Notes & TipsDetermination of Heme Oxygenase Activity in Murine Macrophages for Studying Oxidative Stress Inhibitors☆
References (12)
- et al.
Methods Enzymol.
(1994) - et al.
J. Biol. Chem.
(1978) - et al.
Anal. Biochem.
(1988) - et al.
Anal. Biochem.
(1992) - et al.
FEBS Lett.
(1971)
Cited by (31)
Cucurbitacins attenuate microglial activation and protect from neuroinflammatory injury through Nrf2/ARE activation and STAT/NF-κB inhibition
2015, Neuroscience LettersCitation Excerpt :TNF-α, IL-1β, and IL-6 levels in the culture media were determined using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN), according to the manufacturer's instructions. HO-1 activity was determined by measuring the amount of bilirubin, as previously described [21]. Briefly, 500 μL of each cell culture supernatant was collected and mixed with 250 mg barium chloride dihydrate (BaCl2·2H2O) by vortexing (10–15 s).
Ethyl linoleate from garlic attenuates lipopolysaccharide-induced pro-inflammatory cytokine production by inducing heme oxygenase-1 in RAW264.7 cells
2014, International ImmunopharmacologyCitation Excerpt :Images were acquired using an ImageQuant 350 analyzer (Amersham Biosciences) and densitometry was performed using the ImageQuant TL software. HO-1 activity was determined by measuring the amount of bilirubin as previously described [21]. Briefly, 500 μL of each culture supernatant was collected and mixed with 250 mg barium chloride dihydrate (BaCl2·2H2O) by vortexing (10–15 s).
Involvement of PKA and HO-1 signaling in anti-inflammatory effects of surfactin in BV-2 microglial cells
2013, Toxicology and Applied PharmacologyCitation Excerpt :Nuclei were stained with 1 μg/ml of 4′-6-diamidino-2-phenylindole (DAPI), and then analyzed by confocal microscopy using a Zeiss LSM 510 Meta microscope (Zeiss, Jena, Germany). The HO-1 activity was determined by measuring the amount of bilirubin as described previously (Turcanu et al., 1998). To determine the bilirubin levels, 500 μl of each culture supernatant was collected and mixed with 250 mg barium chloride dihydrate (BaCl2·2H2O) by vortexing (10–15 s).
Induction of heme oxygenase-1 expression by cilostazol contributes to its anti-inflammatory effects in J774 murine macrophages
2011, Immunology LettersCitation Excerpt :The results are expressed as a relative density. Bilirubin was determined as described by Turcanu et al. [19]. J774A.1 macrophages were preincubated with hemin (50 μM) for 2 h and thereafter treated with cilostazol (10 μM) or CoPP (1 μM) for 24 h, and then bilirubin was determined in the culture medium.
Heme oxygenase expression and activity in immortalized hypothalamic neurons GT1-7
2008, Neuroscience Letters
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De Matteis, F.Norman Aldridge, W.
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