marais BJ, Brittle W, Painczyk K, Hesseling AC, Beyers N, Wasserman E, Soolingen DV, Warren RM (2008) Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum. Clin Infect Dis 47(2):203–207PubMedCrossRef Marais BJ, Brittle W, Painczyk K, Hesseling AC, Beyers N, Wasserman E, Soolingen DV, Warren RM (2008) Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum. Clin Infect Dis 47(2):203–207PubMedCrossRef
7.
Zurück zum Zitat Bhalla M, Sidiq Z, Sharma PP, Singhal R, Myneedu VP, Sarin R (2013) Performance of light-emitting diode fluorescence microscope for diagnosis of tuberculosis. Int J Mycobacteriol 2(3):174–178PubMedCrossRef Bhalla M, Sidiq Z, Sharma PP, Singhal R, Myneedu VP, Sarin R (2013) Performance of light-emitting diode fluorescence microscope for diagnosis of tuberculosis. Int J Mycobacteriol 2(3):174–178PubMedCrossRef
8.
Zurück zum Zitat Khatun Z, Kamal M, Roy CK, Sultana T, Rahman MQ, Azad MBAS, Ahmed ANN (2011) Usefulness of light emitting diode (LED) fluorescent microscopy as a tool for rapid and effective method for the diagnosis of pulmonary tuberculosis. Bangladesh Med Res Counc Bull 37(1):7–10PubMedCrossRef Khatun Z, Kamal M, Roy CK, Sultana T, Rahman MQ, Azad MBAS, Ahmed ANN (2011) Usefulness of light emitting diode (LED) fluorescent microscopy as a tool for rapid and effective method for the diagnosis of pulmonary tuberculosis. Bangladesh Med Res Counc Bull 37(1):7–10PubMedCrossRef
9.
Zurück zum Zitat Khatun Z, Hossain M, Roy C, Sultana T, Rahman MQ, Azad MS, Ahmed ANN (2012) Light emitting diode (LED) fluorescent microscopy: a milestone in the detection of Paucibacillary mycobacterium in case of pulmonary tuberculosis. Bangladesh Med J 40(1):22–26CrossRef Khatun Z, Hossain M, Roy C, Sultana T, Rahman MQ, Azad MS, Ahmed ANN (2012) Light emitting diode (LED) fluorescent microscopy: a milestone in the detection of Paucibacillary mycobacterium in case of pulmonary tuberculosis. Bangladesh Med J 40(1):22–26CrossRef
10.
Zurück zum Zitat World Health Organization (WHO). Global tuberculosis report 2018. Accessed on 10 July 2018 World Health Organization (WHO). Global tuberculosis report 2018. Accessed on 10 July 2018
11.
Zurück zum Zitat Steingart KR, Henry M, Ng V, Hopewell PC, Ramsay A, Cunningham J, Urbanczik R, Perkins M, Aziz MA, Pai M (2006) Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. Lancet Infect Dis 6(9):570–581PubMedCrossRef Steingart KR, Henry M, Ng V, Hopewell PC, Ramsay A, Cunningham J, Urbanczik R, Perkins M, Aziz MA, Pai M (2006) Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. Lancet Infect Dis 6(9):570–581PubMedCrossRef
13.
Zurück zum Zitat Coronel JE, Del Carpio CC, Dianderas EJ, Florentini EA, Kemper GL, Sheen P, Zimic MJ (2019) Evaluation of microbiological variants of sputum processing and concentration of mycobacteria to optimize the microscopic and imaging diagnosis of tuberculosis. International journal of mycobacteriology 8(1):75–82PubMedCrossRef Coronel JE, Del Carpio CC, Dianderas EJ, Florentini EA, Kemper GL, Sheen P, Zimic MJ (2019) Evaluation of microbiological variants of sputum processing and concentration of mycobacteria to optimize the microscopic and imaging diagnosis of tuberculosis. International journal of mycobacteriology 8(1):75–82PubMedCrossRef
14.
Zurück zum Zitat Van Hung N, Sy DN, Anthony RM, Cobelens FG, Van Soolingen D (2007) Fluorescence microscopy for tuberculosis diagnosis. Lancet Infect Dis 7(4):238–239PubMedCrossRef Van Hung N, Sy DN, Anthony RM, Cobelens FG, Van Soolingen D (2007) Fluorescence microscopy for tuberculosis diagnosis. Lancet Infect Dis 7(4):238–239PubMedCrossRef
15.
Zurück zum Zitat Zheludev N (2007) The life and times of the LED—a 100-year history. Nat Photonics 1(4):189–192CrossRef Zheludev N (2007) The life and times of the LED—a 100-year history. Nat Photonics 1(4):189–192CrossRef
18.
Zurück zum Zitat Han K, Yoon S, Chung WJ (2015) CdS and CdSe quantum dot-embedded silicate glasses for LED Color converter. Int J Appl Glas Sci 6(2):103–108 Han K, Yoon S, Chung WJ (2015) CdS and CdSe quantum dot-embedded silicate glasses for LED Color converter. Int J Appl Glas Sci 6(2):103–108
21.
Zurück zum Zitat Krames MR, Shchekin OB, Mueller-Mach R, Mueller GO, Zhou L, Harbers G, Craford MG (2007) Status and future of high-power light-emitting diodes for solid-state lighting. J Disp Techn 3(2):160–175 Krames MR, Shchekin OB, Mueller-Mach R, Mueller GO, Zhou L, Harbers G, Craford MG (2007) Status and future of high-power light-emitting diodes for solid-state lighting. J Disp Techn 3(2):160–175
25.
Zurück zum Zitat Kamtekar KT, Monkman AP, Bryce MR (2010) Recent advances in white organic light-emitting materials and devices (WOLEDs). Adv Mater 22(5):572–582 Kamtekar KT, Monkman AP, Bryce MR (2010) Recent advances in white organic light-emitting materials and devices (WOLEDs). Adv Mater 22(5):572–582
27.
Zurück zum Zitat Robertson JB, Zhang Y, Johnson CH (2009) Light-emitting diode flashlights as effective and inexpensive light sources for fluorescence microscopy. J Microsc 236(1):1–4 Robertson JB, Zhang Y, Johnson CH (2009) Light-emitting diode flashlights as effective and inexpensive light sources for fluorescence microscopy. J Microsc 236(1):1–4
28.
Zurück zum Zitat Beacher J (2008) LEDs for fluorescence microscopy-LEDs offer reliability and repeatability for fluorescence applications. Photonics Spectra 19:13 Beacher J (2008) LEDs for fluorescence microscopy-LEDs offer reliability and repeatability for fluorescence applications. Photonics Spectra 19:13
29.
Zurück zum Zitat Aswani KK (2016) Emerging LED technologies for fluorescence microscopy. Microsc Today 24(4):22–27CrossRef Aswani KK (2016) Emerging LED technologies for fluorescence microscopy. Microsc Today 24(4):22–27CrossRef
30.
Zurück zum Zitat Nakamura S (2015) Nobel lecture: background story of the invention of efficient blue InGaN light emitting diodes. Rev Mod Phys 87(4):1139 Nakamura S (2015) Nobel lecture: background story of the invention of efficient blue InGaN light emitting diodes. Rev Mod Phys 87(4):1139
31.
Zurück zum Zitat Svelto O (2010) Principles of lasers, 5th edn. Springer, New York Svelto O (2010) Principles of lasers, 5th edn. Springer, New York
34.
Zurück zum Zitat Miller AR, Davis GL, Oden ZM, Razavi MR, Fateh A, Ghazanfari M, Abdolrahimi F, Poorazar S, Sakhaie F, Olsen RJ, & Bahrmand AR. Portable, battery-operated, low-cost, bright field and fluorescence microscope. PloS one 5(8):e11890 Miller AR, Davis GL, Oden ZM, Razavi MR, Fateh A, Ghazanfari M, Abdolrahimi F, Poorazar S, Sakhaie F, Olsen RJ, & Bahrmand AR. Portable, battery-operated, low-cost, bright field and fluorescence microscope. PloS one 5(8):e11890
35.
Zurück zum Zitat Reza LW, Satyanarayna S, Enarson DA, Kumar AM, Sagili K, Kumar S, Prabhakar LA, Devendrappa NM, Pandey A, Wilson N, Chadha S (2013) LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India. PLoS One 8(10):e75566PubMedPubMedCentralCrossRef Reza LW, Satyanarayna S, Enarson DA, Kumar AM, Sagili K, Kumar S, Prabhakar LA, Devendrappa NM, Pandey A, Wilson N, Chadha S (2013) LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India. PLoS One 8(10):e75566PubMedPubMedCentralCrossRef
37.
Zurück zum Zitat Hänscheid T (2008) The future looks bright: low-cost fluorescent microscopes for detection of Mycobacterium tuberculosis and Coccidiae. Trans R Soc Trop Med Hyg 102(6):520–521PubMedCrossRef Hänscheid T (2008) The future looks bright: low-cost fluorescent microscopes for detection of Mycobacterium tuberculosis and Coccidiae. Trans R Soc Trop Med Hyg 102(6):520–521PubMedCrossRef
38.
Zurück zum Zitat Minion J, Pai M, Ramsay A, Menzies D, Greenaway C (2011) Comparison of LED and conventional fluorescence microscopy for detection of acid fast bacilli in a low-incidence setting. PLoS One 6(7):e22495PubMedPubMedCentralCrossRef Minion J, Pai M, Ramsay A, Menzies D, Greenaway C (2011) Comparison of LED and conventional fluorescence microscopy for detection of acid fast bacilli in a low-incidence setting. PLoS One 6(7):e22495PubMedPubMedCentralCrossRef
39.
40.
Zurück zum Zitat Shah MI, Mishra S, Sarkar M, Sudarshan SK (2016) Automatic detection and classification of tuberculosis bacilli from camera-enabled smartphone microscopic images. In parallel, distributed and grid computing (PDGC), 2016 fourth international conference on (pp. 287-290). IEEE Shah MI, Mishra S, Sarkar M, Sudarshan SK (2016) Automatic detection and classification of tuberculosis bacilli from camera-enabled smartphone microscopic images. In parallel, distributed and grid computing (PDGC), 2016 fourth international conference on (pp. 287-290). IEEE
Metadaten
Titel
Light emitting diode (LED) based fluorescence microscopy for tuberculosis detection: a review
verfasst von
Anushka Ojha
Soumyabrata Banik
Sindhoora Kaniyala Melanthota
Nirmal Mazumder
Publikationsdatum
01.08.2020
Verlag
Springer London
Erschienen in
Lasers in Medical Science / Ausgabe 6/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-019-02947-6

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