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Strahlentherapie und Onkologie

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04.08.2022 | Original Article

Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes

verfasst von: Ravindra M Samarth, Puneet Gandhi, Nabo Kumar Chaudhury

Erschienen in: Strahlentherapie und Onkologie | Ausgabe 2/2023

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Abstract

Purpose

The frequency of acrocentric chromosome associations (ACA) was studied to determine the possible dose–response relationship of gamma irradiation in human lymphocytes.

Methods

Peripheral blood collected from three healthy donors was irradiated with 0, 0.1, 0.25, 0.5, 0.75, 1, 2, 3, 4, and 5 Gy of gamma radiation. Chromosomal preparations were made after 48 h of culture as per standard guidelines. The experiment was repeated three times, with a different donor each time.

Results

The ACA frequency in irradiated lymphocytes increased with radiation dose. The D–G type of association was most prominent and showed a significant dose-dependent increase in frequency. The dose response of ACA frequency to radiation was found to be linear: ACA frequency = 0.2923 (±0.0276) + 0.1846 (±0.0307) × D (correlation coefficient r = 0.9442). As expected, dicentric chromosome (DC) frequencies followed the linear quadratic fit model, with DC frequency = 0.0015 (±0.0013) + 0.0220 (±0.0059) × D + 0.0215 (±0.0018) × D^2 (correlation coefficient r = 0.9982). A correlation curve was prepared for ACA frequency versus DC frequency, resulting in the regression equation y = 1.130x + 0.4051 (R² = 0.7408; p = 0.0014).

Conclusion

Our results showed an increase in ACA frequency in irradiated lymphocytes with an increase in radiation dose; thus, ACA may serve as a candidate cytogenetic biomarker for radiation biodosimetry.

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Titel: Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes
verfasst von: Ravindra M Samarth
Puneet Gandhi
Nabo Kumar Chaudhury
Publikationsdatum: 04.08.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: Strahlentherapie und Onkologie / Ausgabe 2/2023
Print ISSN: 0179-7158
Elektronische ISSN: 1439-099X
DOI: https://doi.org/10.1007/s00066-022-01978-3

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Linear dose response of acrocentric chromosome associations to gamma irradiation in human lymphocytes

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Purpose

Methods

Results

Conclusion

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