Abstract
Historically, brown adipose tissue has been elusive and not easy to detect, hence its relative obscurity in human physiology until its rediscovery in 2009. At that point, it was proven that the symmetrical artefacts frequently detected on positron emission tomography-computed tomography (PET-CT), which resolved if the environment was kept warm, were brown adipose tissue deposits. PET-CT has remained the stalwart of human brown adipose tissue research and is still considered the gold standard. However, PET-CT exposes the participant to ionising radiation, limiting studies to large, but retrospective, review of clinical imaging or a small-scale, but prospective, design. Within this context, alternative imaging modalities have been sought. Due to the heat-generating properties of brown adipose tissue, infrared thermography is a natural candidate for measuring its activity and the supraclavicular depot is relatively superficial, allowing detection of the heat signature. Infrared thermography is a non-invasive, non-contact technique for measuring temperature remotely. Recent developments in image analysis techniques have facilitated the use of infrared thermography to study brown adipose tissue activation in populations, and in ways, not previously feasible.
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References
Alexander G, Stevens D (1980) Sympathetic innervation and the development of structure and function of brown adipose tissue: studies on lambs chemically sympathectomized in utero with 6-hydroxydopamine. J Dev Physiol 2:119–137
Ang QY, Goh HJ, Cao Y, Li Y, Chan SP, Swain JL, Henry CJ, Leow MK (2017) A new method of infrared thermography for quantification of brown adipose tissue activation in healthy adults (TACTICAL): a randomized trial. J Physiol Sci 67:395–406. https://doi.org/10.1007/s12576-016-0472-1
Arora N, Martins D, Ruggerio D, Tousimis E, Swistel AJ, Osborne MP, Simmons RM (2008) Effectiveness of a noninvasive digital infrared thermal imaging system in the detection of breast cancer. Am J Surg 196:523–526. https://doi.org/10.1016/j.amjsurg.2008.06.015
Baldwin JE, Harris CS, Ryle M (1973) 5 GHz observations of the infrared star MWC 349, and the H II condensation W3(OH). Nature 241:38–39
Braverman IM (2000) The cutaneous microcirculation. J Investig Dermatol Symp Proc 5:3–9. https://doi.org/10.1046/j.1087-0024.2000.00010.x
Candoré JC, Bodnar JL, Detalle V, Grossel P (2012) Non-destructive testing of works of art by stimulated infrared thermography. Eur Phys J Appl Phys 57:21002. https://doi.org/10.1051/epjap/2011110266
Cannon B, Nedergaard J (2004) Brown adipose tissue: function and physiological significance. Physiol Rev 84:277–359. https://doi.org/10.1152/physrev.00015.2003
Celi FS (2009) Brown adipose tissue--when it pays to be inefficient. N Engl J Med 360:1553–1556. https://doi.org/10.1056/NEJMe0900466
Clark MR, McCann DM, Forde MC (2003) Application of infrared thermography to the non-destructive testing of concrete and masonry bridges. NDT E Int 36:265–275. https://doi.org/10.1016/S0963-8695(02)00060-9
Collins AJ, Cosh JA (1970) Temperature and biochemical studies of joint inflammation. A preliminary investigation. Ann Rheum Dis 29:386
Cypess AM, Lehman S, Williams G, Tal I, Rodman D, Goldfine AB, Kuo FC, Palmer EL, Tseng YH, Doria A, Kolodny GM, Kahn CR (2009) Identification and importance of brown adipose tissue in adult humans. N Engl J Med 360:1509–1517. https://doi.org/10.1056/NEJMoa0810780
Davis TR (1961) Chamber cold acclimatization in man. J Appl Physiol 16:1011–1015
El Hadi H, Frascati A, Granzotto M, Silvestrin V, Ferlini E, Vettor R, Rossato M (2016) Infrared thermography for indirect assessment of activation of brown adipose tissue in lean and obese male subjects. Physiol Meas 37:N118–N128. https://doi.org/10.1088/0967-3334/37/12/n118
Engel JM (1984) Physical and physiological influence of medical ointments on infrared thermography. In: Ring EFJ, Phillips B (eds) Recent advances in medical thermology. Springer, New York, pp 177–183
Fernández-Cuevas I, Bouzas Marins JC, Arnáiz Lastras J, Gómez Carmona PM, Piñonosa Cano S, García-Concepción MÁ, Sillero-Quintana M (2015) Classification of factors influencing the use of infrared thermography in humans: a review. Infrared Phys Technol 71:28–55. https://doi.org/10.1016/j.infrared.2015.02.007
Gatidis S, Schmidt H, Pfannenberg CA, Nikolaou K, Schick F, Schwenzer NF (2016) Is it possible to detect activated brown adipose tissue in humans using single-time-point infrared thermography under thermoneutral conditions? Impact of BMI and subcutaneous adipose tissue thickness. PLoS One 11:e0151152. https://doi.org/10.1371/journal.pone.0151152
Grassi G, Seravalle G, Calhoun DA, Bolla G, Mancia G (1992) Cigarette smoking and the adrenergic nervous system. Clin Exp Hypertens A Theory Pract 14:251–260. https://doi.org/10.3109/10641969209036186
Haq T, Crane JD, Kanji S, Gunn E, Tarnopolsky MA, Gerstein HC, Steinberg GR, Morrison KM (2017) Optimizing the methodology for measuring supraclavicular skin temperature using infrared thermography; implications for measuring brown adipose tissue activity in humans. Sci Rep 7:11934. https://doi.org/10.1038/s41598-017-11537-x
Heaton JM (1972) The distribution of brown adipose tissue in the human. J Anat 112:35–39
Herschel W (1800) Experiments on the refrangibility of the invisible rays of the Sun. By William Herschel, LL. D. F. R. S. Philos Trans R Soc Lond 90:284–292. https://doi.org/10.1098/rstl.1800.0015
Hildebrandt C, Zeilberger K, Ring EFJ, Raschner C (2012) The application of medical infrared thermography in sports medicine. In: Zaslav KR (ed) An international perspective on topics in sports medicine and sports injury. InTech, Rijeka
Houdas Y, Ring EFJ (1982) Human body temperature: its measurement and regulation. Springer, New York
Jang C, Jalapu S, Thuzar M, Law PW, Jeavons S, Barclay JL, Ho KKY (2014) Infrared thermography in the detection of brown adipose tissue in humans. Physiol Rep 2:e12167. https://doi.org/10.14814/phy2.12167
Kim MS, Hu HH, Aggabao PC, Geffner ME, Gilsanz V (2014) Presence of brown adipose tissue in an adolescent with severe primary hypothyroidism. J Clin Endocrinol Metab 99:E1686–E1690. https://doi.org/10.1210/jc.2014-1343
Kim M, Goto T, Yu R, Uchida K, Tominaga M, Kano Y, Takahashi N, Kawada T (2015) Fish oil intake induces UCP1 upregulation in brown and white adipose tissue via the sympathetic nervous system. Sci Rep 5:18013. https://doi.org/10.1038/srep18013
King DL, Kratochvil JA, Quintana MA, McMahon TJ (2000) Applications for infrared imaging equipment in photovoltaic cell, module, and system testing. In: Conference record of the twenty-eighth IEEE photovoltaic specialists conference – 2000 (Cat. No.00CH37036), IEEE, Anchorage, pp 1487–1490
Kontos M, Wilson R, Fentiman I (2011) Digital infrared thermal imaging (DITI) of breast lesions: sensitivity and specificity of detection of primary breast cancers. Clin Radiol 66:536–539. https://doi.org/10.1016/j.crad.2011.01.009
Law J, Bloor I, Budge H, Symonds ME (2014) The influence of sex steroids on adipose tissue growth and function. Horm Mol Biol Clin Invest 19:13–24. https://doi.org/10.1515/hmbci-2014-0015
Law J, Chalmers J, Morris DE, Robinson L, Budge H, Symonds ME (2017a) The use of infrared thermography in the measurement and characterization of brown adipose tissue activation. Temperature:1–15. https://doi.org/10.1080/23328940.2017.1397085
Law JM, Morris DE, Izzi-Engbeaya C, Salem V, Coello C, Robinson L, Jayasinghe M, Scott R, Gunn R, Rabiner E, Tan T, Dhillo W, Bloom S, Budge H, Symonds M (2017b) Thermal imaging is a non-invasive alternative to PET-CT for measurement of brown adipose tissue activity in humans. J Nucl Med. https://doi.org/10.2967/jnumed.117.190546
Lee P, Ho KK, Lee P, Greenfield JR, Ho KK, Greenfield JR (2011) Hot fat in a cool man: infrared thermography and brown adipose tissue. Diabetes Obes Metab 13:92–93. https://doi.org/10.1111/j.1463-1326.2010.01318.x
Leitner BP, Huang S, Brychta RJ, Duckworth CJ, Baskin AS, McGehee S, Tal I, Dieckmann W, Gupta G, Kolodny GM, Pacak K, Herscovitch P, Cypess AM, Chen KY (2017) Mapping of human brown adipose tissue in lean and obese young men. Proc Natl Acad Sci U S A 114:8649–8654. https://doi.org/10.1073/pnas.1705287114
Moreira DG, Costello JT, Brito CJ, Adamczyk JG, Ammer K, Bach AJE, Costa CMA, Eglin C, Fernandes AA, Fernández-Cuevas I, Ferreira JJA, Formenti D, Fournet D, Havenith G, Howell K, Jung A, Kenny GP, Kolosovas-Machuca ES, Maley MJ, Merla A, Pascoe DD, Priego Quesada JI, Schwartz RG, Seixas ARD, Selfe J, Vainer BG, Sillero-Quintana M (2017) Thermographic imaging in sports and exercise medicine: a Delphi study and consensus statement on the measurement of human skin temperature. J Therm Biol 69:155–162. https://doi.org/10.1016/j.jtherbio.2017.07.006
Ootsuka Y, Tanaka M (2015) Control of cutaneous blood flow by central nervous system. Temperature 2:392–405. https://doi.org/10.1080/23328940.2015.1069437
Peterson CM, Orooji M, Johnson DN, Naraghi-Pour M, Ravussin E (2017) Brown adipose tissue does not seem to mediate metabolic adaptation to overfeeding in men. Obesity 25:502–505. https://doi.org/10.1002/oby.21721
Planck M (1914) The theory of heat radiation. P. Blakiston’s Son & Co., Philadelphia
Ramage LE, Akyol M, Fletcher Alison M, Forsythe J, Nixon M, Carter Roderick N, van Beek Edwin JR, Morton Nicholas M, Walker Brian R, Stimson Roland H (2016) Glucocorticoids acutely increase brown adipose tissue activity in humans, revealing species-specific differences in UCP-1 regulation. Cell Metab 24:130–141. https://doi.org/10.1016/j.cmet.2016.06.011
Redlin U, Nuesslein B, Schmidt I (1992) Circadian changes of brown adipose tissue thermogenesis in juvenile rats. Am J Phys Regul Integr Comp Phys 262:R504–R508
Ring EFJ, Ammer K (2012) Infrared thermal imaging in medicine. Physiol Meas 33:R33. https://doi.org/10.1088/0967-3334/33/3/R33
Ring EFJ, Collins AJ (1970) Quantitative thermography. Rheumatology 10:337–341. https://doi.org/10.1093/rheumatology/10.7.337
Roberts RE, Selby JEA, Biberman LM (1976) Infrared continuum absorption by atmospheric water vapor in the 8–12-μm window. Appl Opt 15:2085–2090. https://doi.org/10.1364/AO.15.002085
Robinson L, Ojha S, Symonds ME, Budge H (2014) Body mass index as a determinant of brown adipose tissue function in healthy children. J Pediatr 164:318–322. https://doi.org/10.1016/j.jpeds.2013.10.005
Robinson LJ, Law JM, Symonds ME, Budge H (2016) Brown adipose tissue activation as measured by infrared thermography by mild anticipatory psychological stress in lean healthy females. Exp Physiol 101:549–557. https://doi.org/10.1113/ep085642
Rothwell NJ, Stock MJ (1979) A role for brown adipose tissue in diet-induced thermogenesis. Nature 281:31–35. https://doi.org/10.1038/281031a0
Salem V, Izzi-Engbeaya C, Coello C, Thomas DB, Chambers ES, Comninos AN, Buckley A, Win Z, Al-Nahhas A, Rabiner EA, Gunn RN, Budge H, Symonds ME, Bloom SR, Tan TM, Dhillo WS (2016) Glucagon increases energy expenditure independently of brown adipose tissue activation in humans. Diabetes Obes Metab 18:72–81. https://doi.org/10.1111/dom.12585
Scotney H, Symonds ME, Law J, Budge H, Sharkey D, Manolopoulos KN (2017) Glucocorticoids modulate human brown adipose tissue thermogenesis in vivo. Metab Clin Exp 70:125–132. https://doi.org/10.1016/j.metabol.2017.01.024
Smith RE, Horwitz BA (1969) Brown fat and thermogenesis. Physiol Rev 49:330–425
Steketee J (1973) Spectral emissivity of skin and pericardium. Phys Med Biol 18:686
Steketee J (1976) The influence of cosmetics and ointments on the spectral emissivity of skin (skin temperature measurement). Phys Med Biol 21:920
Symonds ME, Henderson K, Elvidge L, Bosman C, Sharkey D, Perkins AC, Budge H (2012) Thermal imaging to assess age-related changes of skin temperature within the supraclavicular region co-locating with brown adipose tissue in healthy children. J Pediatr 161:892–898. https://doi.org/10.1016/j.jpeds.2012.04.056
Tattersall GJ (2016) Infrared thermography: a non-invasive window into thermal physiology. Comp Biochem Physiol A Mol Integr Physiol 202:78–98. https://doi.org/10.1016/j.cbpa.2016.02.022
Thureson-Klein Å, Lagercrantz H, Barnard T (1976) Chemical sympathectomy of interscapular brown adipose tissue. Acta Physiol Scand 98:8–18. https://doi.org/10.1111/j.1748-1716.1976.tb10296.x
van der Lans AAJJ, Hoeks J, Brans B, Vijgen GHEJ, Visser MGW, Vosselman MJ, Hansen J, Jörgensen JA, Wu J, Mottaghy FM, Schrauwen P, van Marken Lichtenbelt WD (2013) Cold acclimation recruits human brown fat and increases nonshivering thermogenesis. J Clin Investig 123:3395–3403. https://doi.org/10.1172/JCI68993
van der Lans AAJJ, Wierts R, Vosselman MJ, Schrauwen P, Brans B, van Marken Lichtenbelt WD (2014) Cold-activated brown adipose tissue in human adults: methodological issues. Am J Physiol Regul Integr Comp Physiol 307:R103–R113. https://doi.org/10.1152/ajpregu.00021.2014
van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM, Drossaerts JM, Kemerink GJ, Bouvy ND, Schrauwen P, Teule GJ (2009) Cold-activated brown adipose tissue in healthy men. N Engl J Med 360:1500–1508. https://doi.org/10.1056/NEJMoa0808718
Virtanen KA, Lidell ME, Orava J, Heglind M, Westergren R, Niemi T, Taittonen M, Laine J, Savisto NJ, Enerback S, Nuutila P (2009) Functional brown adipose tissue in healthy adults. N Engl J Med 360:1518–1525. https://doi.org/10.1056/NEJMoa0808949
Vosselman MJ, van der Lans AAJJ, Brans B, Wierts R, van Baak MA, Schrauwen P, van Marken Lichtenbelt WD (2012) Systemic β-adrenergic stimulation of thermogenesis is not accompanied by brown adipose tissue activity in humans. Diabetes 61:3106–3113. https://doi.org/10.2337/db12-0288
Vosselman MJ, Brans B, van der Lans AA, Wierts R, van Baak MA, Mottaghy FM, Schrauwen P, van Marken Lichtenbelt WD (2013) Brown adipose tissue activity after a high-calorie meal in humans. Am J Clin Nutr 98:57–64. https://doi.org/10.3945/ajcn.113.059022
Vrieze A, Schopman JE, Admiraal WM, Soeters MR, Nieuwdorp M, Verberne HJ, Holleman F (2012) Fasting and postprandial activity of brown adipose tissue in healthy men. J Nucl Med 53:1407–1410. https://doi.org/10.2967/jnumed.111.100701
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Law, J., Morris, D.E., Budge, H., Symonds, M.E. (2018). Infrared Thermography. In: Pfeifer, A., Klingenspor, M., Herzig, S. (eds) Brown Adipose Tissue. Handbook of Experimental Pharmacology, vol 251. Springer, Cham. https://doi.org/10.1007/164_2018_137
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