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Int J Angiol 1995; 4(1): 25-30
DOI: 10.1007/BF02043502
Original Articles

© Georg Thieme Verlag KG Stuttgart · New York

Near infrared spectroscopy: Blood and tissue oxygenation in renal ischemia-reperfusion injury in rats

Debra L. Vaughan1 , Yapa A. B. D. Wickramasinghe1 , Gavin I. Russell1 , Maureen S. Thorniley2 , Ralph F. Houston2 , Ernie Ruban3 , Peter Rolfe2
  • 1Renal Research, Stoke-on-Trent, United Kingdom
  • 2Department of Bioengineering and Medical Physics, School of Postgraduate Medicine, Keele University, Hartshill, Stoke-on-Trent, United Kingdom
  • 3Department of Histology, North Staffordshire Pathology Services, North Staffordshire Hospital, Hartshill, Stoke-on-Trent, United Kingdom
Presented at The 35th World Congress, International College of Angiology, Copenhagen, Denmark, July 1993
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Publication Date:
22 April 2011 (online)

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Abstract

Near infrared spectroscopy was used to monitor blood (HbO2, Hb, and Hbtot) and tissue oxygenation (oxidized cyt aa3) of both left and right kidneys of rats in vivo simultaneously, during either 45 or 80 minutes of left renal ischemia followed by 4.5 hours of reperfusion. Ischemia significantly reduced HbO2 (p< 0.0001) and increased Hb (p<0.05) concentration change in the left kidney compared with the right kidney (control). Reperfusion with oxygenated blood reached control levels after 1 hour. The rate of change in HbO2 (p<0.05) and Hbtot (p<0.05) concentration during reperfusion was dependent upon the duration of ischemia, being slower after the longer ischemic period of 80 minutes. The concentration change of oxidized cyt aa3 of the 80-minute ischemic group slowly increased compared with the stable redox state of the 45-minute ischemic group, during the latter stages of reperfusion (p<0.05). Near infrared spectroscopy is a promising new development that will enable the effects of interventional treatment upon ischemia and reperfusion injury to be studied.

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