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Bacterial Proteases and Adherence ofPseudomonas aeruginosato Mouse Cornea

https://doi.org/10.1006/exer.1996.0075Get rights and content

Abstract

The goal of this study was to test whether bacterial exoproducts, such as elastase or alkaline protease contribute to the initial binding ofPseudomonas aeruginosato mouse corneal epithelium. Each protease, purified fromP. aeruginosa,when applied exogenously at concentrations of either 25 or 50ngml−1, elevated binding ofPseudomonasto mouse cornea in organ culture. Polyclonal antibodies against bacterial alkaline protease, but not elastase, interfered with bacterial binding and reduced significantly the number of organisms bound to cornea in an organ culture binding inhibition assay. Zymographic analysis of conditioned media from additional organ culture experiments showed that theP. aeruginosastrain used, which is highly virulent in cornea in vivo, secretes detectable levels of alkaline protease, but not elastase in vitro and that secretion was enhanced if the corneal epithelium was wounded. Lastly, how alkaline protease enhanced bacterial binding to the corneal epithelium of the organ cultured eye was examined. Data from this study suggest that exposure of lipase-sensitive epithelial receptors represents at least one mechanism.

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For correspondence at: Wayne State University, Department of Anatomy/Cell Biology, 540 East Canfield Avenue, Detroit, MI 48201, U.S.A.

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