Background
Methods
Generation and breeding of Cntn4 mice
Age | Task | Batch | Subjects (n) |
---|---|---|---|
3 weeks | Juvenile social interaction | Batch 1 + 2 | 6–7 genotype-matched pairs per genotype |
4 weeks | Extended SHIRPA screen | Batch 1 + 2 | 19–26 per genotype |
6 weeks | Extended SHIRPA screen | Batch 1 + 2 | 19–26 per genotype |
8 weeks | Extended SHIRPA screen | Batch 1 + 2 | 19–26 per genotype |
Adult | Extended SHIRPA screen | Batch 1 | 10–15 per genotype |
Open field | Batch 1 | 10–15 per genotype | |
Elevated plus maze | Batch 1 | 10–15 per genotype | |
Social discrimination | Batch 1 | 10–15 per genotype | |
Buried food test | Batch 2 | 9–11 per genotype | |
Set shifting-reversal task | Batch 2 | 9–11 per genotype | |
Social approach in 3-chamber | Batch 3 | 12 per genotype | |
Novel object exploration task | Batch 3 | 12 per genotype | |
Barnes maze-reversal task | Batch 4 | 16 per genotype | |
Pre-pulse inhibition | Batch 4 | 16 per genotype |
Developmental neurological and behavioral screening
Screening of social behaviors and restricted repetitive behaviors
Results
eSHIRPA assays
Age (weeks) | 4 | 6 | 8 | Adult | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Test | WT | +/− | −/− | Sig. | WT | +/− | −/− | Sig. | WT | +/− | −/− | Sig. | WT | +/− | −/− | Sig. |
Subjects (n) | 19 | 26 | 22 | 19 | 26 | 22 | 19 | 26 | 22 | 13 | 15 | 10 | ||||
Body position (active) | 95 | 96 | 100 | 0.22 | 100 | 100 | 100 | 1.00 | 100 | 96 | 100 | 0.46 | 100 | 100 | 100 | 1.00 |
Body position (inactive) | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | ||||
Body position (excessive activity) | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||
Tremor (absent) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Palpebral closure (eyes open) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Coat appearance tidy and groomed | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Whiskers (present) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Lacrimation (absent) | 100 | 96 | 100 | 0.45 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Defecation (quantity) | 1.9 | 1.6 | 2.0 | 0.47 | 3.1 | 2.5 | 1.9 | 0.06 | 2.8 | 2.3 | 2.4 | 0.61 | 3.8 | 3.7 | 3.2 | 0.70 |
± SEM | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.4 | 0.3 | 0.4 | 0.7 | 0.7 | 0.9 | ||||
Transfer arousal (brief freeze) | 11 | 15 | 14 | 0.70 | 11 | 19 | 9 | 0.71 | 11 | 8 | 0 | 0.16 | 8 | 0 | 10 | 0.50 |
Transfer arousal (immediate movement) | 89 | 85 | 86 | 89 | 81 | 91 | 89 | 92 | 100 | 92 | 100 | 90 | ||||
Gait (fluid) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Tail elevation (horizontal extension) | 100 | 100 | 100 | 1.00 | 95 | 100 | 100 | 0.28 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Tail elevation (straub tail) | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||
Startle response (preyer reflex) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Touch escape (flight prior to touch) | 79 | 73 | 77 | 0.88 | 89 | 85 | 73 | 0.35 | 63 | 88 | 68 | 0.05 | 85 | 87 | 100 | 0.45 |
Touch escape (response to touch) | 21 | 27 | 23 | 11 | 15 | 27 | 37 | 12 | 32 | 15 | 13 | 0 | ||||
Positional passivity (struggles) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Skin color (pink) | 95 | 100 | 100 | 0.28 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Skin color (blanched) | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||
Trunk curl (absent) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Limb grasping (absent) | 100 | 92 | 100 | 0.20 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Pinna reflex (present) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Corneal reflex (present) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Contact righting reflex (present) | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Evidence of biting (none) | 79 | 77 | 77 | 0.99 | 84 | 88 | 100 | 0.18 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |
Grip (OK) | 100 | 96 | 100 | 0.36 | 95 | 96 | 100 | 0.59 | 100 | 100 | 100 | 1.00 | 100 | 100 | 90 | 0.24 |
Full puberty | 89 | 81 | 100 | 0.30 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 | 100 | 100 | 100 | 1.00 |